Inaktivatsiia i stabilizatsiia fibrinoliticheskoi activnosti urokinazy in vitro.

Translated title of the contribution: Inactivation and stabilization of urokinase fibrinolytic activity in vitro

R. B. Aisina, L. I. Mukhametova, I. I. Sazonova, S. D. Varfolomeev

Research output: Contribution to journalArticle

Abstract

When incubated at 30 degrees C and pH 7.4, urokinase lost fibrinolytic activity (i.e., the plasminogen-activating activity measured by the time of fibrin clot lysis) but completely retained amidase activity. The enzyme inactivation rate depended on the urokinase concentration and, at concentrations of more than 1.5 microM, was described by a second order equation, which indicated that the enzyme underwent autolytic degradation (kaut = 3.8 x 10(-3) M-1 min-1). During incubation, urokinase (54 kDa) was converted into its low-molecular-mass form (33 kDa) and products of the A-chain degradation. The amidase activity did not correlate with the fibrinolytic activity in the cases when the enzyme molecule underwent local unfolding or partial degradation. The optimum mixture of agents for stabilizing the fibrinolytic activity of urokinase was found.

Original languageRussian
Pages (from-to)544-548
Number of pages5
JournalBioorganicheskaia khimiia
Volume24
Issue number7
StatePublished - Jul 1 1998

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Urokinase-Type Plasminogen Activator
amidase
Enzymes
Fibrin Clot Lysis Time
Fibrinolytic Agents
Excipients
Plasminogen
In Vitro Techniques

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Aisina, R. B., Mukhametova, L. I., Sazonova, I. I., & Varfolomeev, S. D. (1998). Inaktivatsiia i stabilizatsiia fibrinoliticheskoi activnosti urokinazy in vitro. Bioorganicheskaia khimiia, 24(7), 544-548.

Inaktivatsiia i stabilizatsiia fibrinoliticheskoi activnosti urokinazy in vitro. / Aisina, R. B.; Mukhametova, L. I.; Sazonova, I. I.; Varfolomeev, S. D.

In: Bioorganicheskaia khimiia, Vol. 24, No. 7, 01.07.1998, p. 544-548.

Research output: Contribution to journalArticle

Aisina, RB, Mukhametova, LI, Sazonova, II & Varfolomeev, SD 1998, 'Inaktivatsiia i stabilizatsiia fibrinoliticheskoi activnosti urokinazy in vitro.', Bioorganicheskaia khimiia, vol. 24, no. 7, pp. 544-548.
Aisina RB, Mukhametova LI, Sazonova II, Varfolomeev SD. Inaktivatsiia i stabilizatsiia fibrinoliticheskoi activnosti urokinazy in vitro. Bioorganicheskaia khimiia. 1998 Jul 1;24(7):544-548.
Aisina, R. B. ; Mukhametova, L. I. ; Sazonova, I. I. ; Varfolomeev, S. D. / Inaktivatsiia i stabilizatsiia fibrinoliticheskoi activnosti urokinazy in vitro. In: Bioorganicheskaia khimiia. 1998 ; Vol. 24, No. 7. pp. 544-548.
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