Influence of endurance exercise training on antioxidant enzymes, tight junction proteins, and inflammatory markers in the rat ileum

Angelia Maleah Holland, Hayden W. Hyatt, Ashley J. Smuder, Kurt J. Sollanek, Aaron B. Morton, Michael D. Roberts, Andreas N. Kavazis

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: This study investigated the effects of endurance exercise training on ileum antioxidant status, as well as tight junction, inflammatory, and nutrient transporter gene expression. Methods: Sprague-Dawley rats (4 month old) were assigned to sedentary (SED) or endurance exercise-training (EXE) groups (n = 8/group). EXE animals were trained on the treadmill for 10 days at a speed of 30 m/min at 0° incline for 60 min/day. SED and EXE animals were sacrificed (24 h after the final training bout) and the ileum was stored for analyses. Results: The ileum of EXE had higher (p < 0.05) antioxidant protein levels of manganese superoxide dismutase and catalase compared to SED with no change (p > 0.05) in the lipid peroxidation biomarker 4-hydroxynonenal. Ileum mRNA expression of the tight junction gene zonulin increased (p < 0.05) and claudin 1 decreased (p < 0.05) in EXE compared to SED, but occludin and zonula occluden 1 were not different (p > 0.05) between SED and EXE. The ileum mRNA expressions of seven nutrient transporters (SLC5A8, SLC7A6, SLC6A19, SLC7A7, SLC27A2, SLC16A10, and SLC15A1) were not different between the two groups (p > 0.05). EXE had lower ileum TNFα mRNA expression (p < 0.05) compared to SED. No changes (p > 0.05) were found in the other inflammatory mRNAs including NFκB, IFNγ, IL6, CCL2, TLR4, and IL10. In addition, no changes in p-p65:p65 were detected. Conclusions: These findings suggest that 10 days of endurance exercise training up-regulates key endogenous antioxidant enzymes, decreases select inflammation markers, and alters select markers of tight junction permeability.

Original languageEnglish (US)
Article number1500
JournalBMC Research Notes
Volume8
Issue number1
DOIs
StatePublished - Sep 30 2015
Externally publishedYes

Fingerprint

Tight Junction Proteins
Ileum
Rats
Durability
Antioxidants
Exercise
Messenger RNA
Enzymes
Nutrients
Tight Junctions
Animals
Exercise equipment
Biomarkers
Gene expression
Interleukin-10
Interleukin-6
Genes
Lipids
Food
Lipid Peroxidation

Keywords

  • Antioxidants
  • Exercise
  • Oxidative stress

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Influence of endurance exercise training on antioxidant enzymes, tight junction proteins, and inflammatory markers in the rat ileum. / Holland, Angelia Maleah; Hyatt, Hayden W.; Smuder, Ashley J.; Sollanek, Kurt J.; Morton, Aaron B.; Roberts, Michael D.; Kavazis, Andreas N.

In: BMC Research Notes, Vol. 8, No. 1, 1500, 30.09.2015.

Research output: Contribution to journalArticle

Holland, Angelia Maleah ; Hyatt, Hayden W. ; Smuder, Ashley J. ; Sollanek, Kurt J. ; Morton, Aaron B. ; Roberts, Michael D. ; Kavazis, Andreas N. / Influence of endurance exercise training on antioxidant enzymes, tight junction proteins, and inflammatory markers in the rat ileum. In: BMC Research Notes. 2015 ; Vol. 8, No. 1.
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AU - Sollanek, Kurt J.

AU - Morton, Aaron B.

AU - Roberts, Michael D.

AU - Kavazis, Andreas N.

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