Increased telomerase activity and vitamin D supplementation in overweight African Americans

Haidong Zhu, D. Guo, K. Li, J. Pedersen-White, I. S. Stallmann-Jorgensen, Y. Huang, S. Parikh, Kebin Liu, Yanbin Dong

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Objective: We aimed to investigate whether vitamin D supplementation modulates peripheral blood mononuclear cell (PBMC) telomerase activity in overweight African Americans. Design: A double blind, randomized and placebo-controlled clinical trial (NCT01141192) was recently conducted.Subjects And Methods:African-American adults were randomly assigned to either the placebo, or the vitamin D group (60 000 IU per month (equivalent to ∼ 2000 IU per day) oral vitamin D3 supplementation). Fresh PBMCs were collected from 37 subjects (18 in the placebo group and 19 in the vitamin D group), both at baseline and 16 weeks. PBMC telomerase activity was measured by the telomeric repeat amplification protocol. Results: Serum 25 hydroxyvitamin D levels increased from 40.7±15.7 at baseline to 48.1±17.5 nmol l -1 at posttest (P<0.004) in the placebo group, and from 35.411.3 at baseline to 103.731.5 nmol l-1 at posttests (P<0.0001) in the vitamin D group. In the vitamin D group, PBMC telomerase activity increased by 19.2% from baseline (1.560.29 absorbance reading unit (AU)) to posttest (1.860.42 AU, P<0.0001). The significance persisted after controlling for age, sex and body mass index (P<0.039). PBMC telomerase activity in the placebo group did not change from baseline (1.430.26 AU) to posttest (1.460.27 AU, P<0.157). Conclusion: Vitamin D supplementation significantly increased PBMC telomerase activity in overweight African Americans. Our data suggest that vitamin D may improve telomere maintenance and prevent cell senescence and counteract obesity-induced acceleration of cellular aging.

Original languageEnglish (US)
Pages (from-to)805-809
Number of pages5
JournalInternational Journal of Obesity
Volume36
Issue number6
DOIs
StatePublished - Jun 1 2012

Fingerprint

Telomerase
Vitamin D
African Americans
Blood Cells
Placebos
Reading
Cell Aging
Cholecalciferol
Telomere
Body Mass Index
Randomized Controlled Trials
Obesity
Maintenance
Serum

Keywords

  • 25(OH)D
  • African Americans
  • telomerase activity
  • vitamin D supplementation

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Increased telomerase activity and vitamin D supplementation in overweight African Americans. / Zhu, Haidong; Guo, D.; Li, K.; Pedersen-White, J.; Stallmann-Jorgensen, I. S.; Huang, Y.; Parikh, S.; Liu, Kebin; Dong, Yanbin.

In: International Journal of Obesity, Vol. 36, No. 6, 01.06.2012, p. 805-809.

Research output: Contribution to journalArticle

Zhu, H, Guo, D, Li, K, Pedersen-White, J, Stallmann-Jorgensen, IS, Huang, Y, Parikh, S, Liu, K & Dong, Y 2012, 'Increased telomerase activity and vitamin D supplementation in overweight African Americans', International Journal of Obesity, vol. 36, no. 6, pp. 805-809. https://doi.org/10.1038/ijo.2011.197
Zhu, Haidong ; Guo, D. ; Li, K. ; Pedersen-White, J. ; Stallmann-Jorgensen, I. S. ; Huang, Y. ; Parikh, S. ; Liu, Kebin ; Dong, Yanbin. / Increased telomerase activity and vitamin D supplementation in overweight African Americans. In: International Journal of Obesity. 2012 ; Vol. 36, No. 6. pp. 805-809.
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AB - Objective: We aimed to investigate whether vitamin D supplementation modulates peripheral blood mononuclear cell (PBMC) telomerase activity in overweight African Americans. Design: A double blind, randomized and placebo-controlled clinical trial (NCT01141192) was recently conducted.Subjects And Methods:African-American adults were randomly assigned to either the placebo, or the vitamin D group (60 000 IU per month (equivalent to ∼ 2000 IU per day) oral vitamin D3 supplementation). Fresh PBMCs were collected from 37 subjects (18 in the placebo group and 19 in the vitamin D group), both at baseline and 16 weeks. PBMC telomerase activity was measured by the telomeric repeat amplification protocol. Results: Serum 25 hydroxyvitamin D levels increased from 40.7±15.7 at baseline to 48.1±17.5 nmol l -1 at posttest (P<0.004) in the placebo group, and from 35.411.3 at baseline to 103.731.5 nmol l-1 at posttests (P<0.0001) in the vitamin D group. In the vitamin D group, PBMC telomerase activity increased by 19.2% from baseline (1.560.29 absorbance reading unit (AU)) to posttest (1.860.42 AU, P<0.0001). The significance persisted after controlling for age, sex and body mass index (P<0.039). PBMC telomerase activity in the placebo group did not change from baseline (1.430.26 AU) to posttest (1.460.27 AU, P<0.157). Conclusion: Vitamin D supplementation significantly increased PBMC telomerase activity in overweight African Americans. Our data suggest that vitamin D may improve telomere maintenance and prevent cell senescence and counteract obesity-induced acceleration of cellular aging.

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