In vitro catabolism of human plasma very low density lipoproteins. Effects of VLDL concentration on the interconversion of high density lipoprotein subfractions

Marja Riitta Taskinen, Moti L. Kashyap, Laxmi S. Srivastava, Muhammad Ashraf, J. David Johnson, Gladys Perisutti, David Brady, Charles J. Glueck, Richard L. Jackson

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The effect of lipolysis of human plasma very low density lipoproteins (VLDL) on the distribution of high density lipoprotein subfractions was studied in an in vitro system consisting of purified bovine milk lipoprotein lipase and albumin. The distribution of lipids and apoproteins (apoC-II and apoC-III) within the lipoprotein fractions corresponding to HDL2 (d = 1.063-1.120 g/ml) and HDL3 (d = 1.120-1.210 g/ml) was dependent upon the concentration of VLDL in the incubation mixture. After lipolysis of an incubation mixture containing VLDL-triglyceride (0.6 mg triglyceride/ml) and HDL3 (0.1 mg protein/ml), most of the lipid and apoproteins were recovered in HDL3. At higher concentrations of VLDL-triglyceride relative to HDL3-protein (1.8 or 2.4 mg of VLDL-triglyceride and 0.1 mg of HDL3-protein) the amount of lipid and apoprotein isolated in the HDL3 density fraction decreased after lipolysis and there was an increase in the amount isolated between d 1.063-1.120 g/ml. These results provide additional evidence for the conversion of HDL3 to HDL2 during lipolysis. Furthermore, they suggest that the relative distribution of plasma HDL2 and HDL3 is related to the rate of catabolism of triglyceride-rich lipoproteins.

Original languageEnglish (US)
Pages (from-to)381-394
Number of pages14
JournalAtherosclerosis
Volume41
Issue number2-3
DOIs
StatePublished - Jan 1 1982
Externally publishedYes

Fingerprint

VLDL Lipoproteins
Lipolysis
HDL Lipoproteins
Apoproteins
Lipids
Lipoproteins
Triglycerides
Apolipoprotein C-III
Apolipoproteins C
Proteins
Lipoprotein Lipase
Albumins
Milk
In Vitro Techniques
very low density lipoprotein triglyceride

Keywords

  • HDL cholesterol
  • Lipolysis
  • Lipoprotein lipase
  • Lipoprotein metabolism

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

In vitro catabolism of human plasma very low density lipoproteins. Effects of VLDL concentration on the interconversion of high density lipoprotein subfractions. / Taskinen, Marja Riitta; Kashyap, Moti L.; Srivastava, Laxmi S.; Ashraf, Muhammad; David Johnson, J.; Perisutti, Gladys; Brady, David; Glueck, Charles J.; Jackson, Richard L.

In: Atherosclerosis, Vol. 41, No. 2-3, 01.01.1982, p. 381-394.

Research output: Contribution to journalArticle

Taskinen, Marja Riitta ; Kashyap, Moti L. ; Srivastava, Laxmi S. ; Ashraf, Muhammad ; David Johnson, J. ; Perisutti, Gladys ; Brady, David ; Glueck, Charles J. ; Jackson, Richard L. / In vitro catabolism of human plasma very low density lipoproteins. Effects of VLDL concentration on the interconversion of high density lipoprotein subfractions. In: Atherosclerosis. 1982 ; Vol. 41, No. 2-3. pp. 381-394.
@article{5580538d0c5a4d9d9aff0dc4c48e27dd,
title = "In vitro catabolism of human plasma very low density lipoproteins. Effects of VLDL concentration on the interconversion of high density lipoprotein subfractions",
abstract = "The effect of lipolysis of human plasma very low density lipoproteins (VLDL) on the distribution of high density lipoprotein subfractions was studied in an in vitro system consisting of purified bovine milk lipoprotein lipase and albumin. The distribution of lipids and apoproteins (apoC-II and apoC-III) within the lipoprotein fractions corresponding to HDL2 (d = 1.063-1.120 g/ml) and HDL3 (d = 1.120-1.210 g/ml) was dependent upon the concentration of VLDL in the incubation mixture. After lipolysis of an incubation mixture containing VLDL-triglyceride (0.6 mg triglyceride/ml) and HDL3 (0.1 mg protein/ml), most of the lipid and apoproteins were recovered in HDL3. At higher concentrations of VLDL-triglyceride relative to HDL3-protein (1.8 or 2.4 mg of VLDL-triglyceride and 0.1 mg of HDL3-protein) the amount of lipid and apoprotein isolated in the HDL3 density fraction decreased after lipolysis and there was an increase in the amount isolated between d 1.063-1.120 g/ml. These results provide additional evidence for the conversion of HDL3 to HDL2 during lipolysis. Furthermore, they suggest that the relative distribution of plasma HDL2 and HDL3 is related to the rate of catabolism of triglyceride-rich lipoproteins.",
keywords = "HDL cholesterol, Lipolysis, Lipoprotein lipase, Lipoprotein metabolism",
author = "Taskinen, {Marja Riitta} and Kashyap, {Moti L.} and Srivastava, {Laxmi S.} and Muhammad Ashraf and {David Johnson}, J. and Gladys Perisutti and David Brady and Glueck, {Charles J.} and Jackson, {Richard L.}",
year = "1982",
month = "1",
day = "1",
doi = "10.1016/0021-9150(82)90202-7",
language = "English (US)",
volume = "41",
pages = "381--394",
journal = "Atherosclerosis",
issn = "0021-9150",
publisher = "Elsevier Ireland Ltd",
number = "2-3",

}

TY - JOUR

T1 - In vitro catabolism of human plasma very low density lipoproteins. Effects of VLDL concentration on the interconversion of high density lipoprotein subfractions

AU - Taskinen, Marja Riitta

AU - Kashyap, Moti L.

AU - Srivastava, Laxmi S.

AU - Ashraf, Muhammad

AU - David Johnson, J.

AU - Perisutti, Gladys

AU - Brady, David

AU - Glueck, Charles J.

AU - Jackson, Richard L.

PY - 1982/1/1

Y1 - 1982/1/1

N2 - The effect of lipolysis of human plasma very low density lipoproteins (VLDL) on the distribution of high density lipoprotein subfractions was studied in an in vitro system consisting of purified bovine milk lipoprotein lipase and albumin. The distribution of lipids and apoproteins (apoC-II and apoC-III) within the lipoprotein fractions corresponding to HDL2 (d = 1.063-1.120 g/ml) and HDL3 (d = 1.120-1.210 g/ml) was dependent upon the concentration of VLDL in the incubation mixture. After lipolysis of an incubation mixture containing VLDL-triglyceride (0.6 mg triglyceride/ml) and HDL3 (0.1 mg protein/ml), most of the lipid and apoproteins were recovered in HDL3. At higher concentrations of VLDL-triglyceride relative to HDL3-protein (1.8 or 2.4 mg of VLDL-triglyceride and 0.1 mg of HDL3-protein) the amount of lipid and apoprotein isolated in the HDL3 density fraction decreased after lipolysis and there was an increase in the amount isolated between d 1.063-1.120 g/ml. These results provide additional evidence for the conversion of HDL3 to HDL2 during lipolysis. Furthermore, they suggest that the relative distribution of plasma HDL2 and HDL3 is related to the rate of catabolism of triglyceride-rich lipoproteins.

AB - The effect of lipolysis of human plasma very low density lipoproteins (VLDL) on the distribution of high density lipoprotein subfractions was studied in an in vitro system consisting of purified bovine milk lipoprotein lipase and albumin. The distribution of lipids and apoproteins (apoC-II and apoC-III) within the lipoprotein fractions corresponding to HDL2 (d = 1.063-1.120 g/ml) and HDL3 (d = 1.120-1.210 g/ml) was dependent upon the concentration of VLDL in the incubation mixture. After lipolysis of an incubation mixture containing VLDL-triglyceride (0.6 mg triglyceride/ml) and HDL3 (0.1 mg protein/ml), most of the lipid and apoproteins were recovered in HDL3. At higher concentrations of VLDL-triglyceride relative to HDL3-protein (1.8 or 2.4 mg of VLDL-triglyceride and 0.1 mg of HDL3-protein) the amount of lipid and apoprotein isolated in the HDL3 density fraction decreased after lipolysis and there was an increase in the amount isolated between d 1.063-1.120 g/ml. These results provide additional evidence for the conversion of HDL3 to HDL2 during lipolysis. Furthermore, they suggest that the relative distribution of plasma HDL2 and HDL3 is related to the rate of catabolism of triglyceride-rich lipoproteins.

KW - HDL cholesterol

KW - Lipolysis

KW - Lipoprotein lipase

KW - Lipoprotein metabolism

UR - http://www.scopus.com/inward/record.url?scp=0020042953&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020042953&partnerID=8YFLogxK

U2 - 10.1016/0021-9150(82)90202-7

DO - 10.1016/0021-9150(82)90202-7

M3 - Article

C2 - 7066084

AN - SCOPUS:0020042953

VL - 41

SP - 381

EP - 394

JO - Atherosclerosis

JF - Atherosclerosis

SN - 0021-9150

IS - 2-3

ER -