Influence of protein size on surface-enhanced Raman scattering (SERS) spectra in binary protein mixtures

Ertug Avci, Mustafa Culha

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The size-dependent interactions of eight blood proteins with silver nanoparticles (AgNPs) in their binary mixtures were investigated using surface-enhanced Raman scattering (SERS). Principal component analysis (PCA) was performed on the SERS spectra of each binary mixture, and the differentiation ability of the mixtures was tested. It was found that the effect of relative concentration change on the SERS spectra of the binary mixtures of small proteins could be detected using PCA. However, this change was not observed with the binary mixtures of large proteins. This study demonstrated that the relative interactions of the smaller proteins with an average size of 50 nm AgNPs smaller than the large proteins could be monitored, and this information can be used for the detection of proteins in protein mixtures.

Original languageEnglish (US)
Pages (from-to)890-899
Number of pages10
JournalApplied Spectroscopy
Volume68
Issue number8
DOIs
StatePublished - 2014
Externally publishedYes

ASJC Scopus subject areas

  • Instrumentation
  • Spectroscopy

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