Inflammation-mediated genetic and epigenetic alterations drive cancer development in the neighboring epithelium upon stromal abrogation of TGF-β signaling

B R Achyut, David A Bader, Ana I Robles, Darawalee Wangsa, Curtis C Harris, Thomas Ried, Li Yang

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Deletion of tumor suppressor genes in stromal fibroblasts induces epithelial cancer development, suggesting an important role of stroma in epithelial homoeostasis. However, the underlying mechanisms remain to be elucidated. Here we report that deletion of the gene encoding TGFβ receptor 2 (Tgfbr2) in the stromal fibroblasts (Tgfbr2(fspKO)) induces inflammation and significant DNA damage in the neighboring epithelia of the forestomach. This results in loss or down-regulation of cyclin-dependent kinase inhibitors p15, p16, and p21, which contribute to the development of invasive squamous cell carcinoma (SCC). Anti-inflammation treatment restored p21 expression, delayed tumorigenesis, and increased survival of Tgfbr2(fspKO) mice. Our data demonstrate for the first time that inflammation is a critical player in the epigenetic silencing of p21 in tumor progression. Examination of human esophageal SCC showed a down-regulation of TGFβ receptor 2 (TβRII) in the stromal fibroblasts, as well as increased inflammation, DNA damage, and loss or decreased p15/p16 expression. Our study suggests anti-inflammation may be a new therapeutic option in treating human SCCs with down-regulation of TβRII in the stroma.

Original languageEnglish (US)
Pages (from-to)e1003251
JournalPLoS Genetics
Volume9
Issue number2
DOIs
StatePublished - 2013
Externally publishedYes

Fingerprint

Epigenomics
epigenetics
cancer
epithelium
Epithelium
inflammation
Inflammation
tumor
neoplasms
fibroblasts
Down-Regulation
Fibroblasts
squamous cell carcinoma
DNA
Neoplasms
damage
gene
DNA damage
DNA Damage
homeostasis

Keywords

  • Animals
  • Apoptosis
  • Breast Neoplasms
  • Carcinoma, Squamous Cell
  • Cell Transformation, Neoplastic
  • Cyclin-Dependent Kinase Inhibitor Proteins
  • Down-Regulation
  • Epigenesis, Genetic
  • Epithelial Cells
  • Esophageal Neoplasms
  • Female
  • Fibroblasts
  • Humans
  • Inflammation
  • Mice
  • Protein-Serine-Threonine Kinases
  • Receptors, Transforming Growth Factor beta
  • Signal Transduction
  • Stromal Cells
  • Transforming Growth Factor beta
  • Journal Article
  • Research Support, N.I.H., Intramural

Cite this

Inflammation-mediated genetic and epigenetic alterations drive cancer development in the neighboring epithelium upon stromal abrogation of TGF-β signaling. / Achyut, B R; Bader, David A; Robles, Ana I; Wangsa, Darawalee; Harris, Curtis C; Ried, Thomas; Yang, Li.

In: PLoS Genetics, Vol. 9, No. 2, 2013, p. e1003251.

Research output: Contribution to journalArticle

Achyut, B R ; Bader, David A ; Robles, Ana I ; Wangsa, Darawalee ; Harris, Curtis C ; Ried, Thomas ; Yang, Li. / Inflammation-mediated genetic and epigenetic alterations drive cancer development in the neighboring epithelium upon stromal abrogation of TGF-β signaling. In: PLoS Genetics. 2013 ; Vol. 9, No. 2. pp. e1003251.
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