Induction of CRP3/MLP expression during vein arterialization is dependent on stretch rather than shear stress

Luciene Cristina Gastalho Campos, Ayumi Aurea Miyakawa, Valerio Garrone Barauna, Leandro Cardoso, Thaiz Ferraz Borin, Luis Alberto De Oliveira Dallan, Jose Eduardo Krieger

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Aims: Cysteine- and glycine-rich protein 3/muscle LIM-domain protein (CRP3/MLP) mediates protein-protein interaction with actin filaments in the heart and is involved in muscle differentiation and vascular remodelling. Here, we assessed the induction of CRP3/MLP expression during arterialization in human and rat veins. Methods and results: Vascular CRP3/MLP expression was mainly observed in arterial samples from both human and rat. Using quantitative real time RT-PCR, we demonstrated that the CRP3/MLP expression was 10 times higher in smooth muscle cells (SMCs) from human mammary artery (h-MA) vs. saphenous vein (h-SV). In endothelial cells (ECs), CRP3/MLP was scarcely detected in either h-MA or h-SV. Using an ex vivo flow through system that mimics arterial condition, we observed induction of CRP3/MLP expression in arterialized h-SV. Interestingly, the upregulation of CRP3/MLP was primarily dependent on stretch stimulus in SMCs, rather than shear stress in ECs. Finally, using a rat vein in vivo arterialization model, early (1-14 days) CRP3/MLP immunostaining was observed predominantly in the inner layer and later (28-90 days) it appeared more scattered in the vessel layers. Conclusion: Here we provide evidence that CRP3/MLP is primarily expressed in arterial SMCs and that stretch is the main stimulus for CRP3/MLP induction in veins exposed to arterial haemodynamic conditions.

Original languageEnglish (US)
Pages (from-to)140-147
Number of pages8
JournalCardiovascular Research
Volume83
Issue number1
DOIs
StatePublished - Jul 2009

Keywords

  • Arterialized vein graft
  • CRP3/MLP
  • Myocardial revascularization
  • Saphenous vein
  • Stretch

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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    Campos, L. C. G., Miyakawa, A. A., Barauna, V. G., Cardoso, L., Borin, T. F., Dallan, L. A. D. O., & Krieger, J. E. (2009). Induction of CRP3/MLP expression during vein arterialization is dependent on stretch rather than shear stress. Cardiovascular Research, 83(1), 140-147. https://doi.org/10.1093/cvr/cvp108