In vivo spin trapping of nitric oxide in mice

Andrei Komarov, David Mattson, Mark M. Jones, P. K. Singh, Ching San Lai

Research output: Contribution to journalArticle

Abstract

We report here an in vivo spin-trapping technique combined with electron paramagnetic resonance (EPR) spectroscopy to measure nitric oxide (·NO) production in the blood circulation of conscious mice. The method is based on the trapping of nitric oxide (·NO) by a metal-chelator complex consisting of N-methyl-D-glucamine dithiocarbamate (MGD) and reduced iron (Fe2+) to form a stable and water-soluble [(MGD)2-Fe2+-NO] complex, which gives rise to a characteristic three-line EPR spectrum of a mononitrosyl-Fe2+ complex (aN= 12.5 G and giso=2.04) at ambient temperatures. After simultaneous intravenous injection of sodium nitroprusside (an ·NO donor) and the [(MGD)2-Fe2+] complex into the lateral vein of the mouse tail, the appearance of the three-line spectrum in the blood circulation of the mouse tail was monitored continuously by using an S-band EPR spectrometer, operating at 3.5 GHz. This represents the first spin trapping of ·NO in living animals.

Original languageEnglish (US)
Pages (from-to)1191-1198
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume195
Issue number3
DOIs
StatePublished - Sep 30 1993
Externally publishedYes

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Spin Trapping
Electron Spin Resonance Spectroscopy
Nitric Oxide
Paramagnetic resonance
Blood Circulation
Hemodynamics
Tail
Nitric Oxide Donors
Coordination Complexes
Nitroprusside
Chelating Agents
Metal complexes
Intravenous Injections
Spectrometers
Veins
Spectrum Analysis
Animals
Iron
Spectroscopy
Temperature

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Komarov, A., Mattson, D., Jones, M. M., Singh, P. K., & Lai, C. S. (1993). In vivo spin trapping of nitric oxide in mice. Biochemical and Biophysical Research Communications, 195(3), 1191-1198. https://doi.org/10.1006/bbrc.1993.2170

In vivo spin trapping of nitric oxide in mice. / Komarov, Andrei; Mattson, David; Jones, Mark M.; Singh, P. K.; Lai, Ching San.

In: Biochemical and Biophysical Research Communications, Vol. 195, No. 3, 30.09.1993, p. 1191-1198.

Research output: Contribution to journalArticle

Komarov, A, Mattson, D, Jones, MM, Singh, PK & Lai, CS 1993, 'In vivo spin trapping of nitric oxide in mice', Biochemical and Biophysical Research Communications, vol. 195, no. 3, pp. 1191-1198. https://doi.org/10.1006/bbrc.1993.2170
Komarov, Andrei ; Mattson, David ; Jones, Mark M. ; Singh, P. K. ; Lai, Ching San. / In vivo spin trapping of nitric oxide in mice. In: Biochemical and Biophysical Research Communications. 1993 ; Vol. 195, No. 3. pp. 1191-1198.
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