In Vitro Cytotoxicity Evaluation of a Self-adhesive, Methacrylate Resin-based Root Canal Sealer

Luigi Pinna, Martha G. Brackett, Petra E. Lockwood, Bradford P. Huffman, Sui Mai, Elisabetta Cotti, Claudia Dettori, David H. Pashley, Franklin R. Tay

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

This study compared the cytotoxicity of MetaSEAL (Parkell Inc, Farmington, NY), a methacrylate resin-based sealer with an epoxy resin-based (AH Plus Jet; Dentsply Caulk, Milford, DE) and a zinc oxide-eugenol-based sealer (Pulp Canal Sealer; SybronEndo, Orange, CA). Five-millimeter diameter disks prepared from the respective sealer and disks prepared from Teflon (negative control) and polymethyl methacrylate (positive control) were placed in direct contact with a rat osteosarcoma (ROS) 17/2.8 rat osteoblast-like cell line at six intervals after setting completely at 72 hours and for 5 succeeding weeks after the disks were immersed in simulated body fluid. Succinate dehydrogenase activity was evaluated by using 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide assay. All sealers exhibited severe toxicity at 72 hours, after which toxicity decreased gradually over the experimental period except for Pulp Canal Sealer, which remained severely toxic. MetaSEAL was more toxic than AH Plus Jet during the first week. Both were similar to the toxicity profile of the positive control after the first week, which was probably diffusion controlled.

Original languageEnglish (US)
Pages (from-to)1085-1088
Number of pages4
JournalJournal of Endodontics
Volume34
Issue number9
DOIs
StatePublished - Sep 1 2008

Fingerprint

Methacrylates
Poisons
Dental Pulp Cavity
Adhesives
Zinc Oxide
Epoxy Resins
Eugenol
Succinate Dehydrogenase
Polytetrafluoroethylene
Body Fluids
Polymethyl Methacrylate
Osteosarcoma
Osteoblasts
Bromides
Cell Line
epoxy resin-based root canal sealer
canals sealer
In Vitro Techniques
CMW cement
diphenyl

Keywords

  • 3-(4;5-dimethylthiazole-2-yl)-2;5-diphenyl tetrazolium bromide assay
  • Cell culture
  • MetaSEAL
  • cytoxicity
  • succinate dehydrogenase

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

In Vitro Cytotoxicity Evaluation of a Self-adhesive, Methacrylate Resin-based Root Canal Sealer. / Pinna, Luigi; Brackett, Martha G.; Lockwood, Petra E.; Huffman, Bradford P.; Mai, Sui; Cotti, Elisabetta; Dettori, Claudia; Pashley, David H.; Tay, Franklin R.

In: Journal of Endodontics, Vol. 34, No. 9, 01.09.2008, p. 1085-1088.

Research output: Contribution to journalArticle

Pinna, L, Brackett, MG, Lockwood, PE, Huffman, BP, Mai, S, Cotti, E, Dettori, C, Pashley, DH & Tay, FR 2008, 'In Vitro Cytotoxicity Evaluation of a Self-adhesive, Methacrylate Resin-based Root Canal Sealer', Journal of Endodontics, vol. 34, no. 9, pp. 1085-1088. https://doi.org/10.1016/j.joen.2008.06.020
Pinna, Luigi ; Brackett, Martha G. ; Lockwood, Petra E. ; Huffman, Bradford P. ; Mai, Sui ; Cotti, Elisabetta ; Dettori, Claudia ; Pashley, David H. ; Tay, Franklin R. / In Vitro Cytotoxicity Evaluation of a Self-adhesive, Methacrylate Resin-based Root Canal Sealer. In: Journal of Endodontics. 2008 ; Vol. 34, No. 9. pp. 1085-1088.
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