Induction of experimental autoimmune encephalomyelitis in guinea pigs using myelin basic protein and myelin glycolipids

Kusunoki Susumu Kusunoki, K. Yu Robert, Jung H. Kim

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Strain 13 guinea pigs were immunized with galactocerebroside, asialo-GM1 (GA1) or GM4 ganglioside in association with myelin basic protein (MBP) in complete Freund's adjuvant (CFA) to produce experimental autoimmune encephalomyelitis (EAE). The clinical and pathological features, serum antibodies, and lipid compositions of affected brains and spinal cords were compared with those of guinea pigs immunized with MBP, in CFA, alone. Perivascular demyelination was seen in brains from all guinea pigs immunized with GA1/MBP. The incidence and degree of demyelination in this group were significantly higher than in the group immunized with only MBP. The onset of EAE was slightly, but significantly, retarded in groups of animals immunized with GM4/MBP and there was no detectable demyelination. Otherwise, no significant differences were detected between groups. Augmentation of EAE by myelin glycolipids may provide some important clues in understanding the mechanism of demyelinating diseases.

Original languageEnglish (US)
Pages (from-to)303-314
Number of pages12
JournalJournal of Neuroimmunology
Volume18
Issue number4
DOIs
StatePublished - Jul 1988
Externally publishedYes

Keywords

  • (Guinea pig)
  • Experimental autoimmune encephalomyelitis
  • Myelin basic protein
  • Myelin glycolipid

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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