Influence of bilateral cryptorchidism in the mature rat: Alterations in testicular function and serum hormone levels

Brooks Allen Keel, Tom O. Abney

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Mature male Sprague-Dawley rats were divided into two groups. One group was rendered bilaterally cryptorchid and the other served as intact controls. Seven, 14, 21, and 28 days post surgery, five animals from each group were sacrificed, lood samples were collected, and the levels of serum gonadotropins and steroids were subsequently determined by RIA. The testes were removed and used to determine zero time concentrations of testosterone (T), T production in vitro, the zero time concentrations of 17β-estradiol (E2), and the levels of cytoplasmic estrogen receptor (E2R). LH levels in the cryptorchid group increased significantly over the controls at 7 days and remained elevated through 28 days; a similar increase in FSH levels was also detected. Cryptorchidism produced no appreciable effects in the serum steroid levels. No significant difference was detected in the levels of T between the cryptorchid and control groups, and no significant difference in serum E2 levels was detected between the two groups through 21 days; however, a slight but significant decrease in E2 below the control level was observed in the cryptorchid group at 28 days. The concentrations of T in the testis remained relatively constant in the controls, while the levels in the crytorchid animals increased significantly bove controls at 14 days and remained significantly elevated through 28 days. A similar increase above controls in the 3-h Tproduction in vitro was found to be significant at 14 days (P < 0.05) and 28 days (P < 0.01). The levels of testicular E2 remained relatively unchanged up to 28 days, at which time the E2 levels were significantly reduced below control levels. However, when the data were expressed as content per testis, the levels of bothsteroids and T production in the cryptorchid group were found to be reduced compared to control levels. The demonstrationthat bilateral cryptorchidism resulted in a decreased production of testicular steroids suggests damage to or loss of Leydig cells. The cytoplasmic E2R-binding capacity, expressed as femtomolesof E2 per mg cytosol protein, was found to be increased 2- to 4-old over that of the control group; this increase was detectable is early as 7 days post surgery. Thus, concomitant to the reduced steroid production, the E2R-binding capacity, which is localized in the Leydig cell, was dramatically increased. This indicates that Leydig cell responsiveness, as reflected by androgen production, as impaired by cryptorchidism. The adverse effects of cryptorchidism, however, did not extend to all functions of the Leydig cell, since E2R binding was enhanced. The demonstration hat serum gonadotropins increased while serum steroids remained nchanged after cryptorchidism suggests the possibility that testicular factors other than T and E2 might be involved in the feedback control of the hypothalamic-pituitary axis.

Original languageEnglish (US)
Pages (from-to)1226-1233
Number of pages8
JournalEndocrinology
Volume107
Issue number4
DOIs
StatePublished - Jan 1 1980

Fingerprint

Cryptorchidism
Leydig Cells
Steroids
Hormones
Testis
Serum
Gonadotropins
Ambulatory Surgical Procedures
Control Groups
Cytoplasmic and Nuclear Receptors
Estrogen Receptors
Cytosol
Androgens
Sprague Dawley Rats
Testosterone
Estradiol
Proteins

ASJC Scopus subject areas

  • Endocrinology

Cite this

Influence of bilateral cryptorchidism in the mature rat : Alterations in testicular function and serum hormone levels. / Keel, Brooks Allen; Abney, Tom O.

In: Endocrinology, Vol. 107, No. 4, 01.01.1980, p. 1226-1233.

Research output: Contribution to journalArticle

@article{b2a627cb16804c50895b7b3b591377ca,
title = "Influence of bilateral cryptorchidism in the mature rat: Alterations in testicular function and serum hormone levels",
abstract = "Mature male Sprague-Dawley rats were divided into two groups. One group was rendered bilaterally cryptorchid and the other served as intact controls. Seven, 14, 21, and 28 days post surgery, five animals from each group were sacrificed, lood samples were collected, and the levels of serum gonadotropins and steroids were subsequently determined by RIA. The testes were removed and used to determine zero time concentrations of testosterone (T), T production in vitro, the zero time concentrations of 17β-estradiol (E2), and the levels of cytoplasmic estrogen receptor (E2R). LH levels in the cryptorchid group increased significantly over the controls at 7 days and remained elevated through 28 days; a similar increase in FSH levels was also detected. Cryptorchidism produced no appreciable effects in the serum steroid levels. No significant difference was detected in the levels of T between the cryptorchid and control groups, and no significant difference in serum E2 levels was detected between the two groups through 21 days; however, a slight but significant decrease in E2 below the control level was observed in the cryptorchid group at 28 days. The concentrations of T in the testis remained relatively constant in the controls, while the levels in the crytorchid animals increased significantly bove controls at 14 days and remained significantly elevated through 28 days. A similar increase above controls in the 3-h Tproduction in vitro was found to be significant at 14 days (P < 0.05) and 28 days (P < 0.01). The levels of testicular E2 remained relatively unchanged up to 28 days, at which time the E2 levels were significantly reduced below control levels. However, when the data were expressed as content per testis, the levels of bothsteroids and T production in the cryptorchid group were found to be reduced compared to control levels. The demonstrationthat bilateral cryptorchidism resulted in a decreased production of testicular steroids suggests damage to or loss of Leydig cells. The cytoplasmic E2R-binding capacity, expressed as femtomolesof E2 per mg cytosol protein, was found to be increased 2- to 4-old over that of the control group; this increase was detectable is early as 7 days post surgery. Thus, concomitant to the reduced steroid production, the E2R-binding capacity, which is localized in the Leydig cell, was dramatically increased. This indicates that Leydig cell responsiveness, as reflected by androgen production, as impaired by cryptorchidism. The adverse effects of cryptorchidism, however, did not extend to all functions of the Leydig cell, since E2R binding was enhanced. The demonstration hat serum gonadotropins increased while serum steroids remained nchanged after cryptorchidism suggests the possibility that testicular factors other than T and E2 might be involved in the feedback control of the hypothalamic-pituitary axis.",
author = "Keel, {Brooks Allen} and Abney, {Tom O.}",
year = "1980",
month = "1",
day = "1",
doi = "10.1210/endo-107-4-1226",
language = "English (US)",
volume = "107",
pages = "1226--1233",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "4",

}

TY - JOUR

T1 - Influence of bilateral cryptorchidism in the mature rat

T2 - Alterations in testicular function and serum hormone levels

AU - Keel, Brooks Allen

AU - Abney, Tom O.

PY - 1980/1/1

Y1 - 1980/1/1

N2 - Mature male Sprague-Dawley rats were divided into two groups. One group was rendered bilaterally cryptorchid and the other served as intact controls. Seven, 14, 21, and 28 days post surgery, five animals from each group were sacrificed, lood samples were collected, and the levels of serum gonadotropins and steroids were subsequently determined by RIA. The testes were removed and used to determine zero time concentrations of testosterone (T), T production in vitro, the zero time concentrations of 17β-estradiol (E2), and the levels of cytoplasmic estrogen receptor (E2R). LH levels in the cryptorchid group increased significantly over the controls at 7 days and remained elevated through 28 days; a similar increase in FSH levels was also detected. Cryptorchidism produced no appreciable effects in the serum steroid levels. No significant difference was detected in the levels of T between the cryptorchid and control groups, and no significant difference in serum E2 levels was detected between the two groups through 21 days; however, a slight but significant decrease in E2 below the control level was observed in the cryptorchid group at 28 days. The concentrations of T in the testis remained relatively constant in the controls, while the levels in the crytorchid animals increased significantly bove controls at 14 days and remained significantly elevated through 28 days. A similar increase above controls in the 3-h Tproduction in vitro was found to be significant at 14 days (P < 0.05) and 28 days (P < 0.01). The levels of testicular E2 remained relatively unchanged up to 28 days, at which time the E2 levels were significantly reduced below control levels. However, when the data were expressed as content per testis, the levels of bothsteroids and T production in the cryptorchid group were found to be reduced compared to control levels. The demonstrationthat bilateral cryptorchidism resulted in a decreased production of testicular steroids suggests damage to or loss of Leydig cells. The cytoplasmic E2R-binding capacity, expressed as femtomolesof E2 per mg cytosol protein, was found to be increased 2- to 4-old over that of the control group; this increase was detectable is early as 7 days post surgery. Thus, concomitant to the reduced steroid production, the E2R-binding capacity, which is localized in the Leydig cell, was dramatically increased. This indicates that Leydig cell responsiveness, as reflected by androgen production, as impaired by cryptorchidism. The adverse effects of cryptorchidism, however, did not extend to all functions of the Leydig cell, since E2R binding was enhanced. The demonstration hat serum gonadotropins increased while serum steroids remained nchanged after cryptorchidism suggests the possibility that testicular factors other than T and E2 might be involved in the feedback control of the hypothalamic-pituitary axis.

AB - Mature male Sprague-Dawley rats were divided into two groups. One group was rendered bilaterally cryptorchid and the other served as intact controls. Seven, 14, 21, and 28 days post surgery, five animals from each group were sacrificed, lood samples were collected, and the levels of serum gonadotropins and steroids were subsequently determined by RIA. The testes were removed and used to determine zero time concentrations of testosterone (T), T production in vitro, the zero time concentrations of 17β-estradiol (E2), and the levels of cytoplasmic estrogen receptor (E2R). LH levels in the cryptorchid group increased significantly over the controls at 7 days and remained elevated through 28 days; a similar increase in FSH levels was also detected. Cryptorchidism produced no appreciable effects in the serum steroid levels. No significant difference was detected in the levels of T between the cryptorchid and control groups, and no significant difference in serum E2 levels was detected between the two groups through 21 days; however, a slight but significant decrease in E2 below the control level was observed in the cryptorchid group at 28 days. The concentrations of T in the testis remained relatively constant in the controls, while the levels in the crytorchid animals increased significantly bove controls at 14 days and remained significantly elevated through 28 days. A similar increase above controls in the 3-h Tproduction in vitro was found to be significant at 14 days (P < 0.05) and 28 days (P < 0.01). The levels of testicular E2 remained relatively unchanged up to 28 days, at which time the E2 levels were significantly reduced below control levels. However, when the data were expressed as content per testis, the levels of bothsteroids and T production in the cryptorchid group were found to be reduced compared to control levels. The demonstrationthat bilateral cryptorchidism resulted in a decreased production of testicular steroids suggests damage to or loss of Leydig cells. The cytoplasmic E2R-binding capacity, expressed as femtomolesof E2 per mg cytosol protein, was found to be increased 2- to 4-old over that of the control group; this increase was detectable is early as 7 days post surgery. Thus, concomitant to the reduced steroid production, the E2R-binding capacity, which is localized in the Leydig cell, was dramatically increased. This indicates that Leydig cell responsiveness, as reflected by androgen production, as impaired by cryptorchidism. The adverse effects of cryptorchidism, however, did not extend to all functions of the Leydig cell, since E2R binding was enhanced. The demonstration hat serum gonadotropins increased while serum steroids remained nchanged after cryptorchidism suggests the possibility that testicular factors other than T and E2 might be involved in the feedback control of the hypothalamic-pituitary axis.

UR - http://www.scopus.com/inward/record.url?scp=0018970396&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018970396&partnerID=8YFLogxK

U2 - 10.1210/endo-107-4-1226

DO - 10.1210/endo-107-4-1226

M3 - Article

C2 - 6105953

AN - SCOPUS:0018970396

VL - 107

SP - 1226

EP - 1233

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 4

ER -