TY - JOUR
T1 - In vitro corneal endothelial permeability in rabbit and human
T2 - The effects of age, cataract surgery and diabetes
AU - Watsky, Mitchell A.
AU - McDermott, Mark L.
AU - Edelhauser, Henry F.
N1 - Funding Information:
This work was supported by grants T32 EY07016, P31 EY01931, and R01 EY00933 from the National Institutes of Health and by Alcon Laboratories, Fort Worth, Texas. Dr Edelhauser is a Research to Prevent Blindness Senior Scientific Investigator.
PY - 1989/11
Y1 - 1989/11
N2 - Endothelial permeability was examined in rabbit and human corneas using an in vitro perfusion system with 5(6)-carboxyflurorescein as the permeability tracer. Following endothelial removal, the permeability of de-epithelialized rabbit corneas increased from 3·19×10-4 cm min-1 to 31·21×10-4 cm min-1, and de-epithelialized human donor corneal permeability values increased from 2·26 to 12·85. In human corneas, no correlation was seen between endothelial permeability and donor age, moist chamber storage time, endothelial cell density, coefficient of variation of cell area, or percent hexagonal cells. A positive correlation was found between permeability and time between donor death and enucleation. Donor eyes from three separate populations, having undergone cataract surgery prior to death (aphakic, posterior and anterior chamber implants), all showed a significant increase in permeability. Neither Type I nor Type II diabetes had an effect on the endothelial permeability of human donor corneas.
AB - Endothelial permeability was examined in rabbit and human corneas using an in vitro perfusion system with 5(6)-carboxyflurorescein as the permeability tracer. Following endothelial removal, the permeability of de-epithelialized rabbit corneas increased from 3·19×10-4 cm min-1 to 31·21×10-4 cm min-1, and de-epithelialized human donor corneal permeability values increased from 2·26 to 12·85. In human corneas, no correlation was seen between endothelial permeability and donor age, moist chamber storage time, endothelial cell density, coefficient of variation of cell area, or percent hexagonal cells. A positive correlation was found between permeability and time between donor death and enucleation. Donor eyes from three separate populations, having undergone cataract surgery prior to death (aphakic, posterior and anterior chamber implants), all showed a significant increase in permeability. Neither Type I nor Type II diabetes had an effect on the endothelial permeability of human donor corneas.
KW - carboxyfluorescein
KW - cataract surgery
KW - cornea
KW - diabetes
KW - endothelial permeability
KW - steady state distribution ratio (R)
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U2 - 10.1016/S0014-4835(89)80036-3
DO - 10.1016/S0014-4835(89)80036-3
M3 - Article
C2 - 2591492
AN - SCOPUS:0024332032
SN - 0014-4835
VL - 49
SP - 751
EP - 767
JO - Experimental Eye Research
JF - Experimental Eye Research
IS - 5
ER -