Inactivating Mutations in GT198 in Familial and Early-Onset Breast and Ovarian Cancers

Min Peng, Janine L. Bakker, Richard A. DiCioccio, Johan J.P. Gille, Hua Zhao, Kunle Odunsi, Lara Sucheston, Lahcen Jaafar, Nahid F Mivechi, Quinten Waisfisz, Lan Ko

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The human GT198 gene (gene symbol PSMC3IP) is located at chromosome 17q21, 470 kb proximal to BRCA1, a locus previously linked to breast and ovarian cancer predisposition. Its protein product (also known as TBPIP and Hop2) has been shown to regulate steroid hormone receptor-mediated gene activation and to stimulate homologous recombination in DNA repair. Here, we screened germline mutations in GT198 in familial and early-onset breast and ovarian cancer patients. We have identified 8 germline variants in a total of 212 index patients including reoccurring nonsense mutation c.310C>T (p.Q104X) and 5′ UTR mutation c.-37A>T, each found in 2 unrelated families. Most identified index patients from cancer families had early onsets with a median age of 35 years. c.310C>T was absent in a total of 564 control individuals analyzed. GT198 gene amplification with an imbalanced mutant copy gain was identified in the blood DNA of one of the patients carrying c.310C>T. When tested, this truncating mutation abolished DNA damage-induced Rad51 foci formation. In addition, we have identified 15 somatic mutations in 2 tumors from 1 patient carrying germline mutation c.-37A>T. The presence of a somatic mutation on the wild-type allele showed that GT198 was biallelically mutated in the tumor. The somatic mutations identified near a splicing junction site caused defective alternative splicing and truncated the open reading frame. Therefore, distinct mutations may cause a similar consequence by truncating the full-length protein and inducing a loss of the wild type. Our study provides the first evidence of the presence of inactivating mutations in GT198 in familial and early-onset breast and ovarian cancer patients. Mutations in GT198, a gene regulating DNA repair, potentially contribute to an increased risk in familial breast and ovarian cancers.

Original languageEnglish (US)
Pages (from-to)15-25
Number of pages11
JournalGenes and Cancer
Volume4
Issue number1-2
DOIs
StatePublished - Mar 1 2013

Keywords

  • GT198
  • breast and ovarian cancer
  • gene amplification
  • mutation

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

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    Peng, M., Bakker, J. L., DiCioccio, R. A., Gille, J. J. P., Zhao, H., Odunsi, K., Sucheston, L., Jaafar, L., Mivechi, N. F., Waisfisz, Q., & Ko, L. (2013). Inactivating Mutations in GT198 in Familial and Early-Onset Breast and Ovarian Cancers. Genes and Cancer, 4(1-2), 15-25. https://doi.org/10.1177/1947601913486344