TY - JOUR
T1 - Influence of growth factors in defined culture medium on in vitro development of mouse embryos
AU - Colver, R. M.
AU - Howe, A. M.
AU - McDonough, P. G.
AU - Boldt, J.
N1 - Funding Information:
Received June 1, 1990; revised and accepted August 14, 1990. * Supported by a Biomedical Research Support grant from the Medical College of Georgia. t Reprint requests: Jeffrey Boldt, Ph.D., Department of Obstetrics and Gynecology, Albany Medical College, 47 New Scotland Avenue, Albany, New York 12208.
PY - 1991
Y1 - 1991
N2 - Two-cell mouse embryos were cultured in Ham's F-10 medium (Gibco, Grand Island, NY) supplemented with various concentrations of transferrin, insulin, epidermal growth factor, platelet-derived growth factor, or fibroblast growth factor. Rates of development and the cell numbers per blastocyst were compared between embryos cultured in Ham's F-10 alone versus growth factor (GF) supplemented media. Rates of development in the presence of GF were inhibited significantly in some cases, and the cell numbers per blastocyst were identical in all groups. When compared with in vivo derived blastocysts, however, all in vitro cultured embryos had significantly less cells per embryo. These results suggest that GF supplementation of media for culture of early preimplantation embryos does not stimulate the rate of embryonic development in vitro.
AB - Two-cell mouse embryos were cultured in Ham's F-10 medium (Gibco, Grand Island, NY) supplemented with various concentrations of transferrin, insulin, epidermal growth factor, platelet-derived growth factor, or fibroblast growth factor. Rates of development and the cell numbers per blastocyst were compared between embryos cultured in Ham's F-10 alone versus growth factor (GF) supplemented media. Rates of development in the presence of GF were inhibited significantly in some cases, and the cell numbers per blastocyst were identical in all groups. When compared with in vivo derived blastocysts, however, all in vitro cultured embryos had significantly less cells per embryo. These results suggest that GF supplementation of media for culture of early preimplantation embryos does not stimulate the rate of embryonic development in vitro.
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U2 - 10.1016/s0015-0282(16)54082-5
DO - 10.1016/s0015-0282(16)54082-5
M3 - Article
C2 - 1986960
AN - SCOPUS:0026078989
SN - 0015-0282
VL - 55
SP - 194
EP - 199
JO - Fertility and sterility
JF - Fertility and sterility
IS - 1
ER -