In vitro effects of elastase on rabbit craniomandibular joint articular disks

N. L. O'Dell, M. Sharawy, B. C. Starcher, C. B. Pennington, R. P. Cudia

Research output: Contribution to journalArticle

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Abstract

This in vitro study correlates morphologic and radioimmunoassay (RIA) find-ings on the effects of elastase on the elastic fibers that are found in the rabbit craniomandibular joint (CMJ) articular disk. Articular disks were removed from rabbit CMJs at necropsy, and cut sagittally into two pieces which were incubated in 0.3 ml of phosphate-buffered saline containing either 0, 12.5, 25 or 50 units of porcine pancreatic elastase for either 1, 3 or 24 h. The quantitative RIA findings correlated well with the qualitative light-microscopic observations in that both methods showed a reduction in the amounts of elastin in the CMJ disks following enzyme treatment. However, the morphologic appearance of most of the elastase-treated disks suggested that the destruction of the elastic fibers was more extensive than was suggested by the results of the RIA which indicated that some elastin remained in the tissues of the disks even when the highest enzyme level and longest incubation period were combined. The results of this study also support the interpretation that the resorcin-fuchsin-stained fibers in the rabbit CMJ disk are elastic fibers.

Original languageEnglish (US)
Pages (from-to)229-233
Number of pages5
JournalActa Anatomica
Volume145
Issue number3
StatePublished - Jan 1 1992

Fingerprint

Pancreatic Elastase
Joints
Elastic Tissue
Rabbits
Radioimmunoassay
Elastin
Rosaniline Dyes
Enzymes
Swine
Phosphates
In Vitro Techniques
Light

Keywords

  • Articular disk
  • Craniomandibular joint
  • Elastase
  • Elastic fibers
  • Rabbit

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

O'Dell, N. L., Sharawy, M., Starcher, B. C., Pennington, C. B., & Cudia, R. P. (1992). In vitro effects of elastase on rabbit craniomandibular joint articular disks. Acta Anatomica, 145(3), 229-233.

In vitro effects of elastase on rabbit craniomandibular joint articular disks. / O'Dell, N. L.; Sharawy, M.; Starcher, B. C.; Pennington, C. B.; Cudia, R. P.

In: Acta Anatomica, Vol. 145, No. 3, 01.01.1992, p. 229-233.

Research output: Contribution to journalArticle

O'Dell, NL, Sharawy, M, Starcher, BC, Pennington, CB & Cudia, RP 1992, 'In vitro effects of elastase on rabbit craniomandibular joint articular disks', Acta Anatomica, vol. 145, no. 3, pp. 229-233.
O'Dell NL, Sharawy M, Starcher BC, Pennington CB, Cudia RP. In vitro effects of elastase on rabbit craniomandibular joint articular disks. Acta Anatomica. 1992 Jan 1;145(3):229-233.
O'Dell, N. L. ; Sharawy, M. ; Starcher, B. C. ; Pennington, C. B. ; Cudia, R. P. / In vitro effects of elastase on rabbit craniomandibular joint articular disks. In: Acta Anatomica. 1992 ; Vol. 145, No. 3. pp. 229-233.
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