In vivo effects of IL-4, IL-10, and amifostine on cytokine production in patients with acute myelogenous leukemia

M. Tao, Biaoru Li, J. Nayini, S. Sivaraman, S. Song, A. Larson, M. Toofanfard, H. Chen, P. Venugopal, H. D. Preisler

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Both IL-4 and IL-10 have been shown in vitro to inhibit leukemia cell secretion of IL-1β, GM-CSF, and TNFα, and increase leukemia cell release of IL-1ra. In this study, we have investigated the in vivo effects of IL-4, IL-10, and amifostine on cytokine production in patients with acute myelogenous leukemia (AML). Serum IL-1ra, IL-1β, TNFα, GM-CSF, and SCF levels were measured in AML patients who received IL-4, IL-10, or amifostine. No significant changes in the serum levels of IL-1ra, IL-1β, TNFα, GM-CSF, and SCF were found in AML patients who received amifostine. Both IL-4 and IL-10 were found to increase serum IL-1ra. This data is in accord with the in vitro studies. However, IL-4 increased serum GM-CSF levels and IL-10 increased serum IL-1β and TNFα levels. These in vivo effects of the two cytokines differ from their in vitro effects. Despite the similar effects of IL-4 and IL-10 on cytokine production by AML cells in vitro, different effects were observed in AML patients in vivo. IL-4 increased serum SCF levels, whereas IL-10 decreased serum SCF levels. IL-4 increased serum GM-CSF levels, whereas IL-10 had no effect on them. Although IL-10 increased serum IL-1β and TNFα levels, IL-4 had no effect on them. These findings indicate that the in vitro effects of IL-4 and IL-10 do not necessarily reflect their in vivo effects, and that the complex effects of the two cytokines on serum cytokine levels make it difficult to predict their therapeutic potential.

Original languageEnglish (US)
Pages (from-to)161-168
Number of pages8
JournalLeukemia and Lymphoma
Volume41
Issue number1-2
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

Fingerprint

Amifostine
Acute Myeloid Leukemia
Interleukin-4
Interleukin-10
Cytokines
Interleukin 1 Receptor Antagonist Protein
Granulocyte-Macrophage Colony-Stimulating Factor
Serum
Interleukin-1
Leukemia
In Vitro Techniques

Keywords

  • Acute myelogenous leukemia
  • Amifostine
  • IL-10
  • IL-4,
  • Serum cytokine levels

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

In vivo effects of IL-4, IL-10, and amifostine on cytokine production in patients with acute myelogenous leukemia. / Tao, M.; Li, Biaoru; Nayini, J.; Sivaraman, S.; Song, S.; Larson, A.; Toofanfard, M.; Chen, H.; Venugopal, P.; Preisler, H. D.

In: Leukemia and Lymphoma, Vol. 41, No. 1-2, 01.01.2001, p. 161-168.

Research output: Contribution to journalArticle

Tao, M, Li, B, Nayini, J, Sivaraman, S, Song, S, Larson, A, Toofanfard, M, Chen, H, Venugopal, P & Preisler, HD 2001, 'In vivo effects of IL-4, IL-10, and amifostine on cytokine production in patients with acute myelogenous leukemia', Leukemia and Lymphoma, vol. 41, no. 1-2, pp. 161-168. https://doi.org/10.3109/10428190109057966
Tao, M. ; Li, Biaoru ; Nayini, J. ; Sivaraman, S. ; Song, S. ; Larson, A. ; Toofanfard, M. ; Chen, H. ; Venugopal, P. ; Preisler, H. D. / In vivo effects of IL-4, IL-10, and amifostine on cytokine production in patients with acute myelogenous leukemia. In: Leukemia and Lymphoma. 2001 ; Vol. 41, No. 1-2. pp. 161-168.
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AU - Li, Biaoru

AU - Nayini, J.

AU - Sivaraman, S.

AU - Song, S.

AU - Larson, A.

AU - Toofanfard, M.

AU - Chen, H.

AU - Venugopal, P.

AU - Preisler, H. D.

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AB - Both IL-4 and IL-10 have been shown in vitro to inhibit leukemia cell secretion of IL-1β, GM-CSF, and TNFα, and increase leukemia cell release of IL-1ra. In this study, we have investigated the in vivo effects of IL-4, IL-10, and amifostine on cytokine production in patients with acute myelogenous leukemia (AML). Serum IL-1ra, IL-1β, TNFα, GM-CSF, and SCF levels were measured in AML patients who received IL-4, IL-10, or amifostine. No significant changes in the serum levels of IL-1ra, IL-1β, TNFα, GM-CSF, and SCF were found in AML patients who received amifostine. Both IL-4 and IL-10 were found to increase serum IL-1ra. This data is in accord with the in vitro studies. However, IL-4 increased serum GM-CSF levels and IL-10 increased serum IL-1β and TNFα levels. These in vivo effects of the two cytokines differ from their in vitro effects. Despite the similar effects of IL-4 and IL-10 on cytokine production by AML cells in vitro, different effects were observed in AML patients in vivo. IL-4 increased serum SCF levels, whereas IL-10 decreased serum SCF levels. IL-4 increased serum GM-CSF levels, whereas IL-10 had no effect on them. Although IL-10 increased serum IL-1β and TNFα levels, IL-4 had no effect on them. These findings indicate that the in vitro effects of IL-4 and IL-10 do not necessarily reflect their in vivo effects, and that the complex effects of the two cytokines on serum cytokine levels make it difficult to predict their therapeutic potential.

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