Increaseed expression of mRNA for regulator of G protein signaling domain-containing Rho guanine nucleotide exchange factors in aorta from stroke-prone spontaneously hypertensive rats

Zhekang Ying, Liming Jin, Anne M. Dorrance, R Clinton Webb

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44 Citations (Scopus)

Abstract

Rho guanine nucleotide exchange factors (RhoGEF) link activation of G protein-coupled receptors (GPCR) to RhoA/Rho-kinase signaling. This cellular signaling pathway regulates vascular tone and is implicated in hypertension. The RhoGEF, providing this coupling, contain the regulator of G protein signaling (RGS) domain. We hypothesized higher mRNA expression levels for these RhoGEF in aorta from stroke-prone spontaneously hypertensive rats (SHRSP). We measured mRNA expression by semiquantitative reverse transcriptase-polymerase chain reaction in the aorta of Wistar-Kyoto rats (WKY) and SHRSP. The protein expression level of p115RhoGEF was examined by Western Blot, too. Our results showed that mRNA for RGS domain containing RhoGEF is higher in SHRSP. The results of this study indicate that RhoGEF may contribute to increased activation of RhoA/Rho-kinase pathway in hypertension.

Original languageEnglish (US)
Pages (from-to)981-985
Number of pages5
JournalAmerican Journal of Hypertension
Volume17
Issue number10
DOIs
StatePublished - Oct 1 2004

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Rho Guanine Nucleotide Exchange Factors
Inbred SHR Rats
GTP-Binding Proteins
Aorta
Stroke
Messenger RNA
rho-Associated Kinases
Hypertension
Inbred WKY Rats
G-Protein-Coupled Receptors
Reverse Transcriptase Polymerase Chain Reaction
Blood Vessels
Protein Domains
Western Blotting

Keywords

  • G protein coupled receptor
  • Rho guanine nucleotide exchange factors
  • RhoA
  • regulator of G protein signaling domain containing
  • stroke-prone spontaneously hypertensive rat
  • vascular smooth muscle

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

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title = "Increaseed expression of mRNA for regulator of G protein signaling domain-containing Rho guanine nucleotide exchange factors in aorta from stroke-prone spontaneously hypertensive rats",
abstract = "Rho guanine nucleotide exchange factors (RhoGEF) link activation of G protein-coupled receptors (GPCR) to RhoA/Rho-kinase signaling. This cellular signaling pathway regulates vascular tone and is implicated in hypertension. The RhoGEF, providing this coupling, contain the regulator of G protein signaling (RGS) domain. We hypothesized higher mRNA expression levels for these RhoGEF in aorta from stroke-prone spontaneously hypertensive rats (SHRSP). We measured mRNA expression by semiquantitative reverse transcriptase-polymerase chain reaction in the aorta of Wistar-Kyoto rats (WKY) and SHRSP. The protein expression level of p115RhoGEF was examined by Western Blot, too. Our results showed that mRNA for RGS domain containing RhoGEF is higher in SHRSP. The results of this study indicate that RhoGEF may contribute to increased activation of RhoA/Rho-kinase pathway in hypertension.",
keywords = "G protein coupled receptor, Rho guanine nucleotide exchange factors, RhoA, regulator of G protein signaling domain containing, stroke-prone spontaneously hypertensive rat, vascular smooth muscle",
author = "Zhekang Ying and Liming Jin and Dorrance, {Anne M.} and Webb, {R Clinton}",
year = "2004",
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T1 - Increaseed expression of mRNA for regulator of G protein signaling domain-containing Rho guanine nucleotide exchange factors in aorta from stroke-prone spontaneously hypertensive rats

AU - Ying, Zhekang

AU - Jin, Liming

AU - Dorrance, Anne M.

AU - Webb, R Clinton

PY - 2004/10/1

Y1 - 2004/10/1

N2 - Rho guanine nucleotide exchange factors (RhoGEF) link activation of G protein-coupled receptors (GPCR) to RhoA/Rho-kinase signaling. This cellular signaling pathway regulates vascular tone and is implicated in hypertension. The RhoGEF, providing this coupling, contain the regulator of G protein signaling (RGS) domain. We hypothesized higher mRNA expression levels for these RhoGEF in aorta from stroke-prone spontaneously hypertensive rats (SHRSP). We measured mRNA expression by semiquantitative reverse transcriptase-polymerase chain reaction in the aorta of Wistar-Kyoto rats (WKY) and SHRSP. The protein expression level of p115RhoGEF was examined by Western Blot, too. Our results showed that mRNA for RGS domain containing RhoGEF is higher in SHRSP. The results of this study indicate that RhoGEF may contribute to increased activation of RhoA/Rho-kinase pathway in hypertension.

AB - Rho guanine nucleotide exchange factors (RhoGEF) link activation of G protein-coupled receptors (GPCR) to RhoA/Rho-kinase signaling. This cellular signaling pathway regulates vascular tone and is implicated in hypertension. The RhoGEF, providing this coupling, contain the regulator of G protein signaling (RGS) domain. We hypothesized higher mRNA expression levels for these RhoGEF in aorta from stroke-prone spontaneously hypertensive rats (SHRSP). We measured mRNA expression by semiquantitative reverse transcriptase-polymerase chain reaction in the aorta of Wistar-Kyoto rats (WKY) and SHRSP. The protein expression level of p115RhoGEF was examined by Western Blot, too. Our results showed that mRNA for RGS domain containing RhoGEF is higher in SHRSP. The results of this study indicate that RhoGEF may contribute to increased activation of RhoA/Rho-kinase pathway in hypertension.

KW - G protein coupled receptor

KW - Rho guanine nucleotide exchange factors

KW - RhoA

KW - regulator of G protein signaling domain containing

KW - stroke-prone spontaneously hypertensive rat

KW - vascular smooth muscle

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