Influence of hydrostatic pulpal pressure on the microtensile bond strength of all-in-one self-etching adhesives

Keiichi Hosaka, Masatoshi Nakajima, Francesca Monticelli, Marcela Carrilho, Monica Yamauti, Juthatip Aksornmuang, Yoshihiro Nishitani, Franklin Chi Meng Tay, David H. Pashley, Junji Tagami

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25 Scopus citations


Purpose: To evaluate the microtensile bond strength (μTBS) of two all-in-one self-etching adhesive systems and two self-etching adhesives with and without simulated hydrostatic pulpal pressure (PP). Materials and Methods: Flat coronal dentin surfaces of extracted human molars were prepared. Two all-in-one self-etching adhesive systems, One-Up Bond F (OBF; Tokuyama) and Clearfil S3 Bond (Tri-S, Kuraray Medical) and two self-etching primer adhesives, Clearfil Protect Bond (PB; Kuraray) and Clearfil SE Bond (SE; Kuraray) were applied to the dentin surfaces according to manufacturers' instructions under either a pulpal pressure (PP) of zero or 15 cm H 2O. A hybrid resin composite (Clearfil AP-X, Kuraray) was used for the coronal buildup. Specimens bonded under PP were stored in water at 37°C under 15 cm H2O for 24 h. Specimens not bonded under PP were stored under a PP of zero. After storage, the bonded specimens were sectioned into slabs that were trimmed to hourglass-shaped specimens, and were subjected to microtensile bond testing (μTBS). The bond strength data were statistically analyzed using two-way ANOVA and the Holm-Sidak method for multiple comparison tests (α = 0.05). The surface area percentage of different failure modes for each material was also statistically analyzed with three one-way ANOVAs and Tukey's multiple comparison tests. Results: The μTBS of OBF and Tri-S fell significantly under PP. However, in the PB and SE bonded specimens under PP, there were no significant differences compared with the control groups without PP. Conclusions: The μTBS of the two all-in-one adhesive systems decreased when PP was applied. However, the μTBS of both self-etching primer adhesives did not decrease under PP.

Original languageEnglish (US)
Pages (from-to)437-442
Number of pages6
JournalJournal of Adhesive Dentistry
Issue number5
Publication statusPublished - Nov 16 2007



  • All-in-one adhesives
  • Dentin
  • Microtensile bond strength
  • Pulpal pressure

ASJC Scopus subject areas

  • Orthodontics
  • Oral Surgery
  • Periodontics

Cite this

Hosaka, K., Nakajima, M., Monticelli, F., Carrilho, M., Yamauti, M., Aksornmuang, J., ... Tagami, J. (2007). Influence of hydrostatic pulpal pressure on the microtensile bond strength of all-in-one self-etching adhesives. Journal of Adhesive Dentistry, 9(5), 437-442.