Induction of host chemotactic response by Encephalitozoon spp.

Jeffrey L Fischer, Jeffrey West, Nnenaya Agochukwu, Colby Suire, Hollie Hale-Donze

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Microsporidians are a group of emerging pathogens typically associated with chronic diarrhea in immunocompromised individuals. The number of reports of infections with these organisms and the disseminated pathology is growing as diagnostic tools become more readily available. However, little is known about the innate immune response induced by and generated against these parasites. Using a coculture chemotaxis system, primary human macrophages were infected with Encephalitozoon cuniculi or Encephalitozoon intestinalis, and the recruitment of naïve monocytes was monitored. Encephalitozoon spp. induced an average threefold increase in migration of naïve cells 48 h postinfection, which corresponded to optimal infection of monocyte-derived- macrophages. A limited microarray analysis of infected macrophages revealed several chemokines involved in the inflammatory responses whose expression was upregulated, including CCL1, CCL2, CCL3, CCL4, CCL7, CCL15, CCL20, CXCLl, CXCL2, CXCL3, CXCLS, and CXCL8. The levels of 6 of 11 chemokines also present in the microarray were confirmed to be elevated by protein profiling. Kinetic studies confirmed that secreted CCL2, CCL3, and CCL4 were expressed as early as 6 h postinfection, with peak expression at 12 to 24 h and expression remaining until 48 h postinfection. Neutralization of these chemokines, specifically CCL4, significantly reduced the number of migrating cells in vitro, indicating their role in the induction of monocyte migration. This mechanism of recruitment not only supports the evidence that in vivo cellular infiltration occurs but also provides new hosts for the parasites, which escape macrophages by rupturing the host cell. To our knowledge, this is the first documentation that chemokine production is induced by microsporidian infections in human macrophages.

Original languageEnglish (US)
Pages (from-to)1619-1625
Number of pages7
JournalInfection and Immunity
Volume75
Issue number4
DOIs
StatePublished - Apr 1 2007

Fingerprint

Encephalitozoon
Macrophages
Chemokines
Monocytes
Parasites
Encephalitozoon cuniculi
Infection
Chemokine CCL4
Chemotaxis
Microarray Analysis
Coculture Techniques
Innate Immunity
Documentation
Cell Movement
Diarrhea
Cell Count
Pathology
Proteins

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Induction of host chemotactic response by Encephalitozoon spp. / Fischer, Jeffrey L; West, Jeffrey; Agochukwu, Nnenaya; Suire, Colby; Hale-Donze, Hollie.

In: Infection and Immunity, Vol. 75, No. 4, 01.04.2007, p. 1619-1625.

Research output: Contribution to journalArticle

Fischer, JL, West, J, Agochukwu, N, Suire, C & Hale-Donze, H 2007, 'Induction of host chemotactic response by Encephalitozoon spp.', Infection and Immunity, vol. 75, no. 4, pp. 1619-1625. https://doi.org/10.1128/IAI.01535-06
Fischer, Jeffrey L ; West, Jeffrey ; Agochukwu, Nnenaya ; Suire, Colby ; Hale-Donze, Hollie. / Induction of host chemotactic response by Encephalitozoon spp. In: Infection and Immunity. 2007 ; Vol. 75, No. 4. pp. 1619-1625.
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