Increase of sodium delivery stimulates the mitochondrial respiratory chain H 2O 2 production in rat renal medullary thick ascending limb

Yusuke Ohsaki, Paul M O'Connor, Takefumi Mori, Robert P. Ryan, Bryan C. Dickinson, Christopher J. Chang, Yi Lu, Sadayoshi Ito, Allen W. Cowley

Research output: Contribution to journalArticle

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Abstract

The mitochondria-rich epithelial cells of the renal medullary thick ascending limb (mTAL) reabsorb nearly 25% of filtered sodium (Na +) and are a major source of cellular reactive oxygen species. Although we have shown that delivery of Na + to the mTAL of rats increases superoxide (O2 -) production in mTAL, little is known about H 2O 2 production, given the lack of robust and selective fluorescent indicators for determining changes within the whole cell, specifically in the mitochondria. The present study determined the effect of increased tubular flow and Na + delivery to mTAL on the production of mitochondrial H 2O 2 in mTAL. H 2O 2 responses were determined in isolated, perfused mTAL of Sprague-Dawley rats using a novel mitochondrial selective fluorescent H 2O 2 indicator, mitochondria peroxy yellow 1, and a novel, highly sensitive and stable cytosolic-localized H 2O 2 indicator, peroxyfluor- 6 acetoxymethyl ester. The results showed that mitochondrial H 2O 2 and cellular fluorescent signals increased progressively over a period of 30 min following increased tubular perfusion (5-20 nl/min), reaching levels of statistical significance at ∼10-12 min. Responses were inhibited with rotenone or antimycin A (inhibitors of the electron-transport chain), polyethylene glycol-catalase and by reducing Na + transport with furosemide or ouabain. Inhibition of membrane NADPH-oxidase with apocynin had no effect on mitochondrial H 2O 2 production. Cytoplasmic H 2O 2 (peroxyfluor-6 acetoxymethyl ester) increased in parallel with mitochondrial H 2O 2 (mitochondria peroxy yellow 1) and was partially attenuated (∼65%) by rotenone and completely inhibited by apocynin. The present data provide clear evidence that H 2O 2 is produced in the mitochondria in response to increased flow and delivery of Na_ to the mTAL, and that whole cell H 2O 2 levels are triggered by the mitochondrial reactive oxygen species production. The mitochondrial production of H 2O 2 may represent an important target for development of more effective antioxidant therapies.

Original languageEnglish (US)
Pages (from-to)95-102
Number of pages8
JournalAmerican Journal of Physiology - Renal Physiology
Volume302
Issue number1
DOIs
StatePublished - Jan 1 2012
Externally publishedYes

Fingerprint

Electron Transport
Extremities
Sodium
Kidney
Mitochondria
Rotenone
Reactive Oxygen Species
Esters
Antimycin A
NADPH Oxidase
Furosemide
Ouabain
Superoxides
Sprague Dawley Rats
Perfusion
Antioxidants
Epithelial Cells
Membranes

Keywords

  • Flow
  • Kidney
  • Mitochondria
  • Reactive oxygen species

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

Increase of sodium delivery stimulates the mitochondrial respiratory chain H 2O 2 production in rat renal medullary thick ascending limb. / Ohsaki, Yusuke; O'Connor, Paul M; Mori, Takefumi; Ryan, Robert P.; Dickinson, Bryan C.; Chang, Christopher J.; Lu, Yi; Ito, Sadayoshi; Cowley, Allen W.

In: American Journal of Physiology - Renal Physiology, Vol. 302, No. 1, 01.01.2012, p. 95-102.

Research output: Contribution to journalArticle

Ohsaki, Yusuke ; O'Connor, Paul M ; Mori, Takefumi ; Ryan, Robert P. ; Dickinson, Bryan C. ; Chang, Christopher J. ; Lu, Yi ; Ito, Sadayoshi ; Cowley, Allen W. / Increase of sodium delivery stimulates the mitochondrial respiratory chain H 2O 2 production in rat renal medullary thick ascending limb. In: American Journal of Physiology - Renal Physiology. 2012 ; Vol. 302, No. 1. pp. 95-102.
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