In vivo temperature rise and acute inflammatory response in anesthetized human pulp tissue of premolars having Class V preparations after exposure to Polywave® LED light curing units

Dayane Jaqueline Gross, Andrés Dávila-Sánchez, Patrício Runnacles, Driellen Christine Zarpellon, Fagner Kiratcz, Eduardo Bauml Campagnoli, Luísa Fernanda Alegría-Acevedo, Ulisses Coelho, Frederick Allen Rueggeberg, Cesar Augusto Galvão Arrais

Research output: Contribution to journalArticle

Abstract

Objective: To evaluate the influence of light emitted from two Polywave®, LED light-curing units (LCU) on in vivo pulp temperature (PT) rise and signs of acute inflammatory response in pulps of human premolar having deep Class V preparations. Methods: Sixty intact, first premolars from 15 volunteers requiring extraction received infiltrative anesthesia. A sterile thermocouple probe was inserted within the pulp tissue through a minute occlusal pulp exposure in only 45 teeth (n = 9) to continuously monitor PT (°C). A deep buccal Class V preparation was created, and the surface was exposed to light from a commercial Polywave LCU (Bluephase 20i (20i), Ivoclar Vivadent) or from an experimental LCU (Exp) using the exposure modes (EM): 1s/Exp and 2s/Exp, 10s/20i, 20s/20i, and 60s/20i. Peak PT and PT rise values above baseline (ΔT) data were evaluated using a one-way ANOVA followed by Tukey's post-hoc test (α = 5%). Teeth used for histological and immunohistochemical analyses (n = 3) were extracted approximately 2 h after exposure to the LCU. Results: No significant difference in peak PT and ΔT values was noted between 2s/Exp and 20s/20i groups, which both exhibited higher values than 1s/Exp and 10s/20i groups (p < 0.001). Dilated and congested blood vessels were seen after exposure to 1s/Exp, 2s/Exp, or 60s/20i EMs. The expression of IL-1β and TNF-α tended to be more intense when higher irradiance was delivered. Significance: Although higher irradiance delivered over a short exposure caused lower PT rise than 5.5 °C, such EMs should be used with caution, as they have more potential to harm the pulp tissue.

Original languageEnglish (US)
Pages (from-to)1201-1213
Number of pages13
JournalDental Materials
Volume36
Issue number9
DOIs
StatePublished - Sep 2020

Keywords

  • Curing light
  • Histology
  • Humans
  • Immunohistochemistry
  • Pulp temperature
  • Random allocation

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Fingerprint Dive into the research topics of 'In vivo temperature rise and acute inflammatory response in anesthetized human pulp tissue of premolars having Class V preparations after exposure to Polywave® LED light curing units'. Together they form a unique fingerprint.

  • Cite this

    Gross, D. J., Dávila-Sánchez, A., Runnacles, P., Zarpellon, D. C., Kiratcz, F., Campagnoli, E. B., Alegría-Acevedo, L. F., Coelho, U., Rueggeberg, F. A., & Arrais, C. A. G. (2020). In vivo temperature rise and acute inflammatory response in anesthetized human pulp tissue of premolars having Class V preparations after exposure to Polywave® LED light curing units. Dental Materials, 36(9), 1201-1213. https://doi.org/10.1016/j.dental.2020.05.015