In Vitro Effect of Vancomycin on the Viability of Articular Chondrocytes

K. Aaron Shaw, Josef K. Eichinger, Nischal Nadig, Stephen Arthur Parada

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Objectives: To characterize the in vitro toxicity of vancomycin on articular cartilage. Methods: Osteochondral samples extracted from fresh, juvenile porcine stifle (knee) joints were exposed to vancomycin saline concentrations of 2, 5, and 10 mg/mL and compared with a saline and nonsaline treated control. Comparison of chondrocyte viability was assessed with histology examination of chondrocyte degeneration using the Mankin criteria and a live/dead staining using ethidium homodimer-2 and calcien acetoxymethylester stain and confocal laser scanning microscopy. Results: A comparison of chondrocyte viability in the control sample and 2 mg/mL group showed no statistical difference (P = 0.38). The chondrocyte toxicity was statistically significantly higher in both the 5 mg/mL (P = 0.003) and 10 mg/mL (P < 0.001) experimental groups in comparison to the control sample. Chondrocyte death increased in a statistically significant dose-dependent fashion. Histologically, the Mankin scores were higher for the saline-treated group in comparison to the untreated control group. Conclusions: Vancomycin is toxic to articular chondrocytes in concentrations of 5 mg/mL and greater. Strategies for controlling concentration must be developed before routine application of topical antibiotics around synovial joints is performed.

Original languageEnglish (US)
Pages (from-to)148-153
Number of pages6
JournalJournal of Orthopaedic Trauma
Volume32
Issue number3
DOIs
StatePublished - Mar 1 2018

Keywords

  • articular chondrocytes
  • porcine model
  • vancomycin

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine

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