In vitro immunization and expansion of antigen-specific cytotoxic T lymphocytes for adoptive immunotherapy using peptide-pulsed dendritic cells

Van Tsai, Ichiro Kawashima, Elissa Keogh, Kieran Daly, Alessandro Sette, Esteban Celis

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

The design of an effective procedure to sensitize and expand antigen- specific cytotoxic T lymphocytes (CTL) in vitro is essential for the development of effective adoptive cellular immunotherapy protocols for cancer. We have analyzed the capacity of tissue culture-derived dendritic cells (DC) to present specific peptide epitopes to CTL precursors. Our results demonstrate that peptide-pulsed DC were efficient in generating CTL responses specific for various vital and tumor epitopes. Furthermore, IL-7 and IL-10 potentiated the ability of the peptide-pulsed DC to trigger antigen-specific CTL responses. The CTL generated using this procedure efficiently recognized the naturally processed antigens and could be expanded approximately 100- to 1000-fold in tissue culture in 10 to 15 days without a loss of activity and specificity. The results and procedures described herein may facilitate the development of effective CTL-based adoptive immunotherapy for chronic vital diseases and cancer.

Original languageEnglish (US)
Pages (from-to)65-75
Number of pages11
JournalCritical Reviews in Immunology
Volume18
Issue number1-2
StatePublished - Jan 1 1997
Externally publishedYes

Keywords

  • CTL
  • Dendritic cells
  • MHC binding peptides
  • Tumor antigens

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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