In vivo diuretic actions of renal vasopressin V1 receptor stimulation in rats

C. Ledderhos, D. L. Mattson, M. M. Skelton, A. W. Cowley

Research output: Contribution to journalArticle

Abstract

The specific vasopressin V1 receptor agonist (V1AG; [Phe2,Ile3,Orn8]vasopressin) was infused (2.0 ng · kg-1 · mi-1) into the renal medullary interstitial space to determine the effects of selective medullary V1 receptor stimulation on sodium and water excretion in normal rats. Responses were compared with those of arginine vasopressin (AVP) and vasopressin V2 receptor stimulation resulting from infusion of a V1 receptor antagonist with AVP. Medullary infusion of V1AG or AVP in euvolemic rats produced no changes in hemodynamics or glomerular filtration rate. V1AG increased urine flow > 60% in euvolemic rats, whereas no change was observed with AVP. This response could not be explained by a rise of arterial pressure or by volume retention. With V2 stimulation in euvolemic rats, urine flow was decreased. In water diuretic rats, V1AG produced no change, whereas AVP infusion decreased urine flow. The results provide in vivo evidence that tubular V1 vasopressin receptor activity results in increased urine flow and thereby modulates the antidiuretic actions of vasopressin in the euvolemic state.

Original languageEnglish (US)
Pages (from-to)R796-R807
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume268
Issue number3 37-3
StatePublished - Jan 1 1995
Externally publishedYes

Fingerprint

Vasopressin Receptors
Diuretics
Arginine Vasopressin
Kidney
Urine
Vasopressins
Water
Glomerular Filtration Rate
Arterial Pressure
Hemodynamics
Sodium

Keywords

  • diuresis
  • natriuresis
  • rat
  • renal medulla

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

In vivo diuretic actions of renal vasopressin V1 receptor stimulation in rats. / Ledderhos, C.; Mattson, D. L.; Skelton, M. M.; Cowley, A. W.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 268, No. 3 37-3, 01.01.1995, p. R796-R807.

Research output: Contribution to journalArticle

Ledderhos, C. ; Mattson, D. L. ; Skelton, M. M. ; Cowley, A. W. / In vivo diuretic actions of renal vasopressin V1 receptor stimulation in rats. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 1995 ; Vol. 268, No. 3 37-3. pp. R796-R807.
@article{bbcf26271fc04c83b2faa79cb20b5a57,
title = "In vivo diuretic actions of renal vasopressin V1 receptor stimulation in rats",
abstract = "The specific vasopressin V1 receptor agonist (V1AG; [Phe2,Ile3,Orn8]vasopressin) was infused (2.0 ng · kg-1 · mi-1) into the renal medullary interstitial space to determine the effects of selective medullary V1 receptor stimulation on sodium and water excretion in normal rats. Responses were compared with those of arginine vasopressin (AVP) and vasopressin V2 receptor stimulation resulting from infusion of a V1 receptor antagonist with AVP. Medullary infusion of V1AG or AVP in euvolemic rats produced no changes in hemodynamics or glomerular filtration rate. V1AG increased urine flow > 60{\%} in euvolemic rats, whereas no change was observed with AVP. This response could not be explained by a rise of arterial pressure or by volume retention. With V2 stimulation in euvolemic rats, urine flow was decreased. In water diuretic rats, V1AG produced no change, whereas AVP infusion decreased urine flow. The results provide in vivo evidence that tubular V1 vasopressin receptor activity results in increased urine flow and thereby modulates the antidiuretic actions of vasopressin in the euvolemic state.",
keywords = "diuresis, natriuresis, rat, renal medulla",
author = "C. Ledderhos and Mattson, {D. L.} and Skelton, {M. M.} and Cowley, {A. W.}",
year = "1995",
month = "1",
day = "1",
language = "English (US)",
volume = "268",
pages = "R796--R807",
journal = "American Journal of Physiology - Heart and Circulatory Physiology",
issn = "0363-6135",
publisher = "American Physiological Society",
number = "3 37-3",

}

TY - JOUR

T1 - In vivo diuretic actions of renal vasopressin V1 receptor stimulation in rats

AU - Ledderhos, C.

AU - Mattson, D. L.

AU - Skelton, M. M.

AU - Cowley, A. W.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - The specific vasopressin V1 receptor agonist (V1AG; [Phe2,Ile3,Orn8]vasopressin) was infused (2.0 ng · kg-1 · mi-1) into the renal medullary interstitial space to determine the effects of selective medullary V1 receptor stimulation on sodium and water excretion in normal rats. Responses were compared with those of arginine vasopressin (AVP) and vasopressin V2 receptor stimulation resulting from infusion of a V1 receptor antagonist with AVP. Medullary infusion of V1AG or AVP in euvolemic rats produced no changes in hemodynamics or glomerular filtration rate. V1AG increased urine flow > 60% in euvolemic rats, whereas no change was observed with AVP. This response could not be explained by a rise of arterial pressure or by volume retention. With V2 stimulation in euvolemic rats, urine flow was decreased. In water diuretic rats, V1AG produced no change, whereas AVP infusion decreased urine flow. The results provide in vivo evidence that tubular V1 vasopressin receptor activity results in increased urine flow and thereby modulates the antidiuretic actions of vasopressin in the euvolemic state.

AB - The specific vasopressin V1 receptor agonist (V1AG; [Phe2,Ile3,Orn8]vasopressin) was infused (2.0 ng · kg-1 · mi-1) into the renal medullary interstitial space to determine the effects of selective medullary V1 receptor stimulation on sodium and water excretion in normal rats. Responses were compared with those of arginine vasopressin (AVP) and vasopressin V2 receptor stimulation resulting from infusion of a V1 receptor antagonist with AVP. Medullary infusion of V1AG or AVP in euvolemic rats produced no changes in hemodynamics or glomerular filtration rate. V1AG increased urine flow > 60% in euvolemic rats, whereas no change was observed with AVP. This response could not be explained by a rise of arterial pressure or by volume retention. With V2 stimulation in euvolemic rats, urine flow was decreased. In water diuretic rats, V1AG produced no change, whereas AVP infusion decreased urine flow. The results provide in vivo evidence that tubular V1 vasopressin receptor activity results in increased urine flow and thereby modulates the antidiuretic actions of vasopressin in the euvolemic state.

KW - diuresis

KW - natriuresis

KW - rat

KW - renal medulla

UR - http://www.scopus.com/inward/record.url?scp=0028905478&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028905478&partnerID=8YFLogxK

M3 - Article

C2 - 7900923

AN - SCOPUS:0028905478

VL - 268

SP - R796-R807

JO - American Journal of Physiology - Heart and Circulatory Physiology

JF - American Journal of Physiology - Heart and Circulatory Physiology

SN - 0363-6135

IS - 3 37-3

ER -