Inactivation of viruses infecting ectothermic animals by amphibian and piscine antimicrobial peptides

V. G. Chinchar, Locke Johnson Bryan, U. Silphadaung, E. Noga, D. Wade, L. Rollins-Smith

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

The ability of five purified amphibian antimicrobial peptides (dermaseptin-1, temporin A, magainin I, and II, PGLa), crude peptide fractions isolated from the skin of Rana pipiens and R. catesbeiana, and four antimicrobial peptides (AMPs) from hybrid striped bass (piscidin-1N, -1H, -2, and -3) were examined for their ability to reduce the infectivity of channel catfish virus (CCV) and frog virus 3 (FV3). All compounds, with the exception of magainin I, markedly reduced the infectivity of CCV. In contrast to CCV, FV3 was 2- to 4-fold less sensitive to these agents. Similar to an earlier study employing two other amphibian peptides, the agents used here acted rapidly and over a wide, physiologically relevant, temperature range to reduce virus infectivity. These results extend our previous findings and strongly suggest that various amphibian and piscine AMPs may play important roles in protecting fish and amphibians from pathogenic viruses.

Original languageEnglish (US)
Pages (from-to)268-275
Number of pages8
JournalVirology
Volume323
Issue number2
DOIs
StatePublished - Jun 1 2004

Fingerprint

Virus Inactivation
Ictalurivirus
Amphibians
Ranavirus
Magainins
Peptides
Viruses
Rana pipiens
Bass
Fishes
Skin
Temperature

Keywords

  • Amphibian decline
  • Antimicrobial peptides
  • Channel catfish virus
  • Dermaseptin
  • Ectothermic organisms
  • Frog virus 3
  • Herpesvirus
  • Iridovirus
  • Magainin
  • Piscidins
  • Ranatuerin
  • Temporin

ASJC Scopus subject areas

  • Virology

Cite this

Inactivation of viruses infecting ectothermic animals by amphibian and piscine antimicrobial peptides. / Chinchar, V. G.; Bryan, Locke Johnson; Silphadaung, U.; Noga, E.; Wade, D.; Rollins-Smith, L.

In: Virology, Vol. 323, No. 2, 01.06.2004, p. 268-275.

Research output: Contribution to journalArticle

Chinchar, V. G. ; Bryan, Locke Johnson ; Silphadaung, U. ; Noga, E. ; Wade, D. ; Rollins-Smith, L. / Inactivation of viruses infecting ectothermic animals by amphibian and piscine antimicrobial peptides. In: Virology. 2004 ; Vol. 323, No. 2. pp. 268-275.
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