Influence of lifestyle choices on risks of CYP1B1 polymorphisms for prostate cancer

Taku Kato, Yutaka Hashimoto, Ryan K. Wong, Yozo Mitsui, Shigekatsu Maekawa, Inik Chang, Varahram Shahryari, Soichiro Yamamura, Shahana Majid, Sharanjot Saini, Z. Laura Tabatabai, Rajvir Dahiya, Takashi Deguchi, Yuichiro Tanaka

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Cytochrome P450 1B1 (CYP1B1) converts xenobiotics to carcinogens and how lifestyle choices may interact with CYP1B1 polymorphisms and affect prostate cancer risk was assessed. Blood genomic DNA from a Caucasian population was analysed at polymorphic sites of the 5′ untranslated region of CYP1B1 using TaqMan geno-typing assays. Overall, drinker status and minor alleles at rs2551188, rs2567206 and rs10175368 were associated with prostate cancer. Linkage was observed between rs2551188, rs2567206, rs2567207 and rs10175368, and the G-C-T-G haplotype (major allele at respective sites) was decreased in cancer. Interestingly when classified by lifestyle factors, no associations of genotypes were found for non-smokers and non-drinkers, whereas on the contrary, minor type at rs2567206 and rs10175368 increased and major G-C-T-G decreased risk for cancer among smokers and drinkers. Interestingly, rs2551188, rs2567206 and rs10175368 minor genotypes correlated with increased tissue CYP1B1 as determined by immunohistochemistry. Further, rs10175368 enhanced luciferase activity and mobility shift show stronger binding of nuclear factor for the minor allele. These results demonstrate smoking and alcohol consumption to modify the risks of CYP1B1 polymorphisms for prostate cancer which may be through rs10175368, and this is of importance in understanding their role in the pathogenesis and as a biomarker for this disease.

Original languageEnglish (US)
Pages (from-to)4676-4687
Number of pages12
JournalJournal of Cellular and Molecular Medicine
Volume22
Issue number10
DOIs
StatePublished - Oct 2018
Externally publishedYes

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Keywords

  • Alcohol consumption
  • Cytochrome P450 1B1
  • Polymorphisms
  • Prostate cancer
  • Smoker

ASJC Scopus subject areas

  • Molecular Medicine
  • Cell Biology

Cite this

Kato, T., Hashimoto, Y., Wong, R. K., Mitsui, Y., Maekawa, S., Chang, I., Shahryari, V., Yamamura, S., Majid, S., Saini, S., Tabatabai, Z. L., Dahiya, R., Deguchi, T., & Tanaka, Y. (2018). Influence of lifestyle choices on risks of CYP1B1 polymorphisms for prostate cancer. Journal of Cellular and Molecular Medicine, 22(10), 4676-4687. https://doi.org/10.1111/jcmm.13696