In vitro functional capabilities of canine polymorphonuclear neutrophils collected simultaneously by continuous‐flow centrifugation and continuous‐flow filtration leukopheresis

Lloyd O. Cook, Laurence A. Boxer, John M. Allen, John A. Griep, Robert L. Baehner

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Polymorphonuclear neutrophils were simultaneously collected from dogs by continuous‐flow centrifugation and continuous‐flow filtration leukapheresis. In vitro studies were performed on cells obtained by the two methods as well as on control cells. Studies consisted of assessment of phagocytic capacity, degranulation, chemotaxis, hexose monophosphate (HMP) shunt activity, and bacterial killing. The cells obtained from the filter were metabolically more active than those harvested by centrifugation, as evidenced by increase in resting HMP shunt activity and dimunition in total available lysozyme‐secreting activity compared to centrifuged cells. Despite their impaired phagocytic capacities, the filtered cells were able to kill Staphylococcus aureus as efficiently as the centrifuged cells. Both cell populations responded to chemotactic gradients equally.

Original languageEnglish (US)
Pages (from-to)225-232
Number of pages8
JournalAmerican Journal of Hematology
Volume4
Issue number3
DOIs
StatePublished - 1978

Keywords

  • centrifuge
  • filter
  • granulocyte function

ASJC Scopus subject areas

  • Hematology

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