TY - JOUR
T1 - In vitro susceptibilities of Aspergillus species to voriconazole, itraconazole, and amphotericin B
AU - Abraham, O. C.
AU - Manavathu, Elias K.
AU - Cutright, Jessica L.
AU - Chandrasekar, Pranatharthi H.
N1 - Funding Information:
The authors wish to thank William Brown, Ph.D., Microbiology Laboratory, Detroit Medical Center for kindly providing the clinical isolates of various Aspergillus species used in this study. This study was supported by a grant from Pfizer Pharmaceuticals.
PY - 1999/1
Y1 - 1999/1
N2 - We studied the in vitro activity of voriconazole (VCZ) itraconazole (ITZ) and amphotericin B (AMB) against 216 clinical isolates of Aspergillus spp. (142 Aspergillus fumigatus and 74 nonfumigatus Aspergillus spp. isolates) using a broth macrodilution method. The MICs (μg/mL) (mean, range) for A. fumigatus were: VCZ 0.88, 0.25-4; ITZ 0.54, 0.25-4; AMB 2.16, 0.5-8. MIC90s were: VCZ 2, ITZ 1, AMB 4. MICs for nonfumigatus Aspergillus spp. were: VCZ 1.57, 0.25-4; ITZ 1.74, 0.25-4; AMB 2.88, 0.5-8. MIC90s for this group were: VCZ 4, ITZ 4, AMB 4. We also studied the susceptibility to VCZ of 18 AMB-resistant (mean, MIC 6.0 μg/mL) and 28 ITZ-resistant (mean, MIC 13.28 μg/mL) A. fumigatus isolates selected in the laboratory. The mean MICs of VCZ were 0.59 μg/mL for AMB-resistant and 1.32 μg/mL for ITZ-resistant isolates. Our study showed that VCZ and ITZ had comparable in vitro activity against the isolates studied, except against A. fumigatus, where the MIC of ITZ was lower. The azoles had better in vitro activity than AMB against A. fumigatus and non-fumigatus spp. The non-fumigatus Aspergillus spp. were less susceptible to all three antifungals evaluated. When tested against ITZ- or AMB-resistant A. fumigatus strains, VCZ retained good activity, showing only a modest rise in the MIC against ITZ-resistant strains. Copyright (C) 1999 Elsevier Science Inc.
AB - We studied the in vitro activity of voriconazole (VCZ) itraconazole (ITZ) and amphotericin B (AMB) against 216 clinical isolates of Aspergillus spp. (142 Aspergillus fumigatus and 74 nonfumigatus Aspergillus spp. isolates) using a broth macrodilution method. The MICs (μg/mL) (mean, range) for A. fumigatus were: VCZ 0.88, 0.25-4; ITZ 0.54, 0.25-4; AMB 2.16, 0.5-8. MIC90s were: VCZ 2, ITZ 1, AMB 4. MICs for nonfumigatus Aspergillus spp. were: VCZ 1.57, 0.25-4; ITZ 1.74, 0.25-4; AMB 2.88, 0.5-8. MIC90s for this group were: VCZ 4, ITZ 4, AMB 4. We also studied the susceptibility to VCZ of 18 AMB-resistant (mean, MIC 6.0 μg/mL) and 28 ITZ-resistant (mean, MIC 13.28 μg/mL) A. fumigatus isolates selected in the laboratory. The mean MICs of VCZ were 0.59 μg/mL for AMB-resistant and 1.32 μg/mL for ITZ-resistant isolates. Our study showed that VCZ and ITZ had comparable in vitro activity against the isolates studied, except against A. fumigatus, where the MIC of ITZ was lower. The azoles had better in vitro activity than AMB against A. fumigatus and non-fumigatus spp. The non-fumigatus Aspergillus spp. were less susceptible to all three antifungals evaluated. When tested against ITZ- or AMB-resistant A. fumigatus strains, VCZ retained good activity, showing only a modest rise in the MIC against ITZ-resistant strains. Copyright (C) 1999 Elsevier Science Inc.
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U2 - 10.1016/S0732-8893(98)00102-3
DO - 10.1016/S0732-8893(98)00102-3
M3 - Article
C2 - 9990469
AN - SCOPUS:0033011505
SN - 0732-8893
VL - 33
SP - 7
EP - 11
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 1
ER -
