Induction of dectin-1 expression and intracellular signal transduction by β-Glucan of ganoderma lucidum

Han Wook Ryu, Ha Won Kim

Research output: Contribution to journalArticle

Abstract

Fungal β-glucan, known to have immunostimulatory and antitumor activities, can be recognized by host immune cells as one of the pathogen-associated molecular patterns (PAMPs). Although there are several reports on the diverse immunostimulatory activities of β-glucan, little is known about the intracellular signal transduction of β-glucan. Stimulation of RAW264.7 macrophage cells with β-glucan from Ganoderma lucidum induced the expressions of dectin-1, toll-like receptor 2 (TLR2), TLR4, and TLR6 at the transcription stage. Treatment with β-glucan also induced inflammatory mediators such as macrophage inflammatory proteins (MIP)-1α, MIP-1β, MIP-1γ, interleukin (IL)-1β, and tumor necrosis factor (TNF)-α. Treatment of the cells with polymyxin B, an inhibitor of lipopolysaccharides (LPS), blocked the induction of inflammatory mediators in LPS-or β-glucan-stimulated systems. Pretreatment of the cells in our cell culture system with LY294002, a phosphoinositide 3-kinase (PI3K) inhibitor, or U0126, a mitogen-activated protein kinase/extracellular-signal-regulated kinase (MAPK/ERK) kinase (MEK)1/MEK2 inhibitor, led to a reduction in the induction of inflammatory mediators in a concentration-dependent manner. These results show that stimulation of the macrophage cells by β-glucan induced the expressions of both dectin-1 and TLRs. We also found that the PI3K/Akt and MEK pathways were involved in the induction of inflammatory mediators in macrophage cells during intracellular signal transduction of β-glucan.

Original languageEnglish (US)
Pages (from-to)161-176
Number of pages16
JournalKorean Journal of Mycology
Volume46
Issue number2
DOIs
StatePublished - Jun 1 2018
Externally publishedYes

Fingerprint

Ganoderma lucidum
glucans
signal transduction
inhibitor
protein
mitogen-activated protein kinase
macrophages
phosphatidylinositol 3-kinase
cells
tumor
lipopolysaccharides
phosphotransferases (kinases)
pathogen
polymyxin B
tumor necrosis factors
interleukin-1
cell culture
pretreatment
pathogens

Keywords

  • Dectin-1
  • Ganoderma lucidum
  • MIP-1
  • TNF-α
  • β-Glucan

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Plant Science

Cite this

Induction of dectin-1 expression and intracellular signal transduction by β-Glucan of ganoderma lucidum. / Ryu, Han Wook; Kim, Ha Won.

In: Korean Journal of Mycology, Vol. 46, No. 2, 01.06.2018, p. 161-176.

Research output: Contribution to journalArticle

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