Induction of p57 is required for cell survival when exposed to green tea polyphenols

Stephen Hsu, Fu Shin Yu, Jill Lewis, Baldev Singh, James Borke, Tokio Osaki, Mohammad Athar, George Schuster

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Green tea polyphenols (catechins) are known to induce cell death in many types of tumor cells, but how normal epithelial cells survive in the presence of polyphenols is unknown. We recently reported that green tea polyphenols potently induced a cyclin-dependent kinase inhibitor, p57/(KIP2), only in normal human epithelial cells. In this study, we investigated the correlation between p57 expression and survival/apoptosis by Western blot analysis, caspase 3 assays and morphological analysis. It was demonstrated that, in the cells that lack p57 induction, green tea polyphenols induced Apaf-1 expression along with caspase 3 activation, leading to apoptosis. In contrast, cells with polyphenol-inducible p57 maintained constant levels of Apaf-1 and proliferating cell nuclear antigen (PCNA), with basal caspase 3 activity. Retroviral-transfected, p57-expressing oral carcinoma cells showed significant resistance to green tea polyphenol-induced apoptosis. Our results suggest that p57/KIP2 is a determinant pro-survival factor for cell protection from green tea polyphenol-induced apoptosis.

Original languageEnglish (US)
Pages (from-to)4115-4120
Number of pages6
JournalAnticancer Research
Volume22
Issue number6 C
StatePublished - Nov 1 2002

Keywords

  • Apoptosis
  • Cell cycle
  • EGCG
  • Epithelial cells
  • Oral carcinomas
  • P57
  • Tea

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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