Increased lymphoid tissue apoptosis in baboons with bacteremic shock.

Philip A. Efron, Kevin Tinsley, Douglas J. Minnich, Victor Hugo Monterroso, J. Wagner, Pierre Lainée, Katrien Lorré, Paul E. Swanson, Richard Hotchkiss, Lyle L. Moldawer

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The molecular mechanisms of immune cell apoptosis during sepsis remain unclear. Two young adult baboons (Papio sp.) received a lethal dose of live Escherichia coli and were sacrificed at either 16 (for animal welfare concerns) or 24 h post-septic shock. An additional baboon, which received no bacteria, served as a control. Necropsy was performed immediately with subsequent immunohistochemical staining of lymphoid tissue. Immunohistologic analysis of tissues from the septic baboons revealed marked systemic lymphocyte apoptosis occurring in all lymphoid tissues examined. Focally, pyknotic and karyorrhectic lymphocytes demonstrated activation of a mitochondrial-dependent cell death pathway (active caspase 9 and apoptosis-inducing factor). Other regions demonstrated apoptotic lymphocytes with activation of a death receptor-dependent cell pathway (Fas ligand). Thus, we have demonstrated for the first time in primates that overwhelming gram-negative bacteremia produces an early and profound lymphocyte death that occurs through multiple cell death pathways. Bacteremic shock in the baboon may be an appropriate model for studying experimental therapies aimed at blocking lymphocyte apoptosis because their response appears comparable to humans dying from sepsis.

Original languageEnglish (US)
Pages (from-to)566-571
Number of pages6
JournalShock (Augusta, Ga.)
Volume21
Issue number6
DOIs
StatePublished - Jan 1 2004

Fingerprint

Papio
Lymphoid Tissue
Shock
Apoptosis
Lymphocytes
Lymphocyte Activation
Sepsis
Cell Death
Apoptosis Inducing Factor
Death Domain Receptors
Animal Welfare
Investigational Therapies
Fas Ligand Protein
Caspase 9
Septic Shock
Bacteremia
Primates
Young Adult
Staining and Labeling
Escherichia coli

ASJC Scopus subject areas

  • Emergency Medicine
  • Critical Care and Intensive Care Medicine

Cite this

Efron, P. A., Tinsley, K., Minnich, D. J., Monterroso, V. H., Wagner, J., Lainée, P., ... Moldawer, L. L. (2004). Increased lymphoid tissue apoptosis in baboons with bacteremic shock. Shock (Augusta, Ga.), 21(6), 566-571. https://doi.org/10.1097/01.shk.0000126648.58732.8c

Increased lymphoid tissue apoptosis in baboons with bacteremic shock. / Efron, Philip A.; Tinsley, Kevin; Minnich, Douglas J.; Monterroso, Victor Hugo; Wagner, J.; Lainée, Pierre; Lorré, Katrien; Swanson, Paul E.; Hotchkiss, Richard; Moldawer, Lyle L.

In: Shock (Augusta, Ga.), Vol. 21, No. 6, 01.01.2004, p. 566-571.

Research output: Contribution to journalArticle

Efron, PA, Tinsley, K, Minnich, DJ, Monterroso, VH, Wagner, J, Lainée, P, Lorré, K, Swanson, PE, Hotchkiss, R & Moldawer, LL 2004, 'Increased lymphoid tissue apoptosis in baboons with bacteremic shock.', Shock (Augusta, Ga.), vol. 21, no. 6, pp. 566-571. https://doi.org/10.1097/01.shk.0000126648.58732.8c
Efron PA, Tinsley K, Minnich DJ, Monterroso VH, Wagner J, Lainée P et al. Increased lymphoid tissue apoptosis in baboons with bacteremic shock. Shock (Augusta, Ga.). 2004 Jan 1;21(6):566-571. https://doi.org/10.1097/01.shk.0000126648.58732.8c
Efron, Philip A. ; Tinsley, Kevin ; Minnich, Douglas J. ; Monterroso, Victor Hugo ; Wagner, J. ; Lainée, Pierre ; Lorré, Katrien ; Swanson, Paul E. ; Hotchkiss, Richard ; Moldawer, Lyle L. / Increased lymphoid tissue apoptosis in baboons with bacteremic shock. In: Shock (Augusta, Ga.). 2004 ; Vol. 21, No. 6. pp. 566-571.
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