Increased 8-OHdG levels in the urine, serum, and substantia nigra of hemiparkinsonian rats

Takao Yasuhara, Koichi Hara, Kapil Dev Sethi, John Christopher Morgan, Cesario V. Borlongan

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

8-Hydroxy-2′-deoxyguanosine (8-OHdG), the predominant marker of oxidative DNA damage, may be a good biomarker for monitoring the progression of Parkinson's disease (PD). Unfortunately, there are no basic laboratory data examining 8-OHdG levels in animal models of PD. In this study, we demonstrate that rats lesioned with 6-hydroxydopamine (6-OHDA) in the medial forebrain bundle display significantly elevated 8-OHdG levels in urine, serum, and substantia nigra, but not cerebrospinal fluid and striatum, compared to sham controls. These increments in 8-OHdG levels were detected at 2 days, but not at 7 days after the lesion suggesting that oxidative stress is restricted to the acute phase of 6-OHDA neurotoxicity. The present results support 8-OHdG as a biomarker that may aid both in the diagnosis and in the documentation of progression in PD.

Original languageEnglish (US)
Pages (from-to)49-52
Number of pages4
JournalBrain Research
Volume1133
Issue number1
DOIs
StatePublished - Jan 16 2007

Fingerprint

Oxidopamine
Substantia Nigra
Parkinson Disease
Urine
Biomarkers
Serum
Medial Forebrain Bundle
Documentation
DNA Damage
Cerebrospinal Fluid
Oxidative Stress
Animal Models

Keywords

  • 8-OHdG
  • Biomarker
  • Neurodegeneration
  • Oxidative stress
  • Parkinson's disease

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

Increased 8-OHdG levels in the urine, serum, and substantia nigra of hemiparkinsonian rats. / Yasuhara, Takao; Hara, Koichi; Sethi, Kapil Dev; Morgan, John Christopher; Borlongan, Cesario V.

In: Brain Research, Vol. 1133, No. 1, 16.01.2007, p. 49-52.

Research output: Contribution to journalArticle

Yasuhara, Takao ; Hara, Koichi ; Sethi, Kapil Dev ; Morgan, John Christopher ; Borlongan, Cesario V. / Increased 8-OHdG levels in the urine, serum, and substantia nigra of hemiparkinsonian rats. In: Brain Research. 2007 ; Vol. 1133, No. 1. pp. 49-52.
@article{c2ca041331fc4ae49e525d9b5ade0ed8,
title = "Increased 8-OHdG levels in the urine, serum, and substantia nigra of hemiparkinsonian rats",
abstract = "8-Hydroxy-2′-deoxyguanosine (8-OHdG), the predominant marker of oxidative DNA damage, may be a good biomarker for monitoring the progression of Parkinson's disease (PD). Unfortunately, there are no basic laboratory data examining 8-OHdG levels in animal models of PD. In this study, we demonstrate that rats lesioned with 6-hydroxydopamine (6-OHDA) in the medial forebrain bundle display significantly elevated 8-OHdG levels in urine, serum, and substantia nigra, but not cerebrospinal fluid and striatum, compared to sham controls. These increments in 8-OHdG levels were detected at 2 days, but not at 7 days after the lesion suggesting that oxidative stress is restricted to the acute phase of 6-OHDA neurotoxicity. The present results support 8-OHdG as a biomarker that may aid both in the diagnosis and in the documentation of progression in PD.",
keywords = "8-OHdG, Biomarker, Neurodegeneration, Oxidative stress, Parkinson's disease",
author = "Takao Yasuhara and Koichi Hara and Sethi, {Kapil Dev} and Morgan, {John Christopher} and Borlongan, {Cesario V.}",
year = "2007",
month = "1",
day = "16",
doi = "10.1016/j.brainres.2006.11.072",
language = "English (US)",
volume = "1133",
pages = "49--52",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Increased 8-OHdG levels in the urine, serum, and substantia nigra of hemiparkinsonian rats

AU - Yasuhara, Takao

AU - Hara, Koichi

AU - Sethi, Kapil Dev

AU - Morgan, John Christopher

AU - Borlongan, Cesario V.

PY - 2007/1/16

Y1 - 2007/1/16

N2 - 8-Hydroxy-2′-deoxyguanosine (8-OHdG), the predominant marker of oxidative DNA damage, may be a good biomarker for monitoring the progression of Parkinson's disease (PD). Unfortunately, there are no basic laboratory data examining 8-OHdG levels in animal models of PD. In this study, we demonstrate that rats lesioned with 6-hydroxydopamine (6-OHDA) in the medial forebrain bundle display significantly elevated 8-OHdG levels in urine, serum, and substantia nigra, but not cerebrospinal fluid and striatum, compared to sham controls. These increments in 8-OHdG levels were detected at 2 days, but not at 7 days after the lesion suggesting that oxidative stress is restricted to the acute phase of 6-OHDA neurotoxicity. The present results support 8-OHdG as a biomarker that may aid both in the diagnosis and in the documentation of progression in PD.

AB - 8-Hydroxy-2′-deoxyguanosine (8-OHdG), the predominant marker of oxidative DNA damage, may be a good biomarker for monitoring the progression of Parkinson's disease (PD). Unfortunately, there are no basic laboratory data examining 8-OHdG levels in animal models of PD. In this study, we demonstrate that rats lesioned with 6-hydroxydopamine (6-OHDA) in the medial forebrain bundle display significantly elevated 8-OHdG levels in urine, serum, and substantia nigra, but not cerebrospinal fluid and striatum, compared to sham controls. These increments in 8-OHdG levels were detected at 2 days, but not at 7 days after the lesion suggesting that oxidative stress is restricted to the acute phase of 6-OHDA neurotoxicity. The present results support 8-OHdG as a biomarker that may aid both in the diagnosis and in the documentation of progression in PD.

KW - 8-OHdG

KW - Biomarker

KW - Neurodegeneration

KW - Oxidative stress

KW - Parkinson's disease

UR - http://www.scopus.com/inward/record.url?scp=33846315383&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846315383&partnerID=8YFLogxK

U2 - 10.1016/j.brainres.2006.11.072

DO - 10.1016/j.brainres.2006.11.072

M3 - Article

VL - 1133

SP - 49

EP - 52

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -