Abstract
Rat pulmonary surfactant contains apoproteins of molecular weights 38,000, 32,000, 26,000 and 10,000-12,000. The structural and metabolic interrelationships of these proteins are not clear as yet. In order to investigate if they arise from a single or multiple precursor protein(s), we isolated total poly(A)RNA from rat lungs, performed its translation in vitro in the presence of [35s]-methionine and reticulocyte lysate, immunoprecipitated the translation products with anti-rat surfactant antibody, and analyzed them by SDS-polyacrylamide gel electrophoresis and autoradiography. A single translation product of molecular weight 35,000 was detected. Since the antibody used in the immunoprecipitation recognizes the 38,000, 32,000 and 26,000 dalton proteins, it is concluded that at least these three proteins arise from the 35,000 dalton precursor by post-translational modifications.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 106-111 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 127 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 28 1985 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
In vitro translation of rat lung surfactant apoprotein mRNA. / Katyal, Sikandar L.; Singh, Gurmukh.
In: Biochemical and Biophysical Research Communications, Vol. 127, No. 1, 28.02.1985, p. 106-111.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro translation of rat lung surfactant apoprotein mRNA
AU - Katyal, Sikandar L.
AU - Singh, Gurmukh
PY - 1985/2/28
Y1 - 1985/2/28
N2 - Rat pulmonary surfactant contains apoproteins of molecular weights 38,000, 32,000, 26,000 and 10,000-12,000. The structural and metabolic interrelationships of these proteins are not clear as yet. In order to investigate if they arise from a single or multiple precursor protein(s), we isolated total poly(A)RNA from rat lungs, performed its translation in vitro in the presence of [35s]-methionine and reticulocyte lysate, immunoprecipitated the translation products with anti-rat surfactant antibody, and analyzed them by SDS-polyacrylamide gel electrophoresis and autoradiography. A single translation product of molecular weight 35,000 was detected. Since the antibody used in the immunoprecipitation recognizes the 38,000, 32,000 and 26,000 dalton proteins, it is concluded that at least these three proteins arise from the 35,000 dalton precursor by post-translational modifications.
AB - Rat pulmonary surfactant contains apoproteins of molecular weights 38,000, 32,000, 26,000 and 10,000-12,000. The structural and metabolic interrelationships of these proteins are not clear as yet. In order to investigate if they arise from a single or multiple precursor protein(s), we isolated total poly(A)RNA from rat lungs, performed its translation in vitro in the presence of [35s]-methionine and reticulocyte lysate, immunoprecipitated the translation products with anti-rat surfactant antibody, and analyzed them by SDS-polyacrylamide gel electrophoresis and autoradiography. A single translation product of molecular weight 35,000 was detected. Since the antibody used in the immunoprecipitation recognizes the 38,000, 32,000 and 26,000 dalton proteins, it is concluded that at least these three proteins arise from the 35,000 dalton precursor by post-translational modifications.
UR - http://www.scopus.com/inward/record.url?scp=0021947943&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021947943&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(85)80132-7
DO - 10.1016/S0006-291X(85)80132-7
M3 - Article
C2 - 3884005
AN - SCOPUS:0021947943
VL - 127
SP - 106
EP - 111
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -