Influence of chlorhexidine concentration on the durability of etch-and-rinse dentin bonds: A 12-month in vitro study

Lorenzo Breschi, Federica Cammelli, Erika Visintini, Annalisa Mazzoni, Francesca Vita, Marcela Carrilho, Milena Cadenaro, Stephen Foulger, Giovanni Mazzoti, Franklin Chi Meng Tay, Roberto Di Lenarda, David Pashley

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Purpose: To investigate the effect of 0.2% and 2% chlorhexidine (CHX) used as a therapeutic primer on the long-term bond strengths of two etch-and-rinse adhesive systems. Materials and Methods: Adper Scotchbond 1XT (SB1) and XP-Bond (XPB) were evaluated. Etched dentin substrates were assigned to 6 treatment groups: (1) 0.2% CHX + SB1; (2) 2% CHX + SB1; (3) SB1 (control); (4) 0.2% CHX + XPB; (5) 2% CHX + XPB; (6) XPB (control). Composite buildups were made and beams prepared for microtensile bond strength testing. Beams were divided into 3 subgroups and either immediately pulled to failure or stored in artificial saliva for 6 or 12 months prior to testing. Data were evaluated with three-way ANOVA. Additional adhesive interfaces were prepared to investigate nanoleakage expression by TEM. Results: SB1 and XPB showed similar immediate bond strength values with or without CHX pretreatment (p > 0.05). After 12 months, bonds fell from 43.9 ± 9.5 MPa to 20.1 ± 5.4 MPa and from 39.6 ± 9.4 MPa to 14.2 ± 5.0 MPa in control specimens for SB1 and XPB respectively, while bond fell only from 41.9 ± 9.6MPa to 33.2 ± 8.3 MPa and 38.3 ± 8.9 MPa to 26.5 ± 10.9 (for SB1 and XPB, respectively) when 0.2% CHX was previously used. CHX concentration did not affect bond strength values (0.2% vs 2%, p > 0.05). Nanoleakage increased during aging in controls, but reduced silver deposits were found in CHX-treated specimens. Conclusion: Chlorhexidine significantly reduced the loss of bond strength seen in control bonds. Since no bacterial growth was present in the aging conditions, the results of this study suggest that endogenous factors thought to degrade the adhesive interface can be inhibited by CHX. Further in vivo trials should confirm the role of CHX in bond durability.

Original languageEnglish (US)
Pages (from-to)191-198
Number of pages8
JournalJournal of Adhesive Dentistry
Volume11
Issue number3
DOIs
StatePublished - Jan 1 2009

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compound A 12
Chlorhexidine
Dentin
Adhesives
In Vitro Techniques
Artificial Saliva
XP-Bond
Silver
Analysis of Variance
Scotchbond

Keywords

  • Aging
  • Chlorhexidine
  • Dental bonding systems
  • Dentin
  • Hybrid layer

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Breschi, L., Cammelli, F., Visintini, E., Mazzoni, A., Vita, F., Carrilho, M., ... Pashley, D. (2009). Influence of chlorhexidine concentration on the durability of etch-and-rinse dentin bonds: A 12-month in vitro study. Journal of Adhesive Dentistry, 11(3), 191-198. https://doi.org/10.3290/j.jad.a15624

Influence of chlorhexidine concentration on the durability of etch-and-rinse dentin bonds : A 12-month in vitro study. / Breschi, Lorenzo; Cammelli, Federica; Visintini, Erika; Mazzoni, Annalisa; Vita, Francesca; Carrilho, Marcela; Cadenaro, Milena; Foulger, Stephen; Mazzoti, Giovanni; Tay, Franklin Chi Meng; Di Lenarda, Roberto; Pashley, David.

In: Journal of Adhesive Dentistry, Vol. 11, No. 3, 01.01.2009, p. 191-198.

Research output: Contribution to journalArticle

Breschi, L, Cammelli, F, Visintini, E, Mazzoni, A, Vita, F, Carrilho, M, Cadenaro, M, Foulger, S, Mazzoti, G, Tay, FCM, Di Lenarda, R & Pashley, D 2009, 'Influence of chlorhexidine concentration on the durability of etch-and-rinse dentin bonds: A 12-month in vitro study', Journal of Adhesive Dentistry, vol. 11, no. 3, pp. 191-198. https://doi.org/10.3290/j.jad.a15624
Breschi, Lorenzo ; Cammelli, Federica ; Visintini, Erika ; Mazzoni, Annalisa ; Vita, Francesca ; Carrilho, Marcela ; Cadenaro, Milena ; Foulger, Stephen ; Mazzoti, Giovanni ; Tay, Franklin Chi Meng ; Di Lenarda, Roberto ; Pashley, David. / Influence of chlorhexidine concentration on the durability of etch-and-rinse dentin bonds : A 12-month in vitro study. In: Journal of Adhesive Dentistry. 2009 ; Vol. 11, No. 3. pp. 191-198.
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N2 - Purpose: To investigate the effect of 0.2% and 2% chlorhexidine (CHX) used as a therapeutic primer on the long-term bond strengths of two etch-and-rinse adhesive systems. Materials and Methods: Adper Scotchbond 1XT (SB1) and XP-Bond (XPB) were evaluated. Etched dentin substrates were assigned to 6 treatment groups: (1) 0.2% CHX + SB1; (2) 2% CHX + SB1; (3) SB1 (control); (4) 0.2% CHX + XPB; (5) 2% CHX + XPB; (6) XPB (control). Composite buildups were made and beams prepared for microtensile bond strength testing. Beams were divided into 3 subgroups and either immediately pulled to failure or stored in artificial saliva for 6 or 12 months prior to testing. Data were evaluated with three-way ANOVA. Additional adhesive interfaces were prepared to investigate nanoleakage expression by TEM. Results: SB1 and XPB showed similar immediate bond strength values with or without CHX pretreatment (p > 0.05). After 12 months, bonds fell from 43.9 ± 9.5 MPa to 20.1 ± 5.4 MPa and from 39.6 ± 9.4 MPa to 14.2 ± 5.0 MPa in control specimens for SB1 and XPB respectively, while bond fell only from 41.9 ± 9.6MPa to 33.2 ± 8.3 MPa and 38.3 ± 8.9 MPa to 26.5 ± 10.9 (for SB1 and XPB, respectively) when 0.2% CHX was previously used. CHX concentration did not affect bond strength values (0.2% vs 2%, p > 0.05). Nanoleakage increased during aging in controls, but reduced silver deposits were found in CHX-treated specimens. Conclusion: Chlorhexidine significantly reduced the loss of bond strength seen in control bonds. Since no bacterial growth was present in the aging conditions, the results of this study suggest that endogenous factors thought to degrade the adhesive interface can be inhibited by CHX. Further in vivo trials should confirm the role of CHX in bond durability.

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