In vitro osteogenic/dentinogenic potential of an experimental calcium aluminosilicate cement

Ashraf A. Eid, Li Na Niu, Carolyn M. Primus, Lynne A. Opperman, David H. Pashley, Ikuya Watanabe, Franklin R. Tay

Research output: Contribution to journalArticle

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Abstract

Introduction: Calcium aluminosilicate cements are fast-setting, acid-resistant, bioactive cements that may be used as root-repair materials. This study examined the osteogenic/dentinogenic potential of an experimental calcium aluminosilicate cement (Quick-Set) by using a murine odontoblast-like cell model. Methods: Quick-Set and white ProRoot MTA (WMTA) were mixed with the proprietary gel or deionized water, allowed to set completely in 100% relative humidity, and aged in complete growth medium for 2 weeks until rendered non-cytotoxic. Similarly aged Teflon disks were used as negative control. The MDPC-23 cell line was used for evaluating changes in mRNA expressions of genes associated with osteogenic/dentinogenic differentiation and mineralization (quantitative reverse transcription polymerase chain reaction), alkaline phosphatase enzyme production, and extracellular matrix mineralization (alizarin red S staining). Results: After MDPC-23 cells were incubated with the materials in osteogenic differentiation medium for 1 week, both cements showed up-regulation in ALP and DSPP expression. Fold increases in these 2 genes were not significantly different between Quick-Set and WMTA. Both cements showed no statistically significant up-regulation/down-regulation in RUNX2, OCN, BSP, and DMP1 gene expression compared with Teflon. Alkaline phosphatase activity of cells cultured on Quick-Set and WMTA were not significantly different at 1 week or 2 weeks but were significantly higher (P <.05) than Teflon in both weeks. Both cements showed significantly higher calcium deposition compared with Teflon after 3 weeks of incubation in mineralizing medium (P <.001). Differences between Quick-Set and WMTA were not statistically significant. Conclusions: The experimental calcium aluminosilicate cement exhibits similar osteogenic/dentinogenic properties to WMTA and may be a potential substitute for commercially available tricalcium silicate cements.

Original languageEnglish (US)
Pages (from-to)1161-1166
Number of pages6
JournalJournal of Endodontics
Volume39
Issue number9
DOIs
StatePublished - Sep 1 2013

Fingerprint

Pemetrexed
Polytetrafluoroethylene
Alkaline Phosphatase
Silicate Cement
Up-Regulation
Odontoblasts
Gene Expression
Humidity
Reverse Transcription
Extracellular Matrix
Cultured Cells
Down-Regulation
Gels
calcium aluminosilicate
In Vitro Techniques
Staining and Labeling
Calcium
Cell Line
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Alizarin red S staining
  • MDPC-23 cells
  • alkaline phosphatase
  • calcium aluminosilicate cement
  • quantitative reverse transcription polymerase chain reaction

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Eid, A. A., Niu, L. N., Primus, C. M., Opperman, L. A., Pashley, D. H., Watanabe, I., & Tay, F. R. (2013). In vitro osteogenic/dentinogenic potential of an experimental calcium aluminosilicate cement. Journal of Endodontics, 39(9), 1161-1166. https://doi.org/10.1016/j.joen.2013.04.005

In vitro osteogenic/dentinogenic potential of an experimental calcium aluminosilicate cement. / Eid, Ashraf A.; Niu, Li Na; Primus, Carolyn M.; Opperman, Lynne A.; Pashley, David H.; Watanabe, Ikuya; Tay, Franklin R.

In: Journal of Endodontics, Vol. 39, No. 9, 01.09.2013, p. 1161-1166.

Research output: Contribution to journalArticle

Eid, AA, Niu, LN, Primus, CM, Opperman, LA, Pashley, DH, Watanabe, I & Tay, FR 2013, 'In vitro osteogenic/dentinogenic potential of an experimental calcium aluminosilicate cement', Journal of Endodontics, vol. 39, no. 9, pp. 1161-1166. https://doi.org/10.1016/j.joen.2013.04.005
Eid, Ashraf A. ; Niu, Li Na ; Primus, Carolyn M. ; Opperman, Lynne A. ; Pashley, David H. ; Watanabe, Ikuya ; Tay, Franklin R. / In vitro osteogenic/dentinogenic potential of an experimental calcium aluminosilicate cement. In: Journal of Endodontics. 2013 ; Vol. 39, No. 9. pp. 1161-1166.
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AU - Pashley, David H.

AU - Watanabe, Ikuya

AU - Tay, Franklin R.

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N2 - Introduction: Calcium aluminosilicate cements are fast-setting, acid-resistant, bioactive cements that may be used as root-repair materials. This study examined the osteogenic/dentinogenic potential of an experimental calcium aluminosilicate cement (Quick-Set) by using a murine odontoblast-like cell model. Methods: Quick-Set and white ProRoot MTA (WMTA) were mixed with the proprietary gel or deionized water, allowed to set completely in 100% relative humidity, and aged in complete growth medium for 2 weeks until rendered non-cytotoxic. Similarly aged Teflon disks were used as negative control. The MDPC-23 cell line was used for evaluating changes in mRNA expressions of genes associated with osteogenic/dentinogenic differentiation and mineralization (quantitative reverse transcription polymerase chain reaction), alkaline phosphatase enzyme production, and extracellular matrix mineralization (alizarin red S staining). Results: After MDPC-23 cells were incubated with the materials in osteogenic differentiation medium for 1 week, both cements showed up-regulation in ALP and DSPP expression. Fold increases in these 2 genes were not significantly different between Quick-Set and WMTA. Both cements showed no statistically significant up-regulation/down-regulation in RUNX2, OCN, BSP, and DMP1 gene expression compared with Teflon. Alkaline phosphatase activity of cells cultured on Quick-Set and WMTA were not significantly different at 1 week or 2 weeks but were significantly higher (P <.05) than Teflon in both weeks. Both cements showed significantly higher calcium deposition compared with Teflon after 3 weeks of incubation in mineralizing medium (P <.001). Differences between Quick-Set and WMTA were not statistically significant. Conclusions: The experimental calcium aluminosilicate cement exhibits similar osteogenic/dentinogenic properties to WMTA and may be a potential substitute for commercially available tricalcium silicate cements.

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