Inducible nitric oxide synthase and the effect of aminoguanidine in experimental neonatal meningitis

Stephen L. Leib, Young S. Km, Stephen Matthew Black, Jay H. Tureen, Martin G. Täuber

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

This study explored the role of inducible nitric oxide (NO) synthase (iNOS) in an infant rat model of group B streptococcal meningitis. Brain iNOS activity increased during meningitis (P < .001), and iNOS was detected by immunocytochemistry in the walls of meningeal vessels and cells of the cerebrospinal fluid (CSF) inflammation. Animals treated with iNOS inhibitor aminoguanidine (AG; 130 mg/kg every 8 h) had reduced NO production (P < .05), higher CSF bacterial titers (P < .05), and increased incidence of seizures (P < .01) compared with untreated infected animals. AG also increased areas of severe hypoperfusion in the cortex (31% ± 14% in controls vs. 56% ± 16% in AG; P < .01) and the extent of cortical neuronal injury, both when administered at the time of infection (P < .05) and in established meningitis (P < .02). Thus, NO produced by iNOS may be beneficial in this model of experimental meningitis by reducing cerebral ischemia.

Original languageEnglish (US)
Pages (from-to)692-700
Number of pages9
JournalJournal of Infectious Diseases
Volume177
Issue number3
DOIs
StatePublished - Jan 1 1998

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Nitric Oxide Synthase Type II
Meningitis
Cerebrospinal Fluid
Nitric Oxide
Brain Ischemia
Seizures
Theoretical Models
Immunohistochemistry
Inflammation
pimagedine
Incidence
Wounds and Injuries
Brain
Infection

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Inducible nitric oxide synthase and the effect of aminoguanidine in experimental neonatal meningitis. / Leib, Stephen L.; Km, Young S.; Black, Stephen Matthew; Tureen, Jay H.; Täuber, Martin G.

In: Journal of Infectious Diseases, Vol. 177, No. 3, 01.01.1998, p. 692-700.

Research output: Contribution to journalArticle

Leib, Stephen L. ; Km, Young S. ; Black, Stephen Matthew ; Tureen, Jay H. ; Täuber, Martin G. / Inducible nitric oxide synthase and the effect of aminoguanidine in experimental neonatal meningitis. In: Journal of Infectious Diseases. 1998 ; Vol. 177, No. 3. pp. 692-700.
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