Increased ENaC activity during kidney preservation in Wisconsin solution

Sherif Khedr, Oleg Palygin, Tengis S. Pavlov, Gregory Blass, Vladislav Levchenko, Ammar Alsheikh, Michael W Brands, Ashraf El-Meanawy, Alexander Staruschenko

Research output: Contribution to journalArticle

Abstract

Background: The invention of an effective kidney preservation solution capable of prolonging harvested kidney viability is the core of kidney transplantation procedure. Researchers have been working on upgrading the preservation solution quality aiming at prolonging storage time while maintaining utmost organ viability and functionality. For many years, the University of Wisconsin (UW) solution has been considered the gold standard solution for kidney preservation. However, the lifespan of kidney preservation in the UW solution is still limited. Its impact on the epithelial Na + channel (ENaC) activity and its mediated processes is unknown and the primary goal of this study. Methods: Kidneys harvested from 8 weeks old Sprague Dawley rats were divided into 4 groups depending upon the period of preservation in UW solution. Additional analysis was performed using dogs' kidneys. ENaC activity was measured using patch clamp technique; protein expression and mRNA transcription were tested through Western blot and RT-qPCR, respectively. A colorimetric LDH level estimation was performed at different time points during UW solution preservation. Results: Kidney preservation in Wisconsin solution caused reduction of the kidney size and weight and elevation of LDH level. ENaC activity increased in both rat and dog kidneys preserved in the UW solution as assessed by patch clamp analysis. On the contrary, ENaC channel mRNA levels remained unchanged. Conclusions: ENaC activity is significantly elevated in the kidneys during preservation in UW solution, which might affect the immediate post-implantation allograft function and trajectory post-transplant.

Original languageEnglish (US)
Article number145
JournalBMC Nephrology
Volume20
Issue number1
DOIs
StatePublished - Apr 29 2019

Fingerprint

Epithelial Sodium Channels
Kidney
Dogs
Tissue Survival
Messenger RNA
Patch-Clamp Techniques
Kidney Transplantation
Allografts
Sprague Dawley Rats
Western Blotting
Research Personnel

Keywords

  • ENaC
  • Kidney transplantation
  • Shrinkage
  • Wisconsin solution

ASJC Scopus subject areas

  • Nephrology

Cite this

Khedr, S., Palygin, O., Pavlov, T. S., Blass, G., Levchenko, V., Alsheikh, A., ... Staruschenko, A. (2019). Increased ENaC activity during kidney preservation in Wisconsin solution. BMC Nephrology, 20(1), [145]. https://doi.org/10.1186/s12882-019-1329-7

Increased ENaC activity during kidney preservation in Wisconsin solution. / Khedr, Sherif; Palygin, Oleg; Pavlov, Tengis S.; Blass, Gregory; Levchenko, Vladislav; Alsheikh, Ammar; Brands, Michael W; El-Meanawy, Ashraf; Staruschenko, Alexander.

In: BMC Nephrology, Vol. 20, No. 1, 145, 29.04.2019.

Research output: Contribution to journalArticle

Khedr, S, Palygin, O, Pavlov, TS, Blass, G, Levchenko, V, Alsheikh, A, Brands, MW, El-Meanawy, A & Staruschenko, A 2019, 'Increased ENaC activity during kidney preservation in Wisconsin solution', BMC Nephrology, vol. 20, no. 1, 145. https://doi.org/10.1186/s12882-019-1329-7
Khedr S, Palygin O, Pavlov TS, Blass G, Levchenko V, Alsheikh A et al. Increased ENaC activity during kidney preservation in Wisconsin solution. BMC Nephrology. 2019 Apr 29;20(1). 145. https://doi.org/10.1186/s12882-019-1329-7
Khedr, Sherif ; Palygin, Oleg ; Pavlov, Tengis S. ; Blass, Gregory ; Levchenko, Vladislav ; Alsheikh, Ammar ; Brands, Michael W ; El-Meanawy, Ashraf ; Staruschenko, Alexander. / Increased ENaC activity during kidney preservation in Wisconsin solution. In: BMC Nephrology. 2019 ; Vol. 20, No. 1.
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