TY - JOUR
T1 - Induction of CRP3/MLP expression during vein arterialization is dependent on stretch rather than shear stress
AU - Campos, Luciene Cristina Gastalho
AU - Miyakawa, Ayumi Aurea
AU - Barauna, Valerio Garrone
AU - Cardoso, Leandro
AU - Borin, Thaiz Ferraz
AU - Dallan, Luis Alberto De Oliveira
AU - Krieger, Jose Eduardo
N1 - Funding Information:
This work was supported by Fundacao de Amparo a Pesquisa do Estado de Sao Paulo—FAPESP [01/00009-0] and Conselho Nacional de Desenvolvimento Cientifico e Tecnológico— CNPq [478073/2004-6]. L.C.G.C., A.A.M., and V.G.B. are recipient of fellowship from FAPESP—(03/14115-2, 00/ 09485-7, 06/52053-7, respectively).
PY - 2009/7
Y1 - 2009/7
N2 - Aims: Cysteine- and glycine-rich protein 3/muscle LIM-domain protein (CRP3/MLP) mediates protein-protein interaction with actin filaments in the heart and is involved in muscle differentiation and vascular remodelling. Here, we assessed the induction of CRP3/MLP expression during arterialization in human and rat veins. Methods and results: Vascular CRP3/MLP expression was mainly observed in arterial samples from both human and rat. Using quantitative real time RT-PCR, we demonstrated that the CRP3/MLP expression was 10 times higher in smooth muscle cells (SMCs) from human mammary artery (h-MA) vs. saphenous vein (h-SV). In endothelial cells (ECs), CRP3/MLP was scarcely detected in either h-MA or h-SV. Using an ex vivo flow through system that mimics arterial condition, we observed induction of CRP3/MLP expression in arterialized h-SV. Interestingly, the upregulation of CRP3/MLP was primarily dependent on stretch stimulus in SMCs, rather than shear stress in ECs. Finally, using a rat vein in vivo arterialization model, early (1-14 days) CRP3/MLP immunostaining was observed predominantly in the inner layer and later (28-90 days) it appeared more scattered in the vessel layers. Conclusion: Here we provide evidence that CRP3/MLP is primarily expressed in arterial SMCs and that stretch is the main stimulus for CRP3/MLP induction in veins exposed to arterial haemodynamic conditions.
AB - Aims: Cysteine- and glycine-rich protein 3/muscle LIM-domain protein (CRP3/MLP) mediates protein-protein interaction with actin filaments in the heart and is involved in muscle differentiation and vascular remodelling. Here, we assessed the induction of CRP3/MLP expression during arterialization in human and rat veins. Methods and results: Vascular CRP3/MLP expression was mainly observed in arterial samples from both human and rat. Using quantitative real time RT-PCR, we demonstrated that the CRP3/MLP expression was 10 times higher in smooth muscle cells (SMCs) from human mammary artery (h-MA) vs. saphenous vein (h-SV). In endothelial cells (ECs), CRP3/MLP was scarcely detected in either h-MA or h-SV. Using an ex vivo flow through system that mimics arterial condition, we observed induction of CRP3/MLP expression in arterialized h-SV. Interestingly, the upregulation of CRP3/MLP was primarily dependent on stretch stimulus in SMCs, rather than shear stress in ECs. Finally, using a rat vein in vivo arterialization model, early (1-14 days) CRP3/MLP immunostaining was observed predominantly in the inner layer and later (28-90 days) it appeared more scattered in the vessel layers. Conclusion: Here we provide evidence that CRP3/MLP is primarily expressed in arterial SMCs and that stretch is the main stimulus for CRP3/MLP induction in veins exposed to arterial haemodynamic conditions.
KW - Arterialized vein graft
KW - CRP3/MLP
KW - Myocardial revascularization
KW - Saphenous vein
KW - Stretch
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U2 - 10.1093/cvr/cvp108
DO - 10.1093/cvr/cvp108
M3 - Article
C2 - 19351738
AN - SCOPUS:67649324907
SN - 0008-6363
VL - 83
SP - 140
EP - 147
JO - Cardiovascular Research
JF - Cardiovascular Research
IS - 1
ER -