In vitro osteogenic/dentinogenic potential of an experimental calcium aluminosilicate cement

Ashraf A. Eid, Li Na Niu, Carolyn M. Primus, Lynne A. Opperman, David H. Pashley, Ikuya Watanabe, Franklin R. Tay

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Introduction: Calcium aluminosilicate cements are fast-setting, acid-resistant, bioactive cements that may be used as root-repair materials. This study examined the osteogenic/dentinogenic potential of an experimental calcium aluminosilicate cement (Quick-Set) by using a murine odontoblast-like cell model. Methods: Quick-Set and white ProRoot MTA (WMTA) were mixed with the proprietary gel or deionized water, allowed to set completely in 100% relative humidity, and aged in complete growth medium for 2 weeks until rendered non-cytotoxic. Similarly aged Teflon disks were used as negative control. The MDPC-23 cell line was used for evaluating changes in mRNA expressions of genes associated with osteogenic/dentinogenic differentiation and mineralization (quantitative reverse transcription polymerase chain reaction), alkaline phosphatase enzyme production, and extracellular matrix mineralization (alizarin red S staining). Results: After MDPC-23 cells were incubated with the materials in osteogenic differentiation medium for 1 week, both cements showed up-regulation in ALP and DSPP expression. Fold increases in these 2 genes were not significantly different between Quick-Set and WMTA. Both cements showed no statistically significant up-regulation/down-regulation in RUNX2, OCN, BSP, and DMP1 gene expression compared with Teflon. Alkaline phosphatase activity of cells cultured on Quick-Set and WMTA were not significantly different at 1 week or 2 weeks but were significantly higher (P <.05) than Teflon in both weeks. Both cements showed significantly higher calcium deposition compared with Teflon after 3 weeks of incubation in mineralizing medium (P <.001). Differences between Quick-Set and WMTA were not statistically significant. Conclusions: The experimental calcium aluminosilicate cement exhibits similar osteogenic/dentinogenic properties to WMTA and may be a potential substitute for commercially available tricalcium silicate cements.

Original languageEnglish (US)
Pages (from-to)1161-1166
Number of pages6
JournalJournal of Endodontics
Volume39
Issue number9
DOIs
StatePublished - Sep 1 2013

Keywords

  • Alizarin red S staining
  • MDPC-23 cells
  • alkaline phosphatase
  • calcium aluminosilicate cement
  • quantitative reverse transcription polymerase chain reaction

ASJC Scopus subject areas

  • Dentistry(all)

Fingerprint Dive into the research topics of 'In vitro osteogenic/dentinogenic potential of an experimental calcium aluminosilicate cement'. Together they form a unique fingerprint.

  • Cite this

    Eid, A. A., Niu, L. N., Primus, C. M., Opperman, L. A., Pashley, D. H., Watanabe, I., & Tay, F. R. (2013). In vitro osteogenic/dentinogenic potential of an experimental calcium aluminosilicate cement. Journal of Endodontics, 39(9), 1161-1166. https://doi.org/10.1016/j.joen.2013.04.005