TY - JOUR
T1 - Induction of experimental autoimmune encephalomyelitis in guinea pigs using myelin basic protein and myelin glycolipids
AU - Susumu Kusunoki, Kusunoki
AU - Robert, K. Yu
AU - Kim, Jung H.
N1 - Funding Information:
The authors gratefully acknowledge Dr. C.S. Raine for providing animals used in this investigation and for reviewing the pathological slides. Financial assistance was provided by USPHS grants NS-11853, NS-23102 and by a National Multiple Sclerosis Society grant RG 1950-A-5.
PY - 1988/7
Y1 - 1988/7
N2 - Strain 13 guinea pigs were immunized with galactocerebroside, asialo-GM1 (GA1) or GM4 ganglioside in association with myelin basic protein (MBP) in complete Freund's adjuvant (CFA) to produce experimental autoimmune encephalomyelitis (EAE). The clinical and pathological features, serum antibodies, and lipid compositions of affected brains and spinal cords were compared with those of guinea pigs immunized with MBP, in CFA, alone. Perivascular demyelination was seen in brains from all guinea pigs immunized with GA1/MBP. The incidence and degree of demyelination in this group were significantly higher than in the group immunized with only MBP. The onset of EAE was slightly, but significantly, retarded in groups of animals immunized with GM4/MBP and there was no detectable demyelination. Otherwise, no significant differences were detected between groups. Augmentation of EAE by myelin glycolipids may provide some important clues in understanding the mechanism of demyelinating diseases.
AB - Strain 13 guinea pigs were immunized with galactocerebroside, asialo-GM1 (GA1) or GM4 ganglioside in association with myelin basic protein (MBP) in complete Freund's adjuvant (CFA) to produce experimental autoimmune encephalomyelitis (EAE). The clinical and pathological features, serum antibodies, and lipid compositions of affected brains and spinal cords were compared with those of guinea pigs immunized with MBP, in CFA, alone. Perivascular demyelination was seen in brains from all guinea pigs immunized with GA1/MBP. The incidence and degree of demyelination in this group were significantly higher than in the group immunized with only MBP. The onset of EAE was slightly, but significantly, retarded in groups of animals immunized with GM4/MBP and there was no detectable demyelination. Otherwise, no significant differences were detected between groups. Augmentation of EAE by myelin glycolipids may provide some important clues in understanding the mechanism of demyelinating diseases.
KW - (Guinea pig)
KW - Experimental autoimmune encephalomyelitis
KW - Myelin basic protein
KW - Myelin glycolipid
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U2 - 10.1016/0165-5728(88)90051-3
DO - 10.1016/0165-5728(88)90051-3
M3 - Article
C2 - 2454944
AN - SCOPUS:0023907975
VL - 18
SP - 303
EP - 314
JO - Advances in Neuroimmunology
JF - Advances in Neuroimmunology
SN - 0165-5728
IS - 4
ER -