Indole-3-carbinol improves survival in lupus-prone mice by inducing tandem B- and T-cell differentiation blockades

Xiao jie Yan, Mei Qi, Gloria Telusma, Sophia Yancopoulos, Michael P. Madaio, Minoru Satoh, Westley H. Reeves, Saul Teichberg, Nina Kohn, Karen Auborn, Nicholas Chiorazzi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Indole-3-carbinol (I3C), derived from cruciferous vegetables, alters estrogen metabolism. Since lupus is estrogen dependent, we reasoned that I3C might be effective in SLE. I3C significantly thwarted disease progression and prolonged survival in (NZB × NZW) F1 mice. Immunofluorescent and serologic analyses in treated animals indicated a transient blockade in B-cell maturation with increased immature B cells, decreased mature B cells, and a significant reduction of certain autoantibodies. Subsequently, a delay in T-cell maturation occurred in the treated group, manifested by significantly increased naive T cells, decreased mature and memory T cells, and decreased CD4:CD8 T-cell ratios. T cells from the I3C cohort, stimulated in vitro with various mitogens, exhibited enhanced responsiveness. Con A-stimulated T cells from I3C-treated mice produced Th1 cytokines, whereas those from control animals produced Th2 cytokines. Our studies suggest immunological mechanisms by which I3C ameliorates SLE in mice and provide a rationale for its use as an adjunctive therapy for human lupus.

Original languageEnglish (US)
Pages (from-to)481-494
Number of pages14
JournalClinical Immunology
Volume131
Issue number3
DOIs
StatePublished - Jun 1 2009

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Cell Differentiation
T-Lymphocytes
Survival
Estrogens
B-Lymphocytes
Cytokines
B-Lymphoid Precursor Cells
Mitogens
Vegetables
Autoantibodies
Disease Progression
indole-3-carbinol
Therapeutics

Keywords

  • Autoantibodies
  • B lymphocytes
  • Cytokines
  • Estrogen
  • I3C
  • Lymphocyte maturation
  • Renal disease
  • SLE
  • Systemic lupus erythematosus
  • T lymphocytes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Yan, X. J., Qi, M., Telusma, G., Yancopoulos, S., Madaio, M. P., Satoh, M., ... Chiorazzi, N. (2009). Indole-3-carbinol improves survival in lupus-prone mice by inducing tandem B- and T-cell differentiation blockades. Clinical Immunology, 131(3), 481-494. https://doi.org/10.1016/j.clim.2009.01.013

Indole-3-carbinol improves survival in lupus-prone mice by inducing tandem B- and T-cell differentiation blockades. / Yan, Xiao jie; Qi, Mei; Telusma, Gloria; Yancopoulos, Sophia; Madaio, Michael P.; Satoh, Minoru; Reeves, Westley H.; Teichberg, Saul; Kohn, Nina; Auborn, Karen; Chiorazzi, Nicholas.

In: Clinical Immunology, Vol. 131, No. 3, 01.06.2009, p. 481-494.

Research output: Contribution to journalArticle

Yan, XJ, Qi, M, Telusma, G, Yancopoulos, S, Madaio, MP, Satoh, M, Reeves, WH, Teichberg, S, Kohn, N, Auborn, K & Chiorazzi, N 2009, 'Indole-3-carbinol improves survival in lupus-prone mice by inducing tandem B- and T-cell differentiation blockades', Clinical Immunology, vol. 131, no. 3, pp. 481-494. https://doi.org/10.1016/j.clim.2009.01.013
Yan, Xiao jie ; Qi, Mei ; Telusma, Gloria ; Yancopoulos, Sophia ; Madaio, Michael P. ; Satoh, Minoru ; Reeves, Westley H. ; Teichberg, Saul ; Kohn, Nina ; Auborn, Karen ; Chiorazzi, Nicholas. / Indole-3-carbinol improves survival in lupus-prone mice by inducing tandem B- and T-cell differentiation blockades. In: Clinical Immunology. 2009 ; Vol. 131, No. 3. pp. 481-494.
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