Influence of matrix-suspended demineralized bone on osseous repair using a critical-sized defect in the rat (Rattus norvegicus) calvarium.

Bryan P. Kalish, George S. Schuster, Mark E. Peacock, Michael F. Cuenin, Gary D. Swiec, Brad J. Potter, Thomas B. Buxton, James C. McPherson

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Demineralized freeze-dried bone (DFDB) in matrix form must be rehydrated with a carrier medium which allows for easy manipulation during periodontal surgery. The purpose of this study was to evaluate how human DFDB suspended in a polyol matrix affects new bone formation in the rat calvarium critical-sized defect (CSD) model. Fifty-five adult male Harlan Sprague-Dawley rats were assigned to 1 of 5 treatment groups: polyol, 100% DFDB, 47% DFDB/polyol, 47% DFDB, or an unfilled control. They were then placed into 8-m calvarial CSDs. The bone donor source company for the DFDB and DFDB/polyol groups was the same. Calvaria were harvested 10 weeks after surgery and evaluated histomorphometrically. The diameter of bone particles from the 3 groups containing DFDB was measured by scanning electron microscopy. There was no statistically significant difference in the percentage of bone fill between any of the groups, although the 100% DFDB group exhibited the most bone fill. The 47% DFDB/polyol and 47% DFDB groups had similar amounts of bone formation. The average size of the demineralized bone particles from the 100% DFDB group was significantly smaller than that of the other 2 groups containing DFDB. Adding a polyol to DFDB produced similar osseous regeneration in the rat calvarium defect model vs DFDB alone. Yet from a clinical standpoint, the polyol enhanced graft handling and stability. Graft particle size may have an effect on bone fill.

Original languageEnglish (US)
Pages (from-to)83-89
Number of pages7
JournalThe Journal of oral implantology
Volume34
Issue number2
DOIs
StatePublished - Jul 2 2008
Externally publishedYes

Fingerprint

Skull
Bone and Bones
Osteogenesis
Transplants
Bone Matrix
polyol
Particle Size

ASJC Scopus subject areas

  • Oral Surgery

Cite this

Influence of matrix-suspended demineralized bone on osseous repair using a critical-sized defect in the rat (Rattus norvegicus) calvarium. / Kalish, Bryan P.; Schuster, George S.; Peacock, Mark E.; Cuenin, Michael F.; Swiec, Gary D.; Potter, Brad J.; Buxton, Thomas B.; McPherson, James C.

In: The Journal of oral implantology, Vol. 34, No. 2, 02.07.2008, p. 83-89.

Research output: Contribution to journalArticle

Kalish, Bryan P. ; Schuster, George S. ; Peacock, Mark E. ; Cuenin, Michael F. ; Swiec, Gary D. ; Potter, Brad J. ; Buxton, Thomas B. ; McPherson, James C. / Influence of matrix-suspended demineralized bone on osseous repair using a critical-sized defect in the rat (Rattus norvegicus) calvarium. In: The Journal of oral implantology. 2008 ; Vol. 34, No. 2. pp. 83-89.
@article{f15bea52510249668ecd90e1fd6b997a,
title = "Influence of matrix-suspended demineralized bone on osseous repair using a critical-sized defect in the rat (Rattus norvegicus) calvarium.",
abstract = "Demineralized freeze-dried bone (DFDB) in matrix form must be rehydrated with a carrier medium which allows for easy manipulation during periodontal surgery. The purpose of this study was to evaluate how human DFDB suspended in a polyol matrix affects new bone formation in the rat calvarium critical-sized defect (CSD) model. Fifty-five adult male Harlan Sprague-Dawley rats were assigned to 1 of 5 treatment groups: polyol, 100{\%} DFDB, 47{\%} DFDB/polyol, 47{\%} DFDB, or an unfilled control. They were then placed into 8-m calvarial CSDs. The bone donor source company for the DFDB and DFDB/polyol groups was the same. Calvaria were harvested 10 weeks after surgery and evaluated histomorphometrically. The diameter of bone particles from the 3 groups containing DFDB was measured by scanning electron microscopy. There was no statistically significant difference in the percentage of bone fill between any of the groups, although the 100{\%} DFDB group exhibited the most bone fill. The 47{\%} DFDB/polyol and 47{\%} DFDB groups had similar amounts of bone formation. The average size of the demineralized bone particles from the 100{\%} DFDB group was significantly smaller than that of the other 2 groups containing DFDB. Adding a polyol to DFDB produced similar osseous regeneration in the rat calvarium defect model vs DFDB alone. Yet from a clinical standpoint, the polyol enhanced graft handling and stability. Graft particle size may have an effect on bone fill.",
author = "Kalish, {Bryan P.} and Schuster, {George S.} and Peacock, {Mark E.} and Cuenin, {Michael F.} and Swiec, {Gary D.} and Potter, {Brad J.} and Buxton, {Thomas B.} and McPherson, {James C.}",
year = "2008",
month = "7",
day = "2",
doi = "10.1563/1548-1336(2008)34[83:IOMDBO]2.0.CO;2",
language = "English (US)",
volume = "34",
pages = "83--89",
journal = "Journal of Oral Implantology",
issn = "0160-6972",
publisher = "Allen Press Inc.",
number = "2",

}

TY - JOUR

T1 - Influence of matrix-suspended demineralized bone on osseous repair using a critical-sized defect in the rat (Rattus norvegicus) calvarium.

AU - Kalish, Bryan P.

AU - Schuster, George S.

AU - Peacock, Mark E.

AU - Cuenin, Michael F.

AU - Swiec, Gary D.

AU - Potter, Brad J.

AU - Buxton, Thomas B.

AU - McPherson, James C.

PY - 2008/7/2

Y1 - 2008/7/2

N2 - Demineralized freeze-dried bone (DFDB) in matrix form must be rehydrated with a carrier medium which allows for easy manipulation during periodontal surgery. The purpose of this study was to evaluate how human DFDB suspended in a polyol matrix affects new bone formation in the rat calvarium critical-sized defect (CSD) model. Fifty-five adult male Harlan Sprague-Dawley rats were assigned to 1 of 5 treatment groups: polyol, 100% DFDB, 47% DFDB/polyol, 47% DFDB, or an unfilled control. They were then placed into 8-m calvarial CSDs. The bone donor source company for the DFDB and DFDB/polyol groups was the same. Calvaria were harvested 10 weeks after surgery and evaluated histomorphometrically. The diameter of bone particles from the 3 groups containing DFDB was measured by scanning electron microscopy. There was no statistically significant difference in the percentage of bone fill between any of the groups, although the 100% DFDB group exhibited the most bone fill. The 47% DFDB/polyol and 47% DFDB groups had similar amounts of bone formation. The average size of the demineralized bone particles from the 100% DFDB group was significantly smaller than that of the other 2 groups containing DFDB. Adding a polyol to DFDB produced similar osseous regeneration in the rat calvarium defect model vs DFDB alone. Yet from a clinical standpoint, the polyol enhanced graft handling and stability. Graft particle size may have an effect on bone fill.

AB - Demineralized freeze-dried bone (DFDB) in matrix form must be rehydrated with a carrier medium which allows for easy manipulation during periodontal surgery. The purpose of this study was to evaluate how human DFDB suspended in a polyol matrix affects new bone formation in the rat calvarium critical-sized defect (CSD) model. Fifty-five adult male Harlan Sprague-Dawley rats were assigned to 1 of 5 treatment groups: polyol, 100% DFDB, 47% DFDB/polyol, 47% DFDB, or an unfilled control. They were then placed into 8-m calvarial CSDs. The bone donor source company for the DFDB and DFDB/polyol groups was the same. Calvaria were harvested 10 weeks after surgery and evaluated histomorphometrically. The diameter of bone particles from the 3 groups containing DFDB was measured by scanning electron microscopy. There was no statistically significant difference in the percentage of bone fill between any of the groups, although the 100% DFDB group exhibited the most bone fill. The 47% DFDB/polyol and 47% DFDB groups had similar amounts of bone formation. The average size of the demineralized bone particles from the 100% DFDB group was significantly smaller than that of the other 2 groups containing DFDB. Adding a polyol to DFDB produced similar osseous regeneration in the rat calvarium defect model vs DFDB alone. Yet from a clinical standpoint, the polyol enhanced graft handling and stability. Graft particle size may have an effect on bone fill.

UR - http://www.scopus.com/inward/record.url?scp=45849112865&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=45849112865&partnerID=8YFLogxK

U2 - 10.1563/1548-1336(2008)34[83:IOMDBO]2.0.CO;2

DO - 10.1563/1548-1336(2008)34[83:IOMDBO]2.0.CO;2

M3 - Article

C2 - 18478903

AN - SCOPUS:45849112865

VL - 34

SP - 83

EP - 89

JO - Journal of Oral Implantology

JF - Journal of Oral Implantology

SN - 0160-6972

IS - 2

ER -