Increased oxidative stress and apoptosis in acute puromycin aminonucleoside nephrosis

Jaimar Rincon, Maritza Josefina Romero Lucas, Ninoska Viera, Adriana Pedreañez, Jesus Mosquera

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Accumulating evidence demonstrates that oxidative stress is one of the underlying mechanisms to induce apoptosis in different biological systems. The aim of this study was to examine the simultaneous presence and correlation between oxidative stress events, apoptosis, apoptosis-associated proteins and monocyte/macrophage infiltration during the course of acute puromycin aminonucleoside nephrosis (PAN). To induce nephrosis, Sprague-Dawley rats were injected intraperitoneally with puromycin aminonucleoside and killed at weeks 1 and 2 of nephrosis. Controls represent animals injected with 0.9% saline solution. Kidney sections were homogenized to measure nitric oxide (NO), malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD) and catalase activities by appropriate enzymatic and biochemical methods. Renal frozen sections were studied for superoxide anion (O2-) by a histochemical method, for apoptosis by TUNEL (terminal-deoxynucleotidyl- transferase-mediated dUTP-digoxigenin nick end labelling) and for apoptosis-associated protein expression and monocyte/macrophage infiltration by monoclonal antibodies. Increased renal apoptosis, p53, Bax, Bcl-2 accompanied by increased O2- and NO generation, lipid peroxidation (MDA) and monocyte/macrophage infiltration were found in nephrotic animals. Renal oxidative stress (O2-, NO and MDA) was correlated with apoptosis, p53 expression, monocyte/macrophage cells and proteinuria. Anti-oxidant molecules (SOD and GSH) remained unchanged apart from a decreased activity of catalase which correlated with glomerular apoptosis. In conclusion, the close correlation between the presence of apoptosis and oxidative events confirms the role of oxidative stress in the apoptosis observed during PAN.

Original languageEnglish (US)
Pages (from-to)25-33
Number of pages9
JournalInternational Journal of Experimental Pathology
Volume85
Issue number1
DOIs
StatePublished - Feb 1 2004
Externally publishedYes

Fingerprint

Puromycin Aminonucleoside
Nephrosis
Oxidative Stress
Apoptosis
Monocytes
Malondialdehyde
Macrophages
Kidney
Nitric Oxide
Catalase
Superoxide Dismutase
Digoxigenin
DNA Nucleotidylexotransferase
Frozen Sections
Proteinuria
Oxidants
Sodium Chloride
Superoxides
Lipid Peroxidation
Glutathione

Keywords

  • Aminonucleoside
  • Apoptosis
  • Nephrosis
  • Oxidative stress

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Increased oxidative stress and apoptosis in acute puromycin aminonucleoside nephrosis. / Rincon, Jaimar; Romero Lucas, Maritza Josefina; Viera, Ninoska; Pedreañez, Adriana; Mosquera, Jesus.

In: International Journal of Experimental Pathology, Vol. 85, No. 1, 01.02.2004, p. 25-33.

Research output: Contribution to journalArticle

Rincon, Jaimar ; Romero Lucas, Maritza Josefina ; Viera, Ninoska ; Pedreañez, Adriana ; Mosquera, Jesus. / Increased oxidative stress and apoptosis in acute puromycin aminonucleoside nephrosis. In: International Journal of Experimental Pathology. 2004 ; Vol. 85, No. 1. pp. 25-33.
@article{bd9bcf8f083e43fc94910533019748df,
title = "Increased oxidative stress and apoptosis in acute puromycin aminonucleoside nephrosis",
abstract = "Accumulating evidence demonstrates that oxidative stress is one of the underlying mechanisms to induce apoptosis in different biological systems. The aim of this study was to examine the simultaneous presence and correlation between oxidative stress events, apoptosis, apoptosis-associated proteins and monocyte/macrophage infiltration during the course of acute puromycin aminonucleoside nephrosis (PAN). To induce nephrosis, Sprague-Dawley rats were injected intraperitoneally with puromycin aminonucleoside and killed at weeks 1 and 2 of nephrosis. Controls represent animals injected with 0.9{\%} saline solution. Kidney sections were homogenized to measure nitric oxide (NO), malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD) and catalase activities by appropriate enzymatic and biochemical methods. Renal frozen sections were studied for superoxide anion (O2-) by a histochemical method, for apoptosis by TUNEL (terminal-deoxynucleotidyl- transferase-mediated dUTP-digoxigenin nick end labelling) and for apoptosis-associated protein expression and monocyte/macrophage infiltration by monoclonal antibodies. Increased renal apoptosis, p53, Bax, Bcl-2 accompanied by increased O2- and NO generation, lipid peroxidation (MDA) and monocyte/macrophage infiltration were found in nephrotic animals. Renal oxidative stress (O2-, NO and MDA) was correlated with apoptosis, p53 expression, monocyte/macrophage cells and proteinuria. Anti-oxidant molecules (SOD and GSH) remained unchanged apart from a decreased activity of catalase which correlated with glomerular apoptosis. In conclusion, the close correlation between the presence of apoptosis and oxidative events confirms the role of oxidative stress in the apoptosis observed during PAN.",
keywords = "Aminonucleoside, Apoptosis, Nephrosis, Oxidative stress",
author = "Jaimar Rincon and {Romero Lucas}, {Maritza Josefina} and Ninoska Viera and Adriana Pedrea{\~n}ez and Jesus Mosquera",
year = "2004",
month = "2",
day = "1",
doi = "10.1111/j.0959-9673.2004.0368.x",
language = "English (US)",
volume = "85",
pages = "25--33",
journal = "International Journal of Experimental Pathology",
issn = "0959-9673",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Increased oxidative stress and apoptosis in acute puromycin aminonucleoside nephrosis

AU - Rincon, Jaimar

AU - Romero Lucas, Maritza Josefina

AU - Viera, Ninoska

AU - Pedreañez, Adriana

AU - Mosquera, Jesus

PY - 2004/2/1

Y1 - 2004/2/1

N2 - Accumulating evidence demonstrates that oxidative stress is one of the underlying mechanisms to induce apoptosis in different biological systems. The aim of this study was to examine the simultaneous presence and correlation between oxidative stress events, apoptosis, apoptosis-associated proteins and monocyte/macrophage infiltration during the course of acute puromycin aminonucleoside nephrosis (PAN). To induce nephrosis, Sprague-Dawley rats were injected intraperitoneally with puromycin aminonucleoside and killed at weeks 1 and 2 of nephrosis. Controls represent animals injected with 0.9% saline solution. Kidney sections were homogenized to measure nitric oxide (NO), malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD) and catalase activities by appropriate enzymatic and biochemical methods. Renal frozen sections were studied for superoxide anion (O2-) by a histochemical method, for apoptosis by TUNEL (terminal-deoxynucleotidyl- transferase-mediated dUTP-digoxigenin nick end labelling) and for apoptosis-associated protein expression and monocyte/macrophage infiltration by monoclonal antibodies. Increased renal apoptosis, p53, Bax, Bcl-2 accompanied by increased O2- and NO generation, lipid peroxidation (MDA) and monocyte/macrophage infiltration were found in nephrotic animals. Renal oxidative stress (O2-, NO and MDA) was correlated with apoptosis, p53 expression, monocyte/macrophage cells and proteinuria. Anti-oxidant molecules (SOD and GSH) remained unchanged apart from a decreased activity of catalase which correlated with glomerular apoptosis. In conclusion, the close correlation between the presence of apoptosis and oxidative events confirms the role of oxidative stress in the apoptosis observed during PAN.

AB - Accumulating evidence demonstrates that oxidative stress is one of the underlying mechanisms to induce apoptosis in different biological systems. The aim of this study was to examine the simultaneous presence and correlation between oxidative stress events, apoptosis, apoptosis-associated proteins and monocyte/macrophage infiltration during the course of acute puromycin aminonucleoside nephrosis (PAN). To induce nephrosis, Sprague-Dawley rats were injected intraperitoneally with puromycin aminonucleoside and killed at weeks 1 and 2 of nephrosis. Controls represent animals injected with 0.9% saline solution. Kidney sections were homogenized to measure nitric oxide (NO), malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD) and catalase activities by appropriate enzymatic and biochemical methods. Renal frozen sections were studied for superoxide anion (O2-) by a histochemical method, for apoptosis by TUNEL (terminal-deoxynucleotidyl- transferase-mediated dUTP-digoxigenin nick end labelling) and for apoptosis-associated protein expression and monocyte/macrophage infiltration by monoclonal antibodies. Increased renal apoptosis, p53, Bax, Bcl-2 accompanied by increased O2- and NO generation, lipid peroxidation (MDA) and monocyte/macrophage infiltration were found in nephrotic animals. Renal oxidative stress (O2-, NO and MDA) was correlated with apoptosis, p53 expression, monocyte/macrophage cells and proteinuria. Anti-oxidant molecules (SOD and GSH) remained unchanged apart from a decreased activity of catalase which correlated with glomerular apoptosis. In conclusion, the close correlation between the presence of apoptosis and oxidative events confirms the role of oxidative stress in the apoptosis observed during PAN.

KW - Aminonucleoside

KW - Apoptosis

KW - Nephrosis

KW - Oxidative stress

UR - http://www.scopus.com/inward/record.url?scp=2342453164&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2342453164&partnerID=8YFLogxK

U2 - 10.1111/j.0959-9673.2004.0368.x

DO - 10.1111/j.0959-9673.2004.0368.x

M3 - Article

VL - 85

SP - 25

EP - 33

JO - International Journal of Experimental Pathology

JF - International Journal of Experimental Pathology

SN - 0959-9673

IS - 1

ER -