Induction of apoptosis by Cordyceps militaris through activation of caspase-3 in leukemia HL-60 cells

Haemi Lee, Yun Jung Kim, Ha Won Kim, Dong Hee Lee, Mi Kyung Sung, Taesun Park

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Cordyceps militaris is a traditional herbal ingredient frequently used for tonic and medicinal purposes in eastern Asia. The hot water extract of its cultivated fruiting bodies demonstrated a potent cytotoxic effect against the proliferation of the human premyelocytic leukemia cell HL-60, with an IC 50 of 0.8 mg/ml for a 12-h treatment. It induced the characteristic apoptotic symptoms in the HL-60 cells, including DNA fragmentation and chromatin condensation, occurring within 12 - 16 h of treatment at a dose of 1 mg/ml. The activation of caspase-3 and the specific proteolytic cleavage of poly (ADP-ribose) polymerase were detected during the course of apoptosis induction. These results indicate that the hot water extract of Cordyceps militaris fruiting bodies inhibited cancer cell proliferation by inducing cell apoptosis through the activation of caspase-3, and that the Cordyceps militaris extract may therefore have therapeutic potential against human leukemia.

Original languageEnglish (US)
Pages (from-to)670-674
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume29
Issue number4
DOIs
StatePublished - Apr 1 2006
Externally publishedYes

Keywords

  • Apoptosis
  • Caspase-3
  • Cordyceps militaris
  • Cytochrome c
  • HL-60
  • Poly (ADP-ribose) polymerase (PARP)

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Fingerprint Dive into the research topics of 'Induction of apoptosis by Cordyceps militaris through activation of caspase-3 in leukemia HL-60 cells'. Together they form a unique fingerprint.

  • Cite this