Induction of laryngeal cancer cell death by Ent-11-hydroxy-15-oxo-kaur-16- en-19-oic acid

Alexander C. Vlantis, Chun Shan Lo, George G. Chen, Nian Ci Liang, Vivian W.Y. Lui, Kefeng Wu, Yi Feng Deng, Xianling Gong, Yingnian Lu, Michael C.F. Tong, C. Andrew Van Hasselt

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Background. Ent-11-hydroxy-15-oxo-kaur-16-en-19-oic acid (5F) is known to exhibit antitumor activity, but its mechanism is not completely understood. 5F has not been tested in laryngeal cancer. Methods. Two laryngeal cancer cell lines were treated with 5F. Cell death was analyzed by MTT [3-(4,5- dimethylthiozol-2-yl)-2,5-diphenyltetrazolium bromide] and Annexin V assay. Nuclear factor kappa beta (NF-κB)- and apoptosis-related molecules were examined. Results. 5F induced laryngeal cancer cell death in a dose-dependent manner. The Annexin V assay and the measurement of cleavage of procaspase-3 and poly(ADP-ribose) polymerase demonstrated that the 5F-induced cell death was mainly apoptotic. 5F slightly reduced the basal level of NF-κB, but significantly suppressed the inducible NF-κB by reducing its transcriptional activity, protecting its inhibitory subunit IκBα from degradation, and suppressing its level in the nucleus. 5F also inhibited pro-proliferative and anti-apoptotic molecules but promoted pro-apoptotic Bax. Conclusions. 5F induces apoptosis of laryngeal cancer cells by inhibiting NF-κB activation/induction, suppressing pro-proliferative and anti-apoptotic molecules, and promoting pro-apoptotic Bax.

Original languageEnglish (US)
Pages (from-to)1506-1518
Number of pages13
JournalHead and Neck
Volume32
Issue number11
DOIs
StatePublished - Nov 2010
Externally publishedYes

Keywords

  • apoptosis
  • cell death
  • laryngeal cancer
  • nuclear factor kappaB
  • Pteris semipinnata L

ASJC Scopus subject areas

  • Otorhinolaryngology

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