Increased NADPH oxidase-derived superoxide is involved in the neuronal cell death induced by hypoxiaischemia in neonatal hippocampal slice cultures

Qing Lu, Mark S. Wainwright, Valerie A. Harris, Saurabh Aggarwal, Yali Hou, Thomas Rau, David J. Poulsen, Stephen Matthew Black

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Neonatal brain hypoxiaischemia (HI) results in neuronal cell death. Previous studies indicate that reactive oxygen species, such as superoxide, play a key role in this process. However, the cellular sources have not been established. In this study we examine the role of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase complex in neonatal HI brain injury and elucidate its mechanism of activation. Rat hippocampal slices were exposed to oxygen glucose deprivation (OGD) to mimic the conditions seen in HI. Initial studies confirmed an important role for NADPH oxidase-derived superoxide in the oxidative stress associated with OGD. Further, the OGD-mediated increase in apoptotic cell death was inhibited by the NADPH oxidase inhibitor apocynin. The activation of NADPH oxidase was found to be dependent on the p38 mitogen-activated protein kinase-mediated phosphorylation and activation of the p47phox subunit. Using an adeno-associated virus antisense construct to selectively decrease p47phox expression in neurons showed that this led to inhibition of both the increase in superoxide and the neuronal cell death associated with OGD. We also found that NADPH oxidase inhibition in a neonatal rat model of HI or scavenging hydrogen peroxide reduced brain injury. Thus, we conclude that activation of the NADPH oxidase complex contributes to the oxidative stress during HI and that therapies targeted against this complex could provide neuroprotection against the brain injury associated with neonatal HI.

Original languageEnglish (US)
Pages (from-to)1139-1151
Number of pages13
JournalFree Radical Biology and Medicine
Volume53
Issue number5
DOIs
StatePublished - Sep 1 2012

Keywords

  • Apoptosis
  • Free radicals
  • Hydrogen peroxide
  • Hypoxiaischemia
  • NADPH oxidase
  • Neonatal brain
  • Neuronal cell death
  • Superoxide
  • p38MAP kinase
  • p47

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

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