Increased nitric oxide synthase activity and Hsp90 association in skeletal muscle following chronic exercise

M. Brennan Harris, Brett M. Mitchell, Sarika G. Sood, R Clinton Webb, Richard C Venema

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Exercise training results in dynamic changes in skeletal muscle blood flow and metabolism. Nitric oxide (NO) influences blood flow, oxidative stress, and glucose metabolism. Hsp90 interacts directly with nitric oxide synthases (NOS), increasing NOS activity and altering the balance of superoxide versus NO production. In addition, Hsp90 expression increases in various tissues following exercise. Therefore, we tested the hypothesis that exercise training increases Hsp90 expression as well as Hsp90/NOS association and NOS activity in skeletal muscle. Male, Sprague-Dawley rats were assigned to either a sedentary or exercise trained group (n = 10/group). Exercise training consisted of running on a motorized treadmill for 10 weeks at 30 m/min, 5% grade for 1 h. Western blotting revealed that exercise training resulted in a 1.9 ± 0.1-fold increase in Hsp90 expression in the soleus muscle but no increase in neuronal nitric oxide synthase (nNOS), inducible nitric oxide synthase, or endothelial nitric oxide synthase (eNOS). Exercise training also resulted in a 3.4 ± 1.0-fold increase in Hsp90 association with nNOS, a 2.3 ± 0.4-fold increase association with eNOS measured by immunoprecipitation as well as a 1.5 ± 0.3-fold increase in eNOS phosphorylation at Ser-1179. Total NOS activity measured by the rate of conversion of L-[14 C]arginine to L-[14C]citrulline was increased by 1.42 ± 0.9 fold in soleus muscle following exercise training compared to controls. In summary, a 10-week treadmill training program in rats results in a significant increase in total NOS activity in the soleus which may be due, in part, to increased NOS interaction with Hsp90 and phosphorylation. This interaction may play a role in altering muscle blood flow and skeletal muscle redox status.

Original languageEnglish (US)
Pages (from-to)795-802
Number of pages8
JournalEuropean Journal of Applied Physiology
Volume104
Issue number5
DOIs
StatePublished - Sep 12 2008

Fingerprint

Nitric Oxide Synthase
Skeletal Muscle
Exercise
Nitric Oxide Synthase Type III
Nitric Oxide Synthase Type I
Nitric Oxide
Phosphorylation
Citrulline
Nitric Oxide Synthase Type II
Immunoprecipitation
Superoxides
Running
Oxidation-Reduction
Sprague Dawley Rats
Arginine
Oxidative Stress
Western Blotting
Education
Glucose
Muscles

Keywords

  • Exercise
  • Hsp90
  • Nitric oxide synthase
  • Skeletal muscle

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Public Health, Environmental and Occupational Health
  • Physiology (medical)

Cite this

Increased nitric oxide synthase activity and Hsp90 association in skeletal muscle following chronic exercise. / Harris, M. Brennan; Mitchell, Brett M.; Sood, Sarika G.; Webb, R Clinton; Venema, Richard C.

In: European Journal of Applied Physiology, Vol. 104, No. 5, 12.09.2008, p. 795-802.

Research output: Contribution to journalArticle

Harris, M. Brennan ; Mitchell, Brett M. ; Sood, Sarika G. ; Webb, R Clinton ; Venema, Richard C. / Increased nitric oxide synthase activity and Hsp90 association in skeletal muscle following chronic exercise. In: European Journal of Applied Physiology. 2008 ; Vol. 104, No. 5. pp. 795-802.
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