In vivo preservation of the hybrid layer by chlorhexidine

M. R.O. Carrilho, S. Geraldeli, F. Tay, M. F. De Goes, R. M. Carvalho, L. Tjäderhane, A. F. Reis, J. Hebling, A. Mazzoni, L. Breschi, D. Pashley

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423 Scopus citations


Host-derived proteases have been reported to degrade the collagen matrix of incompletely-resin-infiltrated dentin. This study tested the hypothesis that interfacial degradation of resin-dentin bonds may be prevented or delayed by the application of chlorhexidine (CHX), a matrix metalloproteinase inhibitor, to dentin after phosphoric acid-etching. Contralateral pairs of resin-bonded Class I restorations in non-carious third molars were kept under intra-oral function for 14 months. Preservation of resin-dentin bonds was assessed by microtensile bond strength tests and TEM examination. In vivo bond strength remained stable in the CHX-treated specimens, while bond strength decreased significantly in control teeth. Resin-infiltrated dentin in CHX-treated specimens exhibited normal structural integrity of the collagen network. Conversely, progressive disintegration of the fibrillar network was identified in control specimens. Auto-degradation of collagen matrices can occur in resin-infiltrated dentin, but may be prevented by the application of a synthetic protease inhibitor, such as chlorhexidine.

Original languageEnglish (US)
Pages (from-to)529-533
Number of pages5
JournalJournal of Dental Research
Issue number6
StatePublished - Jun 2007


  • Chlorhexidine
  • Degradation
  • Etch-and-rinse adhesive
  • Hybrid layer
  • In vivo
  • MMP

ASJC Scopus subject areas

  • Dentistry(all)


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