Inflammatory mediators in fluid extracted from the coronal occlusal dentine of trimmed teeth

Saulo Geraldeli, Yalan Li, Mary M.B. Hogan, Leo S. Tjaderhane, David Henry Pashley, Teresa A. Morgan, M. Bridget Zimmerman, Kim A. Brogden

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Chemokines and cytokines may occur in dentinal fluids in response to local infection and inflammation. To test this hypothesis, we assessed the presence and concentration of inflammatory mediators in fluid extracted from the coronal occlusal dentine of trimmed teeth. Design: Freshly extracted sound, carious, and restored molars were trimmed through the enamel to expose the underlying dentine, etched with 35% phosphoric acid, and rinsed. Fluid was extracted from the coronal occlusal dentine of these trimmed teeth by centrifugation at 2750 × g for 30 min. Results: When assessed by MALDI-TOF, fluid extracted from the coronal occlusal dentine from 16 molars contained at least 117 peaks with different masses suggesting that this fluid was rich with molecules within the appropriate mass range of potential mediators. Indeed, when assessed for chemokines and cytokines, fluid extracted from the coronal occlusal dentine from 25 extracted molars with caries lesions, 10 extracted restored molars with occlusal amalgam, and 77 extracted sound molars contained IL-1β, TNF-α, IL-6, IL-8, IL-12(p70), and IL-10. A significant elevation was found for TNF-α (p = 0.041) in extracted fluid from teeth restored with amalgam fillings. Conclusions: Overall, fluid extracted from the coronal occlusal dentine of trimmed teeth may be useful in identifying proteins and other molecules in dentine and pulpal fluids and determining their role as mediators in the pathogenesis of oral infection and inflammation.

Original languageEnglish (US)
Pages (from-to)264-270
Number of pages7
JournalArchives of Oral Biology
Volume57
Issue number3
DOIs
StatePublished - Mar 1 2012

Fingerprint

Dentin
Tooth
Chemokines
Dentinal Fluid
Cytokines
Inflammation
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Interleukin-12
Dental Enamel
Infection
Interleukin-8
Centrifugation
Interleukin-1
Interleukin-10
Interleukin-6
Proteins

Keywords

  • Chemokines
  • Cytokines
  • Dentinal fluid
  • MALDI-TOF

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Cite this

Geraldeli, S., Li, Y., Hogan, M. M. B., Tjaderhane, L. S., Pashley, D. H., Morgan, T. A., ... Brogden, K. A. (2012). Inflammatory mediators in fluid extracted from the coronal occlusal dentine of trimmed teeth. Archives of Oral Biology, 57(3), 264-270. https://doi.org/10.1016/j.archoralbio.2011.08.012

Inflammatory mediators in fluid extracted from the coronal occlusal dentine of trimmed teeth. / Geraldeli, Saulo; Li, Yalan; Hogan, Mary M.B.; Tjaderhane, Leo S.; Pashley, David Henry; Morgan, Teresa A.; Zimmerman, M. Bridget; Brogden, Kim A.

In: Archives of Oral Biology, Vol. 57, No. 3, 01.03.2012, p. 264-270.

Research output: Contribution to journalArticle

Geraldeli, S, Li, Y, Hogan, MMB, Tjaderhane, LS, Pashley, DH, Morgan, TA, Zimmerman, MB & Brogden, KA 2012, 'Inflammatory mediators in fluid extracted from the coronal occlusal dentine of trimmed teeth', Archives of Oral Biology, vol. 57, no. 3, pp. 264-270. https://doi.org/10.1016/j.archoralbio.2011.08.012
Geraldeli, Saulo ; Li, Yalan ; Hogan, Mary M.B. ; Tjaderhane, Leo S. ; Pashley, David Henry ; Morgan, Teresa A. ; Zimmerman, M. Bridget ; Brogden, Kim A. / Inflammatory mediators in fluid extracted from the coronal occlusal dentine of trimmed teeth. In: Archives of Oral Biology. 2012 ; Vol. 57, No. 3. pp. 264-270.
@article{90e2b7f81b1847ad88ee12fe23d5c8a1,
title = "Inflammatory mediators in fluid extracted from the coronal occlusal dentine of trimmed teeth",
abstract = "Background: Chemokines and cytokines may occur in dentinal fluids in response to local infection and inflammation. To test this hypothesis, we assessed the presence and concentration of inflammatory mediators in fluid extracted from the coronal occlusal dentine of trimmed teeth. Design: Freshly extracted sound, carious, and restored molars were trimmed through the enamel to expose the underlying dentine, etched with 35{\%} phosphoric acid, and rinsed. Fluid was extracted from the coronal occlusal dentine of these trimmed teeth by centrifugation at 2750 × g for 30 min. Results: When assessed by MALDI-TOF, fluid extracted from the coronal occlusal dentine from 16 molars contained at least 117 peaks with different masses suggesting that this fluid was rich with molecules within the appropriate mass range of potential mediators. Indeed, when assessed for chemokines and cytokines, fluid extracted from the coronal occlusal dentine from 25 extracted molars with caries lesions, 10 extracted restored molars with occlusal amalgam, and 77 extracted sound molars contained IL-1β, TNF-α, IL-6, IL-8, IL-12(p70), and IL-10. A significant elevation was found for TNF-α (p = 0.041) in extracted fluid from teeth restored with amalgam fillings. Conclusions: Overall, fluid extracted from the coronal occlusal dentine of trimmed teeth may be useful in identifying proteins and other molecules in dentine and pulpal fluids and determining their role as mediators in the pathogenesis of oral infection and inflammation.",
keywords = "Chemokines, Cytokines, Dentinal fluid, MALDI-TOF",
author = "Saulo Geraldeli and Yalan Li and Hogan, {Mary M.B.} and Tjaderhane, {Leo S.} and Pashley, {David Henry} and Morgan, {Teresa A.} and Zimmerman, {M. Bridget} and Brogden, {Kim A.}",
year = "2012",
month = "3",
day = "1",
doi = "10.1016/j.archoralbio.2011.08.012",
language = "English (US)",
volume = "57",
pages = "264--270",
journal = "Archives of Oral Biology",
issn = "0003-9969",
publisher = "Elsevier Limited",
number = "3",

}

TY - JOUR

T1 - Inflammatory mediators in fluid extracted from the coronal occlusal dentine of trimmed teeth

AU - Geraldeli, Saulo

AU - Li, Yalan

AU - Hogan, Mary M.B.

AU - Tjaderhane, Leo S.

AU - Pashley, David Henry

AU - Morgan, Teresa A.

AU - Zimmerman, M. Bridget

AU - Brogden, Kim A.

PY - 2012/3/1

Y1 - 2012/3/1

N2 - Background: Chemokines and cytokines may occur in dentinal fluids in response to local infection and inflammation. To test this hypothesis, we assessed the presence and concentration of inflammatory mediators in fluid extracted from the coronal occlusal dentine of trimmed teeth. Design: Freshly extracted sound, carious, and restored molars were trimmed through the enamel to expose the underlying dentine, etched with 35% phosphoric acid, and rinsed. Fluid was extracted from the coronal occlusal dentine of these trimmed teeth by centrifugation at 2750 × g for 30 min. Results: When assessed by MALDI-TOF, fluid extracted from the coronal occlusal dentine from 16 molars contained at least 117 peaks with different masses suggesting that this fluid was rich with molecules within the appropriate mass range of potential mediators. Indeed, when assessed for chemokines and cytokines, fluid extracted from the coronal occlusal dentine from 25 extracted molars with caries lesions, 10 extracted restored molars with occlusal amalgam, and 77 extracted sound molars contained IL-1β, TNF-α, IL-6, IL-8, IL-12(p70), and IL-10. A significant elevation was found for TNF-α (p = 0.041) in extracted fluid from teeth restored with amalgam fillings. Conclusions: Overall, fluid extracted from the coronal occlusal dentine of trimmed teeth may be useful in identifying proteins and other molecules in dentine and pulpal fluids and determining their role as mediators in the pathogenesis of oral infection and inflammation.

AB - Background: Chemokines and cytokines may occur in dentinal fluids in response to local infection and inflammation. To test this hypothesis, we assessed the presence and concentration of inflammatory mediators in fluid extracted from the coronal occlusal dentine of trimmed teeth. Design: Freshly extracted sound, carious, and restored molars were trimmed through the enamel to expose the underlying dentine, etched with 35% phosphoric acid, and rinsed. Fluid was extracted from the coronal occlusal dentine of these trimmed teeth by centrifugation at 2750 × g for 30 min. Results: When assessed by MALDI-TOF, fluid extracted from the coronal occlusal dentine from 16 molars contained at least 117 peaks with different masses suggesting that this fluid was rich with molecules within the appropriate mass range of potential mediators. Indeed, when assessed for chemokines and cytokines, fluid extracted from the coronal occlusal dentine from 25 extracted molars with caries lesions, 10 extracted restored molars with occlusal amalgam, and 77 extracted sound molars contained IL-1β, TNF-α, IL-6, IL-8, IL-12(p70), and IL-10. A significant elevation was found for TNF-α (p = 0.041) in extracted fluid from teeth restored with amalgam fillings. Conclusions: Overall, fluid extracted from the coronal occlusal dentine of trimmed teeth may be useful in identifying proteins and other molecules in dentine and pulpal fluids and determining their role as mediators in the pathogenesis of oral infection and inflammation.

KW - Chemokines

KW - Cytokines

KW - Dentinal fluid

KW - MALDI-TOF

UR - http://www.scopus.com/inward/record.url?scp=84857631991&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84857631991&partnerID=8YFLogxK

U2 - 10.1016/j.archoralbio.2011.08.012

DO - 10.1016/j.archoralbio.2011.08.012

M3 - Article

VL - 57

SP - 264

EP - 270

JO - Archives of Oral Biology

JF - Archives of Oral Biology

SN - 0003-9969

IS - 3

ER -