In vitro Inactivation and Stabilization of Fibrinolytic Activity of Urokinase

R. B. Aisina, L. I. Mukhametova, Yu I. Sazonova, S. D. Varfolomeev

Research output: Contribution to journalArticle

Abstract

When incubated at 30°C and pH 7.4, urokinase lost fibrinolytic activity (i.e., the plasminogen-activating activity measured by the time of fibrin clot lysis) but completely retained amidase activity. The enzyme inactivation rate depended on the urokinase concentration and, at concentrations of more than 1.5 μM, was described by a second order equation, which indicated that the enzyme underwent autolytic degradation (kaut = 3.8 × 10-3 M-1 min-1). During incubation, urokinase (54 kDa) was converted into its low-molecular-mass form (33 kDa) and products of the A-chain degradation. The amidase activity did not correlate with the fibrinolytic activity in the cases when the enzyme molecule underwent local unfolding or partial degradation. The optimum mixture of agents for stabilizing the fibrinolytic activity of urokinase was found.

Original languageEnglish (US)
Number of pages1
JournalBioorganicheskaya Khimiya
Volume24
Issue number7
StatePublished - Dec 1 1998

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Urokinase-Type Plasminogen Activator
amidase
Stabilization
Degradation
Enzymes
Fibrin Clot Lysis Time
Fibrinolytic Agents
Excipients
Plasminogen
Molecular mass
Fibrin
Molecules
In Vitro Techniques

Keywords

  • Amidase and fibrinolytic activities
  • Autolysis
  • Inactivation kinetics
  • Stabilization
  • Urokinase

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Organic Chemistry

Cite this

Aisina, R. B., Mukhametova, L. I., Sazonova, Y. I., & Varfolomeev, S. D. (1998). In vitro Inactivation and Stabilization of Fibrinolytic Activity of Urokinase. Bioorganicheskaya Khimiya, 24(7).

In vitro Inactivation and Stabilization of Fibrinolytic Activity of Urokinase. / Aisina, R. B.; Mukhametova, L. I.; Sazonova, Yu I.; Varfolomeev, S. D.

In: Bioorganicheskaya Khimiya, Vol. 24, No. 7, 01.12.1998.

Research output: Contribution to journalArticle

Aisina, RB, Mukhametova, LI, Sazonova, YI & Varfolomeev, SD 1998, 'In vitro Inactivation and Stabilization of Fibrinolytic Activity of Urokinase', Bioorganicheskaya Khimiya, vol. 24, no. 7.
Aisina RB, Mukhametova LI, Sazonova YI, Varfolomeev SD. In vitro Inactivation and Stabilization of Fibrinolytic Activity of Urokinase. Bioorganicheskaya Khimiya. 1998 Dec 1;24(7).
Aisina, R. B. ; Mukhametova, L. I. ; Sazonova, Yu I. ; Varfolomeev, S. D. / In vitro Inactivation and Stabilization of Fibrinolytic Activity of Urokinase. In: Bioorganicheskaya Khimiya. 1998 ; Vol. 24, No. 7.
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