Abstract
Strain 13 guinea pigs were immunized with galactocerebroside, asialo-GM1 (GA1) or GM4 ganglioside in association with myelin basic protein (MBP) in complete Freund's adjuvant (CFA) to produce experimental autoimmune encephalomyelitis (EAE). The clinical and pathological features, serum antibodies, and lipid compositions of affected brains and spinal cords were compared with those of guinea pigs immunized with MBP, in CFA, alone. Perivascular demyelination was seen in brains from all guinea pigs immunized with GA1/MBP. The incidence and degree of demyelination in this group were significantly higher than in the group immunized with only MBP. The onset of EAE was slightly, but significantly, retarded in groups of animals immunized with GM4/MBP and there was no detectable demyelination. Otherwise, no significant differences were detected between groups. Augmentation of EAE by myelin glycolipids may provide some important clues in understanding the mechanism of demyelinating diseases.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 303-314 |
| Number of pages | 12 |
| Journal | Journal of Neuroimmunology |
| Volume | 18 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jul 1988 |
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Keywords
- (Guinea pig)
- Experimental autoimmune encephalomyelitis
- Myelin basic protein
- Myelin glycolipid
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Neurology
- Clinical Neurology
Cite this
Induction of experimental autoimmune encephalomyelitis in guinea pigs using myelin basic protein and myelin glycolipids. / Susumu Kusunoki, Kusunoki; Robert, K. Yu; Kim, Jung H.
In: Journal of Neuroimmunology, Vol. 18, No. 4, 07.1988, p. 303-314.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Induction of experimental autoimmune encephalomyelitis in guinea pigs using myelin basic protein and myelin glycolipids
AU - Susumu Kusunoki, Kusunoki
AU - Robert, K. Yu
AU - Kim, Jung H.
PY - 1988/7
Y1 - 1988/7
N2 - Strain 13 guinea pigs were immunized with galactocerebroside, asialo-GM1 (GA1) or GM4 ganglioside in association with myelin basic protein (MBP) in complete Freund's adjuvant (CFA) to produce experimental autoimmune encephalomyelitis (EAE). The clinical and pathological features, serum antibodies, and lipid compositions of affected brains and spinal cords were compared with those of guinea pigs immunized with MBP, in CFA, alone. Perivascular demyelination was seen in brains from all guinea pigs immunized with GA1/MBP. The incidence and degree of demyelination in this group were significantly higher than in the group immunized with only MBP. The onset of EAE was slightly, but significantly, retarded in groups of animals immunized with GM4/MBP and there was no detectable demyelination. Otherwise, no significant differences were detected between groups. Augmentation of EAE by myelin glycolipids may provide some important clues in understanding the mechanism of demyelinating diseases.
AB - Strain 13 guinea pigs were immunized with galactocerebroside, asialo-GM1 (GA1) or GM4 ganglioside in association with myelin basic protein (MBP) in complete Freund's adjuvant (CFA) to produce experimental autoimmune encephalomyelitis (EAE). The clinical and pathological features, serum antibodies, and lipid compositions of affected brains and spinal cords were compared with those of guinea pigs immunized with MBP, in CFA, alone. Perivascular demyelination was seen in brains from all guinea pigs immunized with GA1/MBP. The incidence and degree of demyelination in this group were significantly higher than in the group immunized with only MBP. The onset of EAE was slightly, but significantly, retarded in groups of animals immunized with GM4/MBP and there was no detectable demyelination. Otherwise, no significant differences were detected between groups. Augmentation of EAE by myelin glycolipids may provide some important clues in understanding the mechanism of demyelinating diseases.
KW - (Guinea pig)
KW - Experimental autoimmune encephalomyelitis
KW - Myelin basic protein
KW - Myelin glycolipid
UR - http://www.scopus.com/inward/record.url?scp=0023907975&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023907975&partnerID=8YFLogxK
U2 - 10.1016/0165-5728(88)90051-3
DO - 10.1016/0165-5728(88)90051-3
M3 - Article
C2 - 2454944
AN - SCOPUS:0023907975
VL - 18
SP - 303
EP - 314
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
SN - 0165-5728
IS - 4
ER -