Increase in free cholesterol content of the adrenal cortex after stress: Radioautographic and biochemical study

Mohamed M.H. Sharawy, Thomas Dirksen, Joe Chaffin

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The purpose of this investigation was to quantify free cholesterol biochemically and in radioautographs of 3H‐digitonin cholesterol complex in fasciculata cells of control and stressed rat adrenal cortex. Stress was induced by ether, laparotomy, and adrenal and intestinal handling. Control rats were anesthetized with nembutal. All animals were killed ten minutes from the beginning of anesthesia. The adrenals were excised and either fixed in glutaraldehyde containing 3H‐digitonin or homogenized for biochemical determination of free cholesterol. The plasma corticosterone level of each animal was measured. The fixed adrenals were processed, using different methods of dehydration and embedment, for light and electron microscopic radioautography. The mean number of silver grains (X̄) per unit area of zona fasciculata was counted from light microscopic radioautographs. Crystals of cholesterol‐digitonide complex were more numerous in stressed fasciculata cells, particularly over SER. Silver grains were localized over or close to the crystals. The X̄ for stressed rats was significantly higher than control values, indicating more free cholesterol in fasciculata cells of stressed rats. The results were not affected by either the method of dehydration or the type of embedding medium used. The morphologic results were substantiated by biochemical findings of increase in free cholesterol in adrenals of stressed rats. Plasma corticosterone was significantly high in stressed rats. The increase in free cholesterol in stimulated fasciculata cells is consistent with a previously reported increase in cholesterol esterase activity after ACTH stimulation.

Original languageEnglish (US)
Pages (from-to)567-575
Number of pages9
JournalAmerican Journal of Anatomy
Volume156
Issue number4
DOIs
StatePublished - Jan 1 1979

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Adrenal Cortex
Cholesterol
Corticosterone
Dehydration
Silver
Zona Fasciculata
Sterol Esterase
Light
Glutaral
Pentobarbital
Autoradiography
Ether
Adrenocorticotropic Hormone
Laparotomy
Anesthesia
Electrons

ASJC Scopus subject areas

  • Anatomy

Cite this

Increase in free cholesterol content of the adrenal cortex after stress : Radioautographic and biochemical study. / Sharawy, Mohamed M.H.; Dirksen, Thomas; Chaffin, Joe.

In: American Journal of Anatomy, Vol. 156, No. 4, 01.01.1979, p. 567-575.

Research output: Contribution to journalArticle

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