INCANDESCENT LAMPS CAN PRODUCE PYRIMIDINE DIMERS IN DNA

Giovanni Ciarrocchi, Betsy M. Sutherland, John C. Sutherland

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

DNA molecules that have been exposed to light from a 150 W incandescent spot lamp are nicked by the Micrococcus luteus endonuclease specific for cyclobutyl‐type pyrimidine dimers. The production of these enzyme‐sensitive sites increases with increasing spot lamp exposure. These sites have been confirmed to be pyrimidine dimers by their property of being photoreversed by an E. coli photoreactivating enzyme. The emission spectrum of the lamp shows detectable output at wavelengths less than 320 nm. These results indicate that the sensitivity of the techniques utilized in this work can be used to detect low levels of contaminating UV radiation.

Original languageEnglish (US)
Pages (from-to)703-705
Number of pages3
JournalPhotochemistry and Photobiology
Volume41
Issue number6
DOIs
StatePublished - Jan 1 1985
Externally publishedYes

Fingerprint

Incandescent lamps
Pyrimidine Dimers
pyrimidines
Electric lamps
luminaires
deoxyribonucleic acid
Deoxyribodipyrimidine Photo-Lyase
dimers
Micrococcus luteus
Endonucleases
DNA
Radiation
Escherichia coli
Light
Ultraviolet radiation
enzymes
emission spectra
Wavelength
Molecules
output

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry
  • Physical and Theoretical Chemistry

Cite this

INCANDESCENT LAMPS CAN PRODUCE PYRIMIDINE DIMERS IN DNA. / Ciarrocchi, Giovanni; Sutherland, Betsy M.; Sutherland, John C.

In: Photochemistry and Photobiology, Vol. 41, No. 6, 01.01.1985, p. 703-705.

Research output: Contribution to journalArticle

Ciarrocchi, Giovanni ; Sutherland, Betsy M. ; Sutherland, John C. / INCANDESCENT LAMPS CAN PRODUCE PYRIMIDINE DIMERS IN DNA. In: Photochemistry and Photobiology. 1985 ; Vol. 41, No. 6. pp. 703-705.
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