In vitro cytotoxicity of dental composites based on new and traditional polymerization chemistries

Martha G Brackett, S. Bouillaguet, P. E. Lockwood, S. Rotenberg, J. B. Lewis, Regina L W Messer, J. C. Wataha

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The biological response to dental restorative polymer composites is mediated by the release of unpolymerized residual monomers. Several new composite formulations claim to reduce unpolymerized residual mass. The current study assessed the cytotoxic responses to several of these new formations and compared them with more traditional formulations. Our hypothesis predicted that if these new polymerization chemistries reduce unpolymerized residual mass, the cytotoxicity of these materials also should be reduced relative to traditional formulations. Methods: Materials (HerculiteXRV, Premise, Filtek Supreme, CeramxDuo, Hermes, and Quixfil) were tested in vitro in direct contact with Balb mouse fibroblasts, initially, then after aging in artificial saliva for 0, 1, 3, 5, or 8 weeks. The toxicity was determined by using the MTT assay to the estimate SDH activity. Knoop hardness of the materials also was measured at 0 and 8 weeks to determine whether surface breakdown of the materials in artificial saliva contributed to cytotoxic responses. Results: Materials with traditional methacrylate chemistries (Herculite, Premise, Filtek Supreme) were severely (>50%) cytotoxic throughout the 8-week interval, but materials with newer chemistries or filling strategies (Hermes, CeramXDuo, and Quixfil) improved over time of aging in artificial saliva. Hermes showed the least cytotoxicity at 8 weeks, and was statistically equivalent to Teflon® negative controls. Hardness of the materials was unaffected by exposure to artificial saliva. Conclusions: Newer polymerization and filling strategies for dental composites show promise for reducing the release of unpolymerized components and cytotoxicity.

Original languageEnglish (US)
Pages (from-to)397-402
Number of pages6
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume81
Issue number2
DOIs
StatePublished - May 1 2007

Fingerprint

Dental composites
Artificial Saliva
Cytotoxicity
Polymerization
Tooth
Hardness
Methacrylates
Polytetrafluoroethylene
Aging of materials
Polymers
Fibroblasts
Composite materials
In Vitro Techniques
Polytetrafluoroethylenes
Toxicity
Assays
Monomers
QuiXfil
Filtek Supreme

Keywords

  • Cell-culture
  • Knoop hardness
  • Mitochondrial activity
  • Ormocer
  • Silorane

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

Cite this

In vitro cytotoxicity of dental composites based on new and traditional polymerization chemistries. / Brackett, Martha G; Bouillaguet, S.; Lockwood, P. E.; Rotenberg, S.; Lewis, J. B.; Messer, Regina L W; Wataha, J. C.

In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol. 81, No. 2, 01.05.2007, p. 397-402.

Research output: Contribution to journalArticle

@article{05349b0e01354b39a40e09880be41227,
title = "In vitro cytotoxicity of dental composites based on new and traditional polymerization chemistries",
abstract = "The biological response to dental restorative polymer composites is mediated by the release of unpolymerized residual monomers. Several new composite formulations claim to reduce unpolymerized residual mass. The current study assessed the cytotoxic responses to several of these new formations and compared them with more traditional formulations. Our hypothesis predicted that if these new polymerization chemistries reduce unpolymerized residual mass, the cytotoxicity of these materials also should be reduced relative to traditional formulations. Methods: Materials (HerculiteXRV, Premise, Filtek Supreme, CeramxDuo, Hermes, and Quixfil) were tested in vitro in direct contact with Balb mouse fibroblasts, initially, then after aging in artificial saliva for 0, 1, 3, 5, or 8 weeks. The toxicity was determined by using the MTT assay to the estimate SDH activity. Knoop hardness of the materials also was measured at 0 and 8 weeks to determine whether surface breakdown of the materials in artificial saliva contributed to cytotoxic responses. Results: Materials with traditional methacrylate chemistries (Herculite, Premise, Filtek Supreme) were severely (>50{\%}) cytotoxic throughout the 8-week interval, but materials with newer chemistries or filling strategies (Hermes, CeramXDuo, and Quixfil) improved over time of aging in artificial saliva. Hermes showed the least cytotoxicity at 8 weeks, and was statistically equivalent to Teflon{\circledR} negative controls. Hardness of the materials was unaffected by exposure to artificial saliva. Conclusions: Newer polymerization and filling strategies for dental composites show promise for reducing the release of unpolymerized components and cytotoxicity.",
keywords = "Cell-culture, Knoop hardness, Mitochondrial activity, Ormocer, Silorane",
author = "Brackett, {Martha G} and S. Bouillaguet and Lockwood, {P. E.} and S. Rotenberg and Lewis, {J. B.} and Messer, {Regina L W} and Wataha, {J. C.}",
year = "2007",
month = "5",
day = "1",
doi = "10.1002/jbm.b.30676",
language = "English (US)",
volume = "81",
pages = "397--402",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "0021-9304",
publisher = "Heterocorporation",
number = "2",

}

TY - JOUR

T1 - In vitro cytotoxicity of dental composites based on new and traditional polymerization chemistries

AU - Brackett, Martha G

AU - Bouillaguet, S.

AU - Lockwood, P. E.

AU - Rotenberg, S.

AU - Lewis, J. B.

AU - Messer, Regina L W

AU - Wataha, J. C.

PY - 2007/5/1

Y1 - 2007/5/1

N2 - The biological response to dental restorative polymer composites is mediated by the release of unpolymerized residual monomers. Several new composite formulations claim to reduce unpolymerized residual mass. The current study assessed the cytotoxic responses to several of these new formations and compared them with more traditional formulations. Our hypothesis predicted that if these new polymerization chemistries reduce unpolymerized residual mass, the cytotoxicity of these materials also should be reduced relative to traditional formulations. Methods: Materials (HerculiteXRV, Premise, Filtek Supreme, CeramxDuo, Hermes, and Quixfil) were tested in vitro in direct contact with Balb mouse fibroblasts, initially, then after aging in artificial saliva for 0, 1, 3, 5, or 8 weeks. The toxicity was determined by using the MTT assay to the estimate SDH activity. Knoop hardness of the materials also was measured at 0 and 8 weeks to determine whether surface breakdown of the materials in artificial saliva contributed to cytotoxic responses. Results: Materials with traditional methacrylate chemistries (Herculite, Premise, Filtek Supreme) were severely (>50%) cytotoxic throughout the 8-week interval, but materials with newer chemistries or filling strategies (Hermes, CeramXDuo, and Quixfil) improved over time of aging in artificial saliva. Hermes showed the least cytotoxicity at 8 weeks, and was statistically equivalent to Teflon® negative controls. Hardness of the materials was unaffected by exposure to artificial saliva. Conclusions: Newer polymerization and filling strategies for dental composites show promise for reducing the release of unpolymerized components and cytotoxicity.

AB - The biological response to dental restorative polymer composites is mediated by the release of unpolymerized residual monomers. Several new composite formulations claim to reduce unpolymerized residual mass. The current study assessed the cytotoxic responses to several of these new formations and compared them with more traditional formulations. Our hypothesis predicted that if these new polymerization chemistries reduce unpolymerized residual mass, the cytotoxicity of these materials also should be reduced relative to traditional formulations. Methods: Materials (HerculiteXRV, Premise, Filtek Supreme, CeramxDuo, Hermes, and Quixfil) were tested in vitro in direct contact with Balb mouse fibroblasts, initially, then after aging in artificial saliva for 0, 1, 3, 5, or 8 weeks. The toxicity was determined by using the MTT assay to the estimate SDH activity. Knoop hardness of the materials also was measured at 0 and 8 weeks to determine whether surface breakdown of the materials in artificial saliva contributed to cytotoxic responses. Results: Materials with traditional methacrylate chemistries (Herculite, Premise, Filtek Supreme) were severely (>50%) cytotoxic throughout the 8-week interval, but materials with newer chemistries or filling strategies (Hermes, CeramXDuo, and Quixfil) improved over time of aging in artificial saliva. Hermes showed the least cytotoxicity at 8 weeks, and was statistically equivalent to Teflon® negative controls. Hardness of the materials was unaffected by exposure to artificial saliva. Conclusions: Newer polymerization and filling strategies for dental composites show promise for reducing the release of unpolymerized components and cytotoxicity.

KW - Cell-culture

KW - Knoop hardness

KW - Mitochondrial activity

KW - Ormocer

KW - Silorane

UR - http://www.scopus.com/inward/record.url?scp=34247610806&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34247610806&partnerID=8YFLogxK

U2 - 10.1002/jbm.b.30676

DO - 10.1002/jbm.b.30676

M3 - Article

C2 - 17022055

AN - SCOPUS:34247610806

VL - 81

SP - 397

EP - 402

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 0021-9304

IS - 2

ER -