In vitro translation of rat lung surfactant apoprotein mRNA

Sikandar L. Katyal, Gurmukh Singh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Rat pulmonary surfactant contains apoproteins of molecular weights 38,000, 32,000, 26,000 and 10,000-12,000. The structural and metabolic interrelationships of these proteins are not clear as yet. In order to investigate if they arise from a single or multiple precursor protein(s), we isolated total poly(A)RNA from rat lungs, performed its translation in vitro in the presence of [35s]-methionine and reticulocyte lysate, immunoprecipitated the translation products with anti-rat surfactant antibody, and analyzed them by SDS-polyacrylamide gel electrophoresis and autoradiography. A single translation product of molecular weight 35,000 was detected. Since the antibody used in the immunoprecipitation recognizes the 38,000, 32,000 and 26,000 dalton proteins, it is concluded that at least these three proteins arise from the 35,000 dalton precursor by post-translational modifications.

Original languageEnglish (US)
Pages (from-to)106-111
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume127
Issue number1
DOIs
StatePublished - Feb 28 1985

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Apoproteins
Surface-Active Agents
Rats
Lung
Messenger RNA
Molecular Weight
Molecular weight
Pulmonary Surfactants
Proteins
Protein Precursors
Antibodies
Reticulocytes
Post Translational Protein Processing
Electrophoresis
Autoradiography
Immunoprecipitation
Methionine
Polyacrylamide Gel Electrophoresis
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

In vitro translation of rat lung surfactant apoprotein mRNA. / Katyal, Sikandar L.; Singh, Gurmukh.

In: Biochemical and Biophysical Research Communications, Vol. 127, No. 1, 28.02.1985, p. 106-111.

Research output: Contribution to journalArticle

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